Review of phenotypic assays for detection of extended-spectrum β-lactamases and carbapenemases: a microbiology laboratory bench guide Article Swipe
Background: Infections caused by gram-negative antibiotic-resistant bacteria continue to increase. Despite recommenda- tions by the Clinical Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) with regards to detection of antibiotic degrading enzymes secreted by these bacteria, the true prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase producers remains a difficult task to resolve. Describing of previously designed phenotypic detection assays for ESBLs and carbapenemases in a single document avails a summary that allows for multiple testing which increases the sensitivity and specificity of detection. Methods and aims: This review, therefore, defined and classified ESBLs and carbapenemases, and also briefly described how the several previously designed phenotypic detection assays for the same should be performed. Conclusion: Extended-spectrum β-lactamase and carbapenemase detection assays, once performed correctly, can precisely discriminate between bacteria producing these enzymes and those with other mechanisms of resistance to β-lactam antibi- otics. Keywords: Extended-spectrum β-lactamases; carbapenemases; phenotypic detection.
Related Topics
- Type
- review
- Language
- en
- Landing Page
- https://doi.org/10.4314/ahs.v20i3.11
- https://www.ajol.info/index.php/ahs/article/download/200298/188890
- OA Status
- diamond
- Cited By
- 13
- References
- 94
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3092638266
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3092638266Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.4314/ahs.v20i3.11Digital Object Identifier
- Title
-
Review of phenotypic assays for detection of extended-spectrum β-lactamases and carbapenemases: a microbiology laboratory bench guideWork title
- Type
-
reviewOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-10-07Full publication date if available
- Authors
-
Dickson AruhomukamaList of authors in order
- Landing page
-
https://doi.org/10.4314/ahs.v20i3.11Publisher landing page
- PDF URL
-
https://www.ajol.info/index.php/ahs/article/download/200298/188890Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
diamondOpen access status per OpenAlex
- OA URL
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https://www.ajol.info/index.php/ahs/article/download/200298/188890Direct OA link when available
- Concepts
-
Clinical microbiology, Microbiology, Antibiotics, Bacteria, Antibiotic resistance, Medicine, Antimicrobial, Phenotype, Computational biology, Biology, Genetics, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
13Total citation count in OpenAlex
- Citations by year (recent)
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2025: 6, 2024: 1, 2023: 2, 2022: 1, 2021: 2Per-year citation counts (last 5 years)
- References (count)
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94Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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