RNA splicing and alternative polyadenylation profile during sheep zygotic genome activation Article Swipe
Mingtian Deng
,
Hua Yang
,
Ying Chen
,
Nasser Ghanem
,
Yingnan Yang
,
Xiaowei Chen
,
Jinhao Zhang
,
Feng Wang
,
Liqin Wang
,
Yanli Zhang
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/biolre/ioaf068
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/biolre/ioaf068
Zygotic genome activation (ZGA) is a critical biological step in mammalian early embryo development. However, ZGA initiation in sheep and the related sophisticated RNA metabolism remains largely unknown. Here, we observed extensive alterations in gene expression and DNA methylation patterns, along with elevated levels of RNA polymerase II (RNAPII) and its phosphorylation at serine 2 (RNAPII-Ser2P) at the 16-cell stage. Moreover, the embryos were blocked at the 16-cell stage embryo when treated with α-Amanitin, indicating that ZGA is initiated at the 16-cell stage in sheep in vitro fertilized embryos. To uncover the sophisticated RNA metabolism during ZGA, we conducted weighted gene co-expression network analysis and identified 1957 critical maternal genes, including TET3, UHRF1, and KIF2C. Using dapars analysis, we discovered 1058 and 933 lengthened alternative polyadenylation (APA) events during ZGA in sheep and mice. Specifically, genes exhibiting shorten APA were highly expressed at sheep 16-cell stage embryos and mouse 2-cell stage embryos. During ZGA in sheep and mice, 2675 and 1963 genes showed exon skipping, an alternative splicing (AS) events, which is related to RNA binding, translation, gamete generation, and reproduction. Of note, inhibition of AS led to 2-cell block in mice and 8/16-cell block in sheep. Moreover, 5-EU, RNAPII, and RNAPII-Ser2P signal were decreased in AS inhibited 2-cell embryos in mice, suggesting AS might regulate the ZGA process by crosstalk with RNAPII. In conclusion, our data confirmed ZGA initiation at the 16-cell stage embryos, and provides insights into the complex RNA metabolism during ZGA in mammals.
Related Topics
Concepts
Biology
Maternal to zygotic transition
Polyadenylation
Cell biology
Embryo
RNA
Alternative splicing
RNA splicing
Gene
Exon
Molecular biology
Gene expression
Genetics
Embryogenesis
Zygote
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1093/biolre/ioaf068
- https://academic.oup.com/biolreprod/advance-article-pdf/doi/10.1093/biolre/ioaf068/62801100/ioaf068.pdf
- OA Status
- bronze
- Cited By
- 2
- References
- 47
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4408997523
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4408997523Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1093/biolre/ioaf068Digital Object Identifier
- Title
-
RNA splicing and alternative polyadenylation profile during sheep zygotic genome activationWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
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2025Year of publication
- Publication date
-
2025-03-28Full publication date if available
- Authors
-
Mingtian Deng, Hua Yang, Ying Chen, Nasser Ghanem, Yingnan Yang, Xiaowei Chen, Jinhao Zhang, Feng Wang, Liqin Wang, Yanli ZhangList of authors in order
- Landing page
-
https://doi.org/10.1093/biolre/ioaf068Publisher landing page
- PDF URL
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https://academic.oup.com/biolreprod/advance-article-pdf/doi/10.1093/biolre/ioaf068/62801100/ioaf068.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
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bronzeOpen access status per OpenAlex
- OA URL
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https://academic.oup.com/biolreprod/advance-article-pdf/doi/10.1093/biolre/ioaf068/62801100/ioaf068.pdfDirect OA link when available
- Concepts
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Biology, Maternal to zygotic transition, Polyadenylation, Cell biology, Embryo, RNA, Alternative splicing, RNA splicing, Gene, Exon, Molecular biology, Gene expression, Genetics, Embryogenesis, ZygoteTop concepts (fields/topics) attached by OpenAlex
- Cited by
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2Total citation count in OpenAlex
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2025: 2Per-year citation counts (last 5 years)
- References (count)
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47Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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