Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in Bacillus subtilis Article Swipe
Marco Harms
,
Chelsea Kaden
,
Larissa M. Busch
,
Vishnu M. Dhople
,
Ulf Gerth
,
Manuela Gesell Salazar
,
Stephan Michalik
,
Zhanetta Zhatarova
,
Uwe Völker
,
Alexander Reder
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.09.04.674265
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.09.04.674265
A sudden increase in temperature triggers Bacillus subtilis to activate expression of stress-specific heat shock proteins of the CtsR ( c lass t hree s tress gene r epressor) regulon to withstand the adverse conditions. Key members of this regulon, such as ATPases, proteolytic subunits and their adaptors, which can assemble to the functional Clp protease system, perform crucial roles in maintaining cellular proteostasis, while their transcription is repressed by CtsR during vegetative growth. Upon heat shock, a conformational change in a thermosensing glycine-rich loop causes CtsR to detach from its DNA operators, enabling the transcriptional activation of the regulon. Novel data from a clpX -deficient strain demonstrated that in addition, the presence of the ATPase ClpX is essential for the CtsR dissociation from its DNA binding site. To further elucidate this role of ClpX, we constructed a conditional clpX strain, in which clpX induction is decoupled from its native transcriptional control. This conditional expression system mimicked a clpX- deficient phenotype under non-inducing conditions and restored the wild-type phenotype upon induction. Our results indicate that the full induction of the CtsR regulon, particularly clpE , requires both heat and the presence of ClpX, thereby extending the current model for the transcriptional activation of genes repressed by CtsR.
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- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2025.09.04.674265
- https://www.biorxiv.org/content/biorxiv/early/2025/09/04/2025.09.04.674265.full.pdf
- OA Status
- green
- References
- 49
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4414028589
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- OpenAlex ID
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https://openalex.org/W4414028589Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2025.09.04.674265Digital Object Identifier
- Title
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Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress in Bacillus subtilisWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-09-04Full publication date if available
- Authors
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Marco Harms, Chelsea Kaden, Larissa M. Busch, Vishnu M. Dhople, Ulf Gerth, Manuela Gesell Salazar, Stephan Michalik, Zhanetta Zhatarova, Uwe Völker, Alexander RederList of authors in order
- Landing page
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https://doi.org/10.1101/2025.09.04.674265Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2025/09/04/2025.09.04.674265.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2025/09/04/2025.09.04.674265.full.pdfDirect OA link when available
- Concepts
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Bacillus subtilis, Heat stress, Dissociation (chemistry), Operator (biology), Stress (linguistics), Chemistry, Biological system, Biology, Biochemistry, Physics, Atmospheric sciences, Genetics, Physical chemistry, Transcription factor, Bacteria, Gene, Repressor, Philosophy, LinguisticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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49Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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