Resource-efficient pooled sequencing expands translational impact in solid tumors Article Swipe
Renzo G. DiNatale
,
Roy Mano
,
Vladimir Makarov
,
Nicole Rusk
,
Esther Drill
,
Andrew Winer
,
Alexander Sankin
,
Angela Yoo
,
Benjamin Freeman
,
James J. Hsieh
,
Ying‐Bei Chen
,
Jonathan Coleman
,
Michael Berger
,
Irina Ostrovnaya
,
Timothy A. Chan
,
Paul Russo
,
Ed Reznik
,
A. Ari Hakimi
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.06.06.447265
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.06.06.447265
Intratumoral genetic heterogeneity (ITH) poses a significant challenge to utilizing sequencing for decision making in the management of cancer. Although sequencing of multiple tumor regions can address the pitfalls of ITH, it does so at a significant increase in cost and resource utilization. We propose a pooled multiregional sequencing strategy, whereby DNA aliquots from multiple tumor regions are mixed prior to sequencing, as a cost-effective strategy to boost translational value by addressing ITH while preserving valuable residual tissue for secondary analysis. Focusing on kidney cancer, we demonstrate that DNA pooling from as few as two regions significantly increases mutation detection while reducing clonality misattribution. This leads to an increased fraction of patients identified with therapeutically actionable mutations, improved patient risk stratification, and improved inference of evolutionary trajectories with an accuracy comparable to bona fide multiregional sequencing. The same approach applied to non-small-cell lung cancer data substantially improves tumor mutational burden (TMB) detection. Our findings demonstrate that pooled DNA sequencing strategies are a cost-effective alternative to address intrinsic genetic heterogeneity in clinical settings.
Related Topics
Concepts
DNA sequencing
Pooling
Computational biology
Biology
Exome sequencing
Deep sequencing
Mutation
Computer science
Genetics
Genome
DNA
Gene
Artificial intelligence
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2021.06.06.447265
- https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdf
- OA Status
- green
- References
- 33
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3167032502
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3167032502Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2021.06.06.447265Digital Object Identifier
- Title
-
Resource-efficient pooled sequencing expands translational impact in solid tumorsWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-06-07Full publication date if available
- Authors
-
Renzo G. DiNatale, Roy Mano, Vladimir Makarov, Nicole Rusk, Esther Drill, Andrew Winer, Alexander Sankin, Angela Yoo, Benjamin Freeman, James J. Hsieh, Ying‐Bei Chen, Jonathan Coleman, Michael Berger, Irina Ostrovnaya, Timothy A. Chan, Paul Russo, Ed Reznik, A. Ari HakimiList of authors in order
- Landing page
-
https://doi.org/10.1101/2021.06.06.447265Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdfDirect OA link when available
- Concepts
-
DNA sequencing, Pooling, Computational biology, Biology, Exome sequencing, Deep sequencing, Mutation, Computer science, Genetics, Genome, DNA, Gene, Artificial intelligenceTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- References (count)
-
33Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3167032502 |
|---|---|
| doi | https://doi.org/10.1101/2021.06.06.447265 |
| ids.doi | https://doi.org/10.1101/2021.06.06.447265 |
| ids.mag | 3167032502 |
| ids.openalex | https://openalex.org/W3167032502 |
| fwci | 0.0 |
| type | preprint |
| title | Resource-efficient pooled sequencing expands translational impact in solid tumors |
| biblio.issue | |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T11287 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 1.0 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1306 |
| topics[0].subfield.display_name | Cancer Research |
| topics[0].display_name | Cancer Genomics and Diagnostics |
| topics[1].id | https://openalex.org/T11369 |
| topics[1].field.id | https://openalex.org/fields/27 |
| topics[1].field.display_name | Medicine |
| topics[1].score | 0.9934999942779541 |
| topics[1].domain.id | https://openalex.org/domains/4 |
| topics[1].domain.display_name | Health Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2734 |
| topics[1].subfield.display_name | Pathology and Forensic Medicine |
| topics[1].display_name | Genetic factors in colorectal cancer |
| topics[2].id | https://openalex.org/T11764 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9922999739646912 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1311 |
| topics[2].subfield.display_name | Genetics |
| topics[2].display_name | Evolution and Genetic Dynamics |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C51679486 |
| concepts[0].level | 3 |
| concepts[0].score | 0.6339681148529053 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q380546 |
| concepts[0].display_name | DNA sequencing |
| concepts[1].id | https://openalex.org/C70437156 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6247189044952393 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q7228652 |
| concepts[1].display_name | Pooling |
| concepts[2].id | https://openalex.org/C70721500 |
| concepts[2].level | 1 |
| concepts[2].score | 0.5341253280639648 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[2].display_name | Computational biology |
| concepts[3].id | https://openalex.org/C86803240 |
| concepts[3].level | 0 |
| concepts[3].score | 0.5325528383255005 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[3].display_name | Biology |
| concepts[4].id | https://openalex.org/C16671776 |
| concepts[4].level | 4 |
| concepts[4].score | 0.46923571825027466 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q5420592 |
| concepts[4].display_name | Exome sequencing |
| concepts[5].id | https://openalex.org/C132917006 |
| concepts[5].level | 4 |
| concepts[5].score | 0.4557698369026184 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q5250436 |
| concepts[5].display_name | Deep sequencing |
| concepts[6].id | https://openalex.org/C501734568 |
| concepts[6].level | 3 |
| concepts[6].score | 0.32283738255500793 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q42918 |
| concepts[6].display_name | Mutation |
| concepts[7].id | https://openalex.org/C41008148 |
| concepts[7].level | 0 |
| concepts[7].score | 0.2955179214477539 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q21198 |
| concepts[7].display_name | Computer science |
| concepts[8].id | https://openalex.org/C54355233 |
| concepts[8].level | 1 |
| concepts[8].score | 0.2481592893600464 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[8].display_name | Genetics |
| concepts[9].id | https://openalex.org/C141231307 |
| concepts[9].level | 3 |
| concepts[9].score | 0.20493358373641968 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q7020 |
| concepts[9].display_name | Genome |
| concepts[10].id | https://openalex.org/C552990157 |
| concepts[10].level | 2 |
| concepts[10].score | 0.18709754943847656 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7430 |
| concepts[10].display_name | DNA |
| concepts[11].id | https://openalex.org/C104317684 |
| concepts[11].level | 2 |
| concepts[11].score | 0.18466034531593323 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[11].display_name | Gene |
| concepts[12].id | https://openalex.org/C154945302 |
| concepts[12].level | 1 |
| concepts[12].score | 0.08066380023956299 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q11660 |
| concepts[12].display_name | Artificial intelligence |
| keywords[0].id | https://openalex.org/keywords/dna-sequencing |
| keywords[0].score | 0.6339681148529053 |
| keywords[0].display_name | DNA sequencing |
| keywords[1].id | https://openalex.org/keywords/pooling |
| keywords[1].score | 0.6247189044952393 |
| keywords[1].display_name | Pooling |
| keywords[2].id | https://openalex.org/keywords/computational-biology |
| keywords[2].score | 0.5341253280639648 |
| keywords[2].display_name | Computational biology |
| keywords[3].id | https://openalex.org/keywords/biology |
| keywords[3].score | 0.5325528383255005 |
| keywords[3].display_name | Biology |
| keywords[4].id | https://openalex.org/keywords/exome-sequencing |
| keywords[4].score | 0.46923571825027466 |
| keywords[4].display_name | Exome sequencing |
| keywords[5].id | https://openalex.org/keywords/deep-sequencing |
| keywords[5].score | 0.4557698369026184 |
| keywords[5].display_name | Deep sequencing |
| keywords[6].id | https://openalex.org/keywords/mutation |
| keywords[6].score | 0.32283738255500793 |
| keywords[6].display_name | Mutation |
| keywords[7].id | https://openalex.org/keywords/computer-science |
| keywords[7].score | 0.2955179214477539 |
| keywords[7].display_name | Computer science |
| keywords[8].id | https://openalex.org/keywords/genetics |
| keywords[8].score | 0.2481592893600464 |
| keywords[8].display_name | Genetics |
| keywords[9].id | https://openalex.org/keywords/genome |
| keywords[9].score | 0.20493358373641968 |
| keywords[9].display_name | Genome |
| keywords[10].id | https://openalex.org/keywords/dna |
| keywords[10].score | 0.18709754943847656 |
| keywords[10].display_name | DNA |
| keywords[11].id | https://openalex.org/keywords/gene |
| keywords[11].score | 0.18466034531593323 |
| keywords[11].display_name | Gene |
| keywords[12].id | https://openalex.org/keywords/artificial-intelligence |
| keywords[12].score | 0.08066380023956299 |
| keywords[12].display_name | Artificial intelligence |
| language | en |
| locations[0].id | doi:10.1101/2021.06.06.447265 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4306402567 |
| locations[0].source.issn | |
| locations[0].source.type | repository |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| locations[0].source.host_organization | https://openalex.org/I2750212522 |
| locations[0].source.host_organization_name | Cold Spring Harbor Laboratory |
| locations[0].source.host_organization_lineage | https://openalex.org/I2750212522 |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdf |
| locations[0].version | acceptedVersion |
| locations[0].raw_type | posted-content |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | False |
| locations[0].raw_source_name | |
| locations[0].landing_page_url | https://doi.org/10.1101/2021.06.06.447265 |
| indexed_in | crossref |
| authorships[0].author.id | https://openalex.org/A5036125588 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-7867-2881 |
| authorships[0].author.display_name | Renzo G. DiNatale |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[0].affiliations[0].raw_affiliation_string | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[0].affiliations[1].institution_ids | https://openalex.org/I1334819555 |
| authorships[0].affiliations[1].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[0].affiliations[2].institution_ids | https://openalex.org/I1334819555 |
| authorships[0].affiliations[2].raw_affiliation_string | Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA |
| authorships[0].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[0].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[0].institutions[0].type | healthcare |
| authorships[0].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Renzo G. DiNatale |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA, Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA, Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[1].author.id | https://openalex.org/A5069533367 |
| authorships[1].author.orcid | https://orcid.org/0000-0003-3855-0211 |
| authorships[1].author.display_name | Roy Mano |
| authorships[1].countries | US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[1].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[1].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[1].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[1].institutions[0].type | healthcare |
| authorships[1].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[1].institutions[0].country_code | US |
| authorships[1].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Roy Mano |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[2].author.id | https://openalex.org/A5103230255 |
| authorships[2].author.orcid | https://orcid.org/0000-0001-8026-824X |
| authorships[2].author.display_name | Vladimir Makarov |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[2].affiliations[0].raw_affiliation_string | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[2].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[2].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[2].institutions[0].type | healthcare |
| authorships[2].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Vladimir Makarov |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[3].author.id | https://openalex.org/A5047614639 |
| authorships[3].author.orcid | https://orcid.org/0000-0003-2663-6288 |
| authorships[3].author.display_name | Nicole Rusk |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[3].affiliations[0].raw_affiliation_string | Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA |
| authorships[3].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[3].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[3].institutions[0].type | healthcare |
| authorships[3].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Nicole Rusk |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA |
| authorships[4].author.id | https://openalex.org/A5048118946 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-3315-4538 |
| authorships[4].author.display_name | Esther Drill |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[4].affiliations[0].raw_affiliation_string | Biostatistics, Epiemiology & Biostatistics Department, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[4].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[4].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[4].institutions[0].type | healthcare |
| authorships[4].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Esther Drill |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Biostatistics, Epiemiology & Biostatistics Department, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[5].author.id | https://openalex.org/A5103229424 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-1378-6845 |
| authorships[5].author.display_name | Andrew Winer |
| authorships[5].affiliations[0].raw_affiliation_string | Dpartmnt of Urology, SUNY Downstat Halth Scincs Univrsity, Brooklyn, NY |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Andrew Winer |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Dpartmnt of Urology, SUNY Downstat Halth Scincs Univrsity, Brooklyn, NY |
| authorships[6].author.id | https://openalex.org/A5002819180 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-8296-4115 |
| authorships[6].author.display_name | Alexander Sankin |
| authorships[6].countries | US |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I129975664 |
| authorships[6].affiliations[0].raw_affiliation_string | Department o Urology, Monteiore Medical Center and Albert Einstein College o Medicine, Bronx, NY |
| authorships[6].institutions[0].id | https://openalex.org/I129975664 |
| authorships[6].institutions[0].ror | https://ror.org/05cf8a891 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I129975664, https://openalex.org/I4210112371 |
| authorships[6].institutions[0].country_code | US |
| authorships[6].institutions[0].display_name | Albert Einstein College of Medicine |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Alexander Sankin |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Department o Urology, Monteiore Medical Center and Albert Einstein College o Medicine, Bronx, NY |
| authorships[7].author.id | https://openalex.org/A5065689083 |
| authorships[7].author.orcid | |
| authorships[7].author.display_name | Angela Yoo |
| authorships[7].countries | US |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[7].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[7].affiliations[1].raw_affiliation_string | Dpartmnt of Urology, SUNY Downstat Halth Scincs Univrsity, Brooklyn, NY |
| authorships[7].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[7].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[7].institutions[0].type | healthcare |
| authorships[7].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[7].institutions[0].country_code | US |
| authorships[7].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Angela Yoo |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | Dpartmnt of Urology, SUNY Downstat Halth Scincs Univrsity, Brooklyn, NY, Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[8].author.id | https://openalex.org/A5001505851 |
| authorships[8].author.orcid | https://orcid.org/0000-0001-6138-9169 |
| authorships[8].author.display_name | Benjamin Freeman |
| authorships[8].countries | US |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[8].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[8].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[8].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[8].institutions[0].type | healthcare |
| authorships[8].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[8].institutions[0].country_code | US |
| authorships[8].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | Benjamin A. Freeman |
| authorships[8].is_corresponding | False |
| authorships[8].raw_affiliation_strings | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[9].author.id | https://openalex.org/A5021734597 |
| authorships[9].author.orcid | https://orcid.org/0000-0003-3595-0551 |
| authorships[9].author.display_name | James J. Hsieh |
| authorships[9].affiliations[0].raw_affiliation_string | Department of Medicine, University of Washinton, Washinton D.C., USA |
| authorships[9].author_position | middle |
| authorships[9].raw_author_name | James J. Hsieh |
| authorships[9].is_corresponding | False |
| authorships[9].raw_affiliation_strings | Department of Medicine, University of Washinton, Washinton D.C., USA |
| authorships[10].author.id | https://openalex.org/A5003119579 |
| authorships[10].author.orcid | https://orcid.org/0000-0001-5207-3648 |
| authorships[10].author.display_name | Ying‐Bei Chen |
| authorships[10].countries | US |
| authorships[10].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[10].affiliations[0].raw_affiliation_string | Department of Patology, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[10].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[10].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[10].institutions[0].type | healthcare |
| authorships[10].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[10].institutions[0].country_code | US |
| authorships[10].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[10].author_position | middle |
| authorships[10].raw_author_name | Ying-Bei Chen |
| authorships[10].is_corresponding | False |
| authorships[10].raw_affiliation_strings | Department of Patology, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[11].author.id | https://openalex.org/A5081581200 |
| authorships[11].author.orcid | https://orcid.org/0000-0002-6428-7835 |
| authorships[11].author.display_name | Jonathan Coleman |
| authorships[11].countries | US |
| authorships[11].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[11].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[11].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[11].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[11].institutions[0].type | healthcare |
| authorships[11].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[11].institutions[0].country_code | US |
| authorships[11].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[11].author_position | middle |
| authorships[11].raw_author_name | Jonathan A. Coleman |
| authorships[11].is_corresponding | False |
| authorships[11].raw_affiliation_strings | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[12].author.id | https://openalex.org/A5073462462 |
| authorships[12].author.orcid | https://orcid.org/0000-0002-6480-1494 |
| authorships[12].author.display_name | Michael Berger |
| authorships[12].countries | US |
| authorships[12].affiliations[0].institution_ids | https://openalex.org/I1334819555, https://openalex.org/I4210150208 |
| authorships[12].affiliations[0].raw_affiliation_string | Center for Molecular Oncology, Memoral Sloan Ketterng Cancer Center, New York, USA |
| authorships[12].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[12].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[12].institutions[0].type | healthcare |
| authorships[12].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[12].institutions[0].country_code | US |
| authorships[12].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[12].institutions[1].id | https://openalex.org/I4210150208 |
| authorships[12].institutions[1].ror | https://ror.org/04rtkc841 |
| authorships[12].institutions[1].type | company |
| authorships[12].institutions[1].lineage | https://openalex.org/I4210150208 |
| authorships[12].institutions[1].country_code | US |
| authorships[12].institutions[1].display_name | Molecular Oncology (United States) |
| authorships[12].author_position | middle |
| authorships[12].raw_author_name | Michael Berger |
| authorships[12].is_corresponding | False |
| authorships[12].raw_affiliation_strings | Center for Molecular Oncology, Memoral Sloan Ketterng Cancer Center, New York, USA |
| authorships[13].author.id | https://openalex.org/A5053307625 |
| authorships[13].author.orcid | https://orcid.org/0000-0003-1783-0070 |
| authorships[13].author.display_name | Irina Ostrovnaya |
| authorships[13].countries | US |
| authorships[13].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[13].affiliations[0].raw_affiliation_string | Biostatistics, Epiemiology & Biostatistics Department, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[13].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[13].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[13].institutions[0].type | healthcare |
| authorships[13].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[13].institutions[0].country_code | US |
| authorships[13].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[13].author_position | middle |
| authorships[13].raw_author_name | Irina Ostrovnaya |
| authorships[13].is_corresponding | False |
| authorships[13].raw_affiliation_strings | Biostatistics, Epiemiology & Biostatistics Department, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[14].author.id | https://openalex.org/A5080223712 |
| authorships[14].author.orcid | https://orcid.org/0000-0002-9265-0283 |
| authorships[14].author.display_name | Timothy A. Chan |
| authorships[14].countries | US |
| authorships[14].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[14].affiliations[0].raw_affiliation_string | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[14].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[14].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[14].institutions[0].type | healthcare |
| authorships[14].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[14].institutions[0].country_code | US |
| authorships[14].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[14].author_position | middle |
| authorships[14].raw_author_name | Timothy A. Chan |
| authorships[14].is_corresponding | False |
| authorships[14].raw_affiliation_strings | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[15].author.id | https://openalex.org/A5030709443 |
| authorships[15].author.orcid | https://orcid.org/0000-0001-7487-6783 |
| authorships[15].author.display_name | Paul Russo |
| authorships[15].countries | US |
| authorships[15].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[15].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[15].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[15].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[15].institutions[0].type | healthcare |
| authorships[15].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[15].institutions[0].country_code | US |
| authorships[15].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[15].author_position | middle |
| authorships[15].raw_author_name | Paul Russo |
| authorships[15].is_corresponding | False |
| authorships[15].raw_affiliation_strings | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[16].author.id | https://openalex.org/A5081768872 |
| authorships[16].author.orcid | https://orcid.org/0000-0002-6511-5947 |
| authorships[16].author.display_name | Ed Reznik |
| authorships[16].countries | US |
| authorships[16].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[16].affiliations[0].raw_affiliation_string | Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA |
| authorships[16].affiliations[1].institution_ids | https://openalex.org/I1334819555, https://openalex.org/I4210150208 |
| authorships[16].affiliations[1].raw_affiliation_string | Center for Molecular Oncology, Memoral Sloan Ketterng Cancer Center, New York, USA |
| authorships[16].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[16].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[16].institutions[0].type | healthcare |
| authorships[16].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[16].institutions[0].country_code | US |
| authorships[16].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[16].institutions[1].id | https://openalex.org/I4210150208 |
| authorships[16].institutions[1].ror | https://ror.org/04rtkc841 |
| authorships[16].institutions[1].type | company |
| authorships[16].institutions[1].lineage | https://openalex.org/I4210150208 |
| authorships[16].institutions[1].country_code | US |
| authorships[16].institutions[1].display_name | Molecular Oncology (United States) |
| authorships[16].author_position | middle |
| authorships[16].raw_author_name | Ed Reznik |
| authorships[16].is_corresponding | True |
| authorships[16].raw_affiliation_strings | Center for Molecular Oncology, Memoral Sloan Ketterng Cancer Center, New York, USA, Computtionl Oncology Service, Epidemiology & Biosttistics Deprtment, Memoril Slon Kettering Cncer Center, New York, USA |
| authorships[17].author.id | https://openalex.org/A5089094298 |
| authorships[17].author.orcid | https://orcid.org/0000-0002-0930-8824 |
| authorships[17].author.display_name | A. Ari Hakimi |
| authorships[17].countries | US |
| authorships[17].affiliations[0].institution_ids | https://openalex.org/I1334819555 |
| authorships[17].affiliations[0].raw_affiliation_string | Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| authorships[17].affiliations[1].institution_ids | https://openalex.org/I1334819555 |
| authorships[17].affiliations[1].raw_affiliation_string | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA |
| authorships[17].institutions[0].id | https://openalex.org/I1334819555 |
| authorships[17].institutions[0].ror | https://ror.org/02yrq0923 |
| authorships[17].institutions[0].type | healthcare |
| authorships[17].institutions[0].lineage | https://openalex.org/I1334819555 |
| authorships[17].institutions[0].country_code | US |
| authorships[17].institutions[0].display_name | Memorial Sloan Kettering Cancer Center |
| authorships[17].author_position | last |
| authorships[17].raw_author_name | A. Ari Hakimi |
| authorships[17].is_corresponding | True |
| authorships[17].raw_affiliation_strings | Immunogenomics and Precision Oncology Platform, Memorial Sloan Kettering Cancer Center, New York, USA, Urology Servie, Department of Surgery, Memorial Sloan Kettering Caner Center, New York, USA |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdf |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Resource-efficient pooled sequencing expands translational impact in solid tumors |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11287 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 1.0 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1306 |
| primary_topic.subfield.display_name | Cancer Research |
| primary_topic.display_name | Cancer Genomics and Diagnostics |
| related_works | https://openalex.org/W2760231366, https://openalex.org/W3029015202, https://openalex.org/W3031411603, https://openalex.org/W2104595023, https://openalex.org/W1539933040, https://openalex.org/W3006152679, https://openalex.org/W1791825763, https://openalex.org/W2003746353, https://openalex.org/W2054261324, https://openalex.org/W2413391814 |
| cited_by_count | 0 |
| locations_count | 1 |
| best_oa_location.id | doi:10.1101/2021.06.06.447265 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4306402567 |
| best_oa_location.source.issn | |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| best_oa_location.source.host_organization | https://openalex.org/I2750212522 |
| best_oa_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| best_oa_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdf |
| best_oa_location.version | acceptedVersion |
| best_oa_location.raw_type | posted-content |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | |
| best_oa_location.landing_page_url | https://doi.org/10.1101/2021.06.06.447265 |
| primary_location.id | doi:10.1101/2021.06.06.447265 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4306402567 |
| primary_location.source.issn | |
| primary_location.source.type | repository |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| primary_location.source.host_organization | https://openalex.org/I2750212522 |
| primary_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| primary_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2021/06/07/2021.06.06.447265.full.pdf |
| primary_location.version | acceptedVersion |
| primary_location.raw_type | posted-content |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | False |
| primary_location.raw_source_name | |
| primary_location.landing_page_url | https://doi.org/10.1101/2021.06.06.447265 |
| publication_date | 2021-06-07 |
| publication_year | 2021 |
| referenced_works | https://openalex.org/W2726542547, https://openalex.org/W2025336185, https://openalex.org/W2586629973, https://openalex.org/W223909586, https://openalex.org/W3217485783, https://openalex.org/W2797542577, https://openalex.org/W2792809545, https://openalex.org/W2614443510, https://openalex.org/W2924124844, https://openalex.org/W2981317130, https://openalex.org/W3123723741, https://openalex.org/W2609243345, https://openalex.org/W2291298003, https://openalex.org/W2900082702, https://openalex.org/W2956015063, https://openalex.org/W2613362156, https://openalex.org/W2168133698, https://openalex.org/W2760673044, https://openalex.org/W2155943701, https://openalex.org/W2060965924, https://openalex.org/W2122732537, https://openalex.org/W2171777347, https://openalex.org/W2117131162, https://openalex.org/W2895452915, https://openalex.org/W2405513305, https://openalex.org/W2884903585, https://openalex.org/W2515462796, https://openalex.org/W2170490448, https://openalex.org/W2154974159, https://openalex.org/W2797282777, https://openalex.org/W2999309192, https://openalex.org/W2909679049, https://openalex.org/W2885727518 |
| referenced_works_count | 33 |
| abstract_inverted_index.a | 6, 36, 46, 64, 162 |
| abstract_inverted_index.We | 44 |
| abstract_inverted_index.an | 108, 129 |
| abstract_inverted_index.as | 63, 92, 94 |
| abstract_inverted_index.at | 35 |
| abstract_inverted_index.by | 71 |
| abstract_inverted_index.in | 15, 39, 170 |
| abstract_inverted_index.it | 32 |
| abstract_inverted_index.of | 18, 22, 30, 111, 125 |
| abstract_inverted_index.on | 83 |
| abstract_inverted_index.so | 34 |
| abstract_inverted_index.to | 9, 61, 67, 107, 132, 141, 165 |
| abstract_inverted_index.we | 86 |
| abstract_inverted_index.DNA | 52, 89, 158 |
| abstract_inverted_index.ITH | 73 |
| abstract_inverted_index.Our | 153 |
| abstract_inverted_index.The | 137 |
| abstract_inverted_index.and | 41, 122 |
| abstract_inverted_index.are | 58, 161 |
| abstract_inverted_index.can | 26 |
| abstract_inverted_index.few | 93 |
| abstract_inverted_index.for | 12, 79 |
| abstract_inverted_index.the | 16, 28 |
| abstract_inverted_index.two | 95 |
| abstract_inverted_index.ITH, | 31 |
| abstract_inverted_index.This | 105 |
| abstract_inverted_index.bona | 133 |
| abstract_inverted_index.cost | 40 |
| abstract_inverted_index.data | 145 |
| abstract_inverted_index.does | 33 |
| abstract_inverted_index.fide | 134 |
| abstract_inverted_index.from | 54, 91 |
| abstract_inverted_index.lung | 143 |
| abstract_inverted_index.risk | 120 |
| abstract_inverted_index.same | 138 |
| abstract_inverted_index.that | 88, 156 |
| abstract_inverted_index.with | 114, 128 |
| abstract_inverted_index.(ITH) | 4 |
| abstract_inverted_index.(TMB) | 151 |
| abstract_inverted_index.boost | 68 |
| abstract_inverted_index.leads | 106 |
| abstract_inverted_index.mixed | 59 |
| abstract_inverted_index.poses | 5 |
| abstract_inverted_index.prior | 60 |
| abstract_inverted_index.tumor | 24, 56, 148 |
| abstract_inverted_index.value | 70 |
| abstract_inverted_index.while | 74, 101 |
| abstract_inverted_index.burden | 150 |
| abstract_inverted_index.cancer | 144 |
| abstract_inverted_index.kidney | 84 |
| abstract_inverted_index.making | 14 |
| abstract_inverted_index.pooled | 47, 157 |
| abstract_inverted_index.tissue | 78 |
| abstract_inverted_index.address | 27, 166 |
| abstract_inverted_index.applied | 140 |
| abstract_inverted_index.cancer, | 85 |
| abstract_inverted_index.cancer. | 19 |
| abstract_inverted_index.genetic | 2, 168 |
| abstract_inverted_index.patient | 119 |
| abstract_inverted_index.pooling | 90 |
| abstract_inverted_index.propose | 45 |
| abstract_inverted_index.regions | 25, 57, 96 |
| abstract_inverted_index.whereby | 51 |
| abstract_inverted_index.ABSTRACT | 0 |
| abstract_inverted_index.Although | 20 |
| abstract_inverted_index.Focusing | 82 |
| abstract_inverted_index.accuracy | 130 |
| abstract_inverted_index.aliquots | 53 |
| abstract_inverted_index.approach | 139 |
| abstract_inverted_index.clinical | 171 |
| abstract_inverted_index.decision | 13 |
| abstract_inverted_index.findings | 154 |
| abstract_inverted_index.fraction | 110 |
| abstract_inverted_index.improved | 118, 123 |
| abstract_inverted_index.improves | 147 |
| abstract_inverted_index.increase | 38 |
| abstract_inverted_index.multiple | 23, 55 |
| abstract_inverted_index.mutation | 99 |
| abstract_inverted_index.patients | 112 |
| abstract_inverted_index.pitfalls | 29 |
| abstract_inverted_index.reducing | 102 |
| abstract_inverted_index.residual | 77 |
| abstract_inverted_index.resource | 42 |
| abstract_inverted_index.strategy | 66 |
| abstract_inverted_index.valuable | 76 |
| abstract_inverted_index.analysis. | 81 |
| abstract_inverted_index.challenge | 8 |
| abstract_inverted_index.clonality | 103 |
| abstract_inverted_index.detection | 100 |
| abstract_inverted_index.increased | 109 |
| abstract_inverted_index.increases | 98 |
| abstract_inverted_index.inference | 124 |
| abstract_inverted_index.intrinsic | 167 |
| abstract_inverted_index.secondary | 80 |
| abstract_inverted_index.settings. | 172 |
| abstract_inverted_index.strategy, | 50 |
| abstract_inverted_index.utilizing | 10 |
| abstract_inverted_index.actionable | 116 |
| abstract_inverted_index.addressing | 72 |
| abstract_inverted_index.comparable | 131 |
| abstract_inverted_index.detection. | 152 |
| abstract_inverted_index.identified | 113 |
| abstract_inverted_index.management | 17 |
| abstract_inverted_index.mutational | 149 |
| abstract_inverted_index.mutations, | 117 |
| abstract_inverted_index.preserving | 75 |
| abstract_inverted_index.sequencing | 11, 21, 49, 159 |
| abstract_inverted_index.strategies | 160 |
| abstract_inverted_index.alternative | 164 |
| abstract_inverted_index.demonstrate | 87, 155 |
| abstract_inverted_index.sequencing, | 62 |
| abstract_inverted_index.sequencing. | 136 |
| abstract_inverted_index.significant | 7, 37 |
| abstract_inverted_index.Intratumoral | 1 |
| abstract_inverted_index.evolutionary | 126 |
| abstract_inverted_index.trajectories | 127 |
| abstract_inverted_index.utilization. | 43 |
| abstract_inverted_index.heterogeneity | 3, 169 |
| abstract_inverted_index.multiregional | 48, 135 |
| abstract_inverted_index.significantly | 97 |
| abstract_inverted_index.substantially | 146 |
| abstract_inverted_index.translational | 69 |
| abstract_inverted_index.cost-effective | 65, 163 |
| abstract_inverted_index.non-small-cell | 142 |
| abstract_inverted_index.misattribution. | 104 |
| abstract_inverted_index.stratification, | 121 |
| abstract_inverted_index.therapeutically | 115 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5081768872, https://openalex.org/A5089094298 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 18 |
| corresponding_institution_ids | https://openalex.org/I1334819555, https://openalex.org/I4210150208 |
| citation_normalized_percentile.value | 0.05870761 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |