bioRxiv (Cold Spring Harbor Laboratory)
Resident and recruited macrophages differentially contribute to cardiac healing after myocardial ischemia
May 2023 • Tobias Weinberger, Denise Messerer, Markus Joppich, Maximilian Fischer, Clarisabel Garcia, Konda Kumaraswami, Vanessa Wimmler, Sonja Ablinger, Saskia…
ABSTRACT Cardiac macrophages are heterogenous in phenotype and functions, which has been associated with differences in their ontogeny. Despite extensive research, our understanding of the precise role of different subsets of macrophages in ischemia/reperfusion injury remains incomplete. We here investigated macrophage lineages and ablated tissue macrophages in homeostasis and after I/R injury in a CSF1R-dependent manner. Genomic deletion of a fms-intronic regulatory element (FIRE) in the Csf1r locus resulted in s…