Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time Sequencing Article Swipe
Fereidoun Forghani
,
Shaoting Li
,
Shaokang Zhang
,
David A. Mann
,
Xiangyu Deng
,
Henk C. den Bakker
,
Francisco Diez‐Gonzalez
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1128/aem.00097-20
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1128/aem.00097-20
Enterohemorrhagic Escherichia coli (EHEC) and Salmonella are of serious concern in low-moisture foods, including wheat flour and its related products, causing illnesses, outbreaks, and recalls. The development of advanced detection methods based on molecular principles of analysis is essential to incorporate into interventions intended to reduce the risk from these pathogens. In this work, a quasimetagenomic method based on real-time sequencing analysis and assisted by magnetic capture and DNA amplification was developed. This protocol is capable of detecting multiple Salmonella and/or E. coli organisms in the sample within less than a day, and it can also generate sufficient whole-genome sequences of the target organisms suitable for subsequent bioinformatics analysis. Multiplex detection and identification were accomplished in less than 20 h and additional whole-genome analyses of different nature were attained within 36 h, in contrast to the several days required in previous sequencing pipelines.
Related Topics
Concepts
Salmonella enterica
Escherichia coli
Salmonella
Biology
Multiplex
Serotype
Multiple displacement amplification
Genome
Computational biology
DNA sequencing
Microbiology
Polymerase chain reaction
Genetics
Bacteria
DNA
Gene
DNA extraction
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1128/aem.00097-20
- OA Status
- green
- Cited By
- 19
- References
- 44
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3021587118
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3021587118Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1128/aem.00097-20Digital Object Identifier
- Title
-
Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time SequencingWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-04-29Full publication date if available
- Authors
-
Fereidoun Forghani, Shaoting Li, Shaokang Zhang, David A. Mann, Xiangyu Deng, Henk C. den Bakker, Francisco Diez‐GonzalezList of authors in order
- Landing page
-
https://doi.org/10.1128/aem.00097-20Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://www.ncbi.nlm.nih.gov/pmc/articles/7301854Direct OA link when available
- Concepts
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Salmonella enterica, Escherichia coli, Salmonella, Biology, Multiplex, Serotype, Multiple displacement amplification, Genome, Computational biology, DNA sequencing, Microbiology, Polymerase chain reaction, Genetics, Bacteria, DNA, Gene, DNA extractionTop concepts (fields/topics) attached by OpenAlex
- Cited by
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19Total citation count in OpenAlex
- Citations by year (recent)
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2025: 4, 2023: 2, 2022: 8, 2021: 4, 2019: 1Per-year citation counts (last 5 years)
- References (count)
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44Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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