SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease Article Swipe

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Raphael A. Reyes , Kathleen Clarke , S. Jake Gonzales , Angelene M. Cantwell , Rolando Garza , Gabriel Catano , Robin Tragus , Thomas F. Patterson , Sebastiaan Bol , Evelien M. Bunnik ·

YOU? · · 2021 · Open Access · · DOI: https://doi.org/10.1101/2021.09.24.461732

SARS-CoV-2 infection elicits a robust B cell response, resulting in the generation of long-lived plasma cells and memory B cells. Here, we aimed to determine the effect of COVID-19 severity on the memory B cell response and characterize changes in the memory B cell compartment between recovery and five months post-symptom onset. Using high-parameter spectral flow cytometry, we analyzed the phenotype of memory B cells with reactivity against the SARS-CoV-2 spike protein or the spike receptor binding domain (RBD) in recovered individuals who had been hospitalized with non-severe (n=8) or severe (n=5) COVID-19. One month after symptom onset, a substantial proportion of spike-specific IgG + B cells showed an activated phenotype. In individuals who experienced non-severe disease, spike-specific IgG + B cells showed increased expression of markers associated with durable B cell memory, including T-bet, FcRL5, and CD11c, which was not observed after severe disease. Five months post-symptom onset, the majority of spike-specific memory B cells had a resting phenotype and the percentage of spike-specific T-bet + IgG + memory B cells decreased to baseline levels. Collectively, our results suggest that the memory B cell response elicited during non-severe COVID-19 may be of higher quality than the response after severe disease.

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Memory B cell Flow cytometry Immunology Phenotype CD11c Memory cell Disease Biology B cell Medicine Antibody Internal medicine Genetics Gene Physics Voltage Quantum mechanics Transistor

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1101/2021.09.24.461732
PDF: https://www.biorxiv.org/content/biorxiv/early/2021/09/27/2021.09.24.461732.full.pdf
OA Status: green
Cited By: 6
References: 51
Related Works: 10
OpenAlex ID: https://openalex.org/W3202805413

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DOI: https://doi.org/10.1101/2021.09.24.461732

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Title: SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease

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Type: preprint

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Language: en

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Publication year: 2021

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Publication date: 2021-09-27

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Authors: Raphael A. Reyes, Kathleen Clarke, S. Jake Gonzales, Angelene M. Cantwell, Rolando Garza, Gabriel Catano, Robin Tragus, Thomas F. Patterson, Sebastiaan Bol, Evelien M. Bunnik

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Landing page: https://doi.org/10.1101/2021.09.24.461732

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PDF URL: https://www.biorxiv.org/content/biorxiv/early/2021/09/27/2021.09.24.461732.full.pdf

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OA URL: https://www.biorxiv.org/content/biorxiv/early/2021/09/27/2021.09.24.461732.full.pdf

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Concepts: Memory B cell, Flow cytometry, Immunology, Phenotype, CD11c, Memory cell, Disease, Biology, B cell, Medicine, Antibody, Internal medicine, Genetics, Gene, Physics, Voltage, Quantum mechanics, Transistor

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Cited by: 6

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Citations by year (recent): 2024: 1, 2022: 4, 2021: 1

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References (count): 51

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Related works (count): 10

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