Self-organization of actin networks by a monomeric myosin Article Swipe
YOU?
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· 2016
· Open Access
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· DOI: https://doi.org/10.1073/pnas.1612719113
· OA: W2561114851
Significance Actomyosin networks are central to a broad range of cellular motile processes, including cell polarization and collective cell migration during morphogenesis and development. Myosin-IXa is critically involved in these processes. Using fluorescence spectroscopy, total internal reflection fluorescence, and electron microscopy, we demonstrate that myosin-IXa assembles actin filaments into highly ordered lattices. The actin filaments of parallel polarity are connected by myosin-IXa in distinct conformations and at a repeat distance of 36 nm across the network. The myosin-IXa–induced actin lattices introduce orientated actin tracks and a network of regularly spaced platforms for localized Rho-GTPase–activating protein activity in cell polarization and collective cell migration.
