SangeR: The high-throughput Sanger sequenc-ing analysis pipeline Article Swipe

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Pipeline (software) Sanger sequencing Throughput Computer science Computational biology Biology Genetics Gene DNA sequencing Telecommunications Wireless Programming language

Kai Schmid , Hildegard Dohmen , Nadja Ritschel , Jochen Zohner , Carmen Selignow , Jannik Sehring , Till Acker , Daniel Amsel ·

YOU? · · 2021 · Open Access · · DOI: https://doi.org/10.5281/zenodo.5865470 · OA: W4287112412

<strong>Summary:</strong> In the era of Next Generation Sequencing and beyond, the Sanger technique is still widely used for variant verification tasks of ambiguous high-throughput sequencing results or as cheap molecular genetical analysis of singular targets in many fields of study. Nevertheless, many analysis steps need time consuming manual intervention. Therefore, we here present a pipeline-able high-throughput solution with optional R-Shiny web-interface, delivering a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files.