SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli Article Swipe
Adrian J. Jervis
,
Pablo Carbonell
,
Sandra Taylor
,
Rehana Sung
,
Mark S. Dunstan
,
Christopher Robinson
,
Rainer Breitling
,
Eriko Takano
,
Nigel S. Scrutton
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1021/acssynbio.8b00399
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1021/acssynbio.8b00399
The rapid prototyping and optimization of plasmid-based recombinant gene expression is one of the key steps in the development of bioengineered bacterial systems. Often, multiple genes or gene modules need to be coexpressed, and for this purpose compatible, inducible plasmid systems have been developed. However, inducible expression systems are not favored in industrial processes, due to their prohibitive cost, and consequently the conversion to constitutive expression systems is often desired. Here we present a set of constitutive-expression plasmids for this purpose, which were benchmarked using fluorescent reporter genes. To further facilitate the conversion between inducible and constitutive expression systems, we developed SelProm, a design tool that serves as a parts repository of plasmid expression strength and predicts portability rules between constitutive and inducible plasmids through model comparison and machine learning. The SelProm tool is freely available at http://selprom.synbiochem.co.uk .
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acssynbio.8b00399
- OA Status
- hybrid
- Cited By
- 43
- References
- 14
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2921412988
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2921412988Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1021/acssynbio.8b00399Digital Object Identifier
- Title
-
SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coliWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2019Year of publication
- Publication date
-
2019-03-14Full publication date if available
- Authors
-
Adrian J. Jervis, Pablo Carbonell, Sandra Taylor, Rehana Sung, Mark S. Dunstan, Christopher Robinson, Rainer Breitling, Eriko Takano, Nigel S. ScruttonList of authors in order
- Landing page
-
https://doi.org/10.1021/acssynbio.8b00399Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1021/acssynbio.8b00399Direct OA link when available
- Concepts
-
Plasmid, Software portability, Computational biology, Escherichia coli, Expression vector, Reporter gene, Biology, Gene, Expression (computer science), Gene expression, Computer science, Recombinant DNA, Genetics, Programming languageTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
43Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 2, 2024: 5, 2023: 4, 2022: 4, 2021: 14Per-year citation counts (last 5 years)
- References (count)
-
14Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2921412988 |
|---|---|
| doi | https://doi.org/10.1021/acssynbio.8b00399 |
| ids.doi | https://doi.org/10.1021/acssynbio.8b00399 |
| ids.mag | 2921412988 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/30870592 |
| ids.openalex | https://openalex.org/W2921412988 |
| fwci | 3.29763225 |
| mesh[0].qualifier_ui | Q000235 |
| mesh[0].descriptor_ui | D004926 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | genetics |
| mesh[0].descriptor_name | Escherichia coli |
| mesh[1].qualifier_ui | |
| mesh[1].descriptor_ui | D005453 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | |
| mesh[1].descriptor_name | Fluorescence |
| mesh[2].qualifier_ui | Q000235 |
| mesh[2].descriptor_ui | D015870 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | genetics |
| mesh[2].descriptor_name | Gene Expression |
| mesh[3].qualifier_ui | Q000235 |
| mesh[3].descriptor_ui | D015964 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | genetics |
| mesh[3].descriptor_name | Gene Expression Regulation, Bacterial |
| mesh[4].qualifier_ui | Q000235 |
| mesh[4].descriptor_ui | D017930 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | genetics |
| mesh[4].descriptor_name | Genes, Reporter |
| mesh[5].qualifier_ui | Q000235 |
| mesh[5].descriptor_ui | D005822 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | genetics |
| mesh[5].descriptor_name | Genetic Vectors |
| mesh[6].qualifier_ui | Q000235 |
| mesh[6].descriptor_ui | D010957 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | genetics |
| mesh[6].descriptor_name | Plasmids |
| mesh[7].qualifier_ui | Q000235 |
| mesh[7].descriptor_ui | D004926 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | genetics |
| mesh[7].descriptor_name | Escherichia coli |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D005453 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Fluorescence |
| mesh[9].qualifier_ui | Q000235 |
| mesh[9].descriptor_ui | D015870 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | genetics |
| mesh[9].descriptor_name | Gene Expression |
| mesh[10].qualifier_ui | Q000235 |
| mesh[10].descriptor_ui | D015964 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | genetics |
| mesh[10].descriptor_name | Gene Expression Regulation, Bacterial |
| mesh[11].qualifier_ui | Q000235 |
| mesh[11].descriptor_ui | D017930 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | genetics |
| mesh[11].descriptor_name | Genes, Reporter |
| mesh[12].qualifier_ui | Q000235 |
| mesh[12].descriptor_ui | D005822 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | genetics |
| mesh[12].descriptor_name | Genetic Vectors |
| mesh[13].qualifier_ui | Q000235 |
| mesh[13].descriptor_ui | D010957 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | genetics |
| mesh[13].descriptor_name | Plasmids |
| mesh[14].qualifier_ui | Q000235 |
| mesh[14].descriptor_ui | D004926 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | genetics |
| mesh[14].descriptor_name | Escherichia coli |
| mesh[15].qualifier_ui | |
| mesh[15].descriptor_ui | D005453 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | |
| mesh[15].descriptor_name | Fluorescence |
| mesh[16].qualifier_ui | Q000235 |
| mesh[16].descriptor_ui | D015870 |
| mesh[16].is_major_topic | False |
| mesh[16].qualifier_name | genetics |
| mesh[16].descriptor_name | Gene Expression |
| mesh[17].qualifier_ui | Q000235 |
| mesh[17].descriptor_ui | D015964 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | genetics |
| mesh[17].descriptor_name | Gene Expression Regulation, Bacterial |
| mesh[18].qualifier_ui | Q000235 |
| mesh[18].descriptor_ui | D017930 |
| mesh[18].is_major_topic | False |
| mesh[18].qualifier_name | genetics |
| mesh[18].descriptor_name | Genes, Reporter |
| mesh[19].qualifier_ui | Q000235 |
| mesh[19].descriptor_ui | D005822 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | genetics |
| mesh[19].descriptor_name | Genetic Vectors |
| mesh[20].qualifier_ui | Q000235 |
| mesh[20].descriptor_ui | D010957 |
| mesh[20].is_major_topic | False |
| mesh[20].qualifier_name | genetics |
| mesh[20].descriptor_name | Plasmids |
| type | article |
| title | SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli |
| awards[0].id | https://openalex.org/G2101378041 |
| awards[0].funder_id | https://openalex.org/F4320334627 |
| awards[0].display_name | |
| awards[0].funder_award_id | EP/J020192/1 |
| awards[0].funder_display_name | Engineering and Physical Sciences Research Council |
| awards[1].id | https://openalex.org/G2243314598 |
| awards[1].funder_id | https://openalex.org/F4320334629 |
| awards[1].display_name | |
| awards[1].funder_award_id | BB/M017702/1 |
| awards[1].funder_display_name | Biotechnology and Biological Sciences Research Council |
| awards[2].id | https://openalex.org/G7135410833 |
| awards[2].funder_id | https://openalex.org/F4320334627 |
| awards[2].display_name | |
| awards[2].funder_award_id | EP/S01778X/1 |
| awards[2].funder_display_name | Engineering and Physical Sciences Research Council |
| awards[3].id | https://openalex.org/G3907908503 |
| awards[3].funder_id | https://openalex.org/F4320334629 |
| awards[3].display_name | |
| awards[3].funder_award_id | BB/R506497/1 |
| awards[3].funder_display_name | Biotechnology and Biological Sciences Research Council |
| biblio.issue | 7 |
| biblio.volume | 8 |
| biblio.last_page | 1483 |
| biblio.first_page | 1478 |
| topics[0].id | https://openalex.org/T11663 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9995999932289124 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1312 |
| topics[0].subfield.display_name | Molecular Biology |
| topics[0].display_name | Viral Infectious Diseases and Gene Expression in Insects |
| topics[1].id | https://openalex.org/T10932 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9990000128746033 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Microbial Metabolic Engineering and Bioproduction |
| topics[2].id | https://openalex.org/T10120 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9914000034332275 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1311 |
| topics[2].subfield.display_name | Genetics |
| topics[2].display_name | Bacterial Genetics and Biotechnology |
| funders[0].id | https://openalex.org/F4320320006 |
| funders[0].ror | https://ror.org/03wnrjx87 |
| funders[0].display_name | Royal Society |
| funders[1].id | https://openalex.org/F4320320670 |
| funders[1].ror | https://ror.org/0333xzh65 |
| funders[1].display_name | Wolfson Foundation |
| funders[2].id | https://openalex.org/F4320334627 |
| funders[2].ror | https://ror.org/0439y7842 |
| funders[2].display_name | Engineering and Physical Sciences Research Council |
| funders[3].id | https://openalex.org/F4320334629 |
| funders[3].ror | https://ror.org/00cwqg982 |
| funders[3].display_name | Biotechnology and Biological Sciences Research Council |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C22744801 |
| concepts[0].level | 3 |
| concepts[0].score | 0.8418808579444885 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q172778 |
| concepts[0].display_name | Plasmid |
| concepts[1].id | https://openalex.org/C63000827 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6834467053413391 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q3080428 |
| concepts[1].display_name | Software portability |
| concepts[2].id | https://openalex.org/C70721500 |
| concepts[2].level | 1 |
| concepts[2].score | 0.5475354194641113 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[2].display_name | Computational biology |
| concepts[3].id | https://openalex.org/C547475151 |
| concepts[3].level | 3 |
| concepts[3].score | 0.5475335121154785 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q25419 |
| concepts[3].display_name | Escherichia coli |
| concepts[4].id | https://openalex.org/C33161422 |
| concepts[4].level | 4 |
| concepts[4].score | 0.5309877991676331 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q5421712 |
| concepts[4].display_name | Expression vector |
| concepts[5].id | https://openalex.org/C161733203 |
| concepts[5].level | 4 |
| concepts[5].score | 0.5302514433860779 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q911828 |
| concepts[5].display_name | Reporter gene |
| concepts[6].id | https://openalex.org/C86803240 |
| concepts[6].level | 0 |
| concepts[6].score | 0.5273309946060181 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[6].display_name | Biology |
| concepts[7].id | https://openalex.org/C104317684 |
| concepts[7].level | 2 |
| concepts[7].score | 0.47042369842529297 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[7].display_name | Gene |
| concepts[8].id | https://openalex.org/C90559484 |
| concepts[8].level | 2 |
| concepts[8].score | 0.44008782505989075 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q778379 |
| concepts[8].display_name | Expression (computer science) |
| concepts[9].id | https://openalex.org/C150194340 |
| concepts[9].level | 3 |
| concepts[9].score | 0.4092192053794861 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q26972 |
| concepts[9].display_name | Gene expression |
| concepts[10].id | https://openalex.org/C41008148 |
| concepts[10].level | 0 |
| concepts[10].score | 0.3870912790298462 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q21198 |
| concepts[10].display_name | Computer science |
| concepts[11].id | https://openalex.org/C40767141 |
| concepts[11].level | 3 |
| concepts[11].score | 0.3011006712913513 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q285697 |
| concepts[11].display_name | Recombinant DNA |
| concepts[12].id | https://openalex.org/C54355233 |
| concepts[12].level | 1 |
| concepts[12].score | 0.288607656955719 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[12].display_name | Genetics |
| concepts[13].id | https://openalex.org/C199360897 |
| concepts[13].level | 1 |
| concepts[13].score | 0.08675894141197205 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q9143 |
| concepts[13].display_name | Programming language |
| keywords[0].id | https://openalex.org/keywords/plasmid |
| keywords[0].score | 0.8418808579444885 |
| keywords[0].display_name | Plasmid |
| keywords[1].id | https://openalex.org/keywords/software-portability |
| keywords[1].score | 0.6834467053413391 |
| keywords[1].display_name | Software portability |
| keywords[2].id | https://openalex.org/keywords/computational-biology |
| keywords[2].score | 0.5475354194641113 |
| keywords[2].display_name | Computational biology |
| keywords[3].id | https://openalex.org/keywords/escherichia-coli |
| keywords[3].score | 0.5475335121154785 |
| keywords[3].display_name | Escherichia coli |
| keywords[4].id | https://openalex.org/keywords/expression-vector |
| keywords[4].score | 0.5309877991676331 |
| keywords[4].display_name | Expression vector |
| keywords[5].id | https://openalex.org/keywords/reporter-gene |
| keywords[5].score | 0.5302514433860779 |
| keywords[5].display_name | Reporter gene |
| keywords[6].id | https://openalex.org/keywords/biology |
| keywords[6].score | 0.5273309946060181 |
| keywords[6].display_name | Biology |
| keywords[7].id | https://openalex.org/keywords/gene |
| keywords[7].score | 0.47042369842529297 |
| keywords[7].display_name | Gene |
| keywords[8].id | https://openalex.org/keywords/expression |
| keywords[8].score | 0.44008782505989075 |
| keywords[8].display_name | Expression (computer science) |
| keywords[9].id | https://openalex.org/keywords/gene-expression |
| keywords[9].score | 0.4092192053794861 |
| keywords[9].display_name | Gene expression |
| keywords[10].id | https://openalex.org/keywords/computer-science |
| keywords[10].score | 0.3870912790298462 |
| keywords[10].display_name | Computer science |
| keywords[11].id | https://openalex.org/keywords/recombinant-dna |
| keywords[11].score | 0.3011006712913513 |
| keywords[11].display_name | Recombinant DNA |
| keywords[12].id | https://openalex.org/keywords/genetics |
| keywords[12].score | 0.288607656955719 |
| keywords[12].display_name | Genetics |
| keywords[13].id | https://openalex.org/keywords/programming-language |
| keywords[13].score | 0.08675894141197205 |
| keywords[13].display_name | Programming language |
| language | en |
| locations[0].id | doi:10.1021/acssynbio.8b00399 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S54167817 |
| locations[0].source.issn | 2161-5063 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 2161-5063 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | ACS Synthetic Biology |
| locations[0].source.host_organization | https://openalex.org/P4310320006 |
| locations[0].source.host_organization_name | American Chemical Society |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320006 |
| locations[0].source.host_organization_lineage_names | American Chemical Society |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | ACS Synthetic Biology |
| locations[0].landing_page_url | https://doi.org/10.1021/acssynbio.8b00399 |
| locations[1].id | pmid:30870592 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | ACS synthetic biology |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/30870592 |
| locations[2].id | pmh:oai:pure.atira.dk:openaire_cris_publications/8a9acaae-0386-49ff-a10e-993b86a14eed |
| locations[2].is_oa | False |
| locations[2].source.id | https://openalex.org/S4306400216 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | Research Portal (King's College London) |
| locations[2].source.host_organization | https://openalex.org/I183935753 |
| locations[2].source.host_organization_name | King's College London |
| locations[2].source.host_organization_lineage | https://openalex.org/I183935753 |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Jervis, A J, Carbonell, P, Taylor, S, Sung, R, Dunstan, M S, Robinson, C J, Breitling, R, Takano, E & Scrutton, N S 2019, 'SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli', ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00399 |
| locations[2].landing_page_url | https://research.manchester.ac.uk/en/publications/8a9acaae-0386-49ff-a10e-993b86a14eed |
| indexed_in | crossref, pubmed |
| authorships[0].author.id | https://openalex.org/A5007448810 |
| authorships[0].author.orcid | https://orcid.org/0000-0003-2386-3354 |
| authorships[0].author.display_name | Adrian J. Jervis |
| authorships[0].countries | GB |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[0].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[0].institutions[0].id | https://openalex.org/I28407311 |
| authorships[0].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[0].institutions[0].country_code | GB |
| authorships[0].institutions[0].display_name | University of Manchester |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Adrian J. Jervis |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[1].author.id | https://openalex.org/A5026535521 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-0993-5625 |
| authorships[1].author.display_name | Pablo Carbonell |
| authorships[1].countries | GB |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[1].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[1].institutions[0].id | https://openalex.org/I28407311 |
| authorships[1].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[1].institutions[0].country_code | GB |
| authorships[1].institutions[0].display_name | University of Manchester |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Pablo Carbonell |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[2].author.id | https://openalex.org/A5041028053 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-8807-5554 |
| authorships[2].author.display_name | Sandra Taylor |
| authorships[2].countries | GB |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[2].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[2].institutions[0].id | https://openalex.org/I28407311 |
| authorships[2].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[2].institutions[0].country_code | GB |
| authorships[2].institutions[0].display_name | University of Manchester |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Sandra Taylor |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[3].author.id | https://openalex.org/A5058159877 |
| authorships[3].author.orcid | https://orcid.org/0009-0006-9010-8497 |
| authorships[3].author.display_name | Rehana Sung |
| authorships[3].countries | GB |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[3].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[3].institutions[0].id | https://openalex.org/I28407311 |
| authorships[3].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[3].institutions[0].country_code | GB |
| authorships[3].institutions[0].display_name | University of Manchester |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Rehana Sung |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[4].author.id | https://openalex.org/A5060517464 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-9416-5505 |
| authorships[4].author.display_name | Mark S. Dunstan |
| authorships[4].countries | GB |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[4].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[4].institutions[0].id | https://openalex.org/I28407311 |
| authorships[4].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[4].institutions[0].country_code | GB |
| authorships[4].institutions[0].display_name | University of Manchester |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Mark S. Dunstan |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[5].author.id | https://openalex.org/A5052454334 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-6146-566X |
| authorships[5].author.display_name | Christopher Robinson |
| authorships[5].countries | GB |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[5].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[5].institutions[0].id | https://openalex.org/I28407311 |
| authorships[5].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[5].institutions[0].country_code | GB |
| authorships[5].institutions[0].display_name | University of Manchester |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Christopher J. Robinson |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[6].author.id | https://openalex.org/A5070145336 |
| authorships[6].author.orcid | https://orcid.org/0000-0001-7173-0922 |
| authorships[6].author.display_name | Rainer Breitling |
| authorships[6].countries | GB |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[6].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[6].institutions[0].id | https://openalex.org/I28407311 |
| authorships[6].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[6].institutions[0].country_code | GB |
| authorships[6].institutions[0].display_name | University of Manchester |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Rainer Breitling |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[7].author.id | https://openalex.org/A5066686497 |
| authorships[7].author.orcid | https://orcid.org/0000-0002-6791-3256 |
| authorships[7].author.display_name | Eriko Takano |
| authorships[7].countries | GB |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[7].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[7].institutions[0].id | https://openalex.org/I28407311 |
| authorships[7].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[7].institutions[0].country_code | GB |
| authorships[7].institutions[0].display_name | University of Manchester |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Eriko Takano |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[8].author.id | https://openalex.org/A5058612126 |
| authorships[8].author.orcid | https://orcid.org/0000-0002-4182-3500 |
| authorships[8].author.display_name | Nigel S. Scrutton |
| authorships[8].countries | GB |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I28407311 |
| authorships[8].affiliations[0].raw_affiliation_string | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| authorships[8].institutions[0].id | https://openalex.org/I28407311 |
| authorships[8].institutions[0].ror | https://ror.org/027m9bs27 |
| authorships[8].institutions[0].type | education |
| authorships[8].institutions[0].lineage | https://openalex.org/I28407311 |
| authorships[8].institutions[0].country_code | GB |
| authorships[8].institutions[0].display_name | University of Manchester |
| authorships[8].author_position | last |
| authorships[8].raw_author_name | Nigel S. Scrutton |
| authorships[8].is_corresponding | True |
| authorships[8].raw_affiliation_strings | Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology and School of Chemistry, University of Manchester, Manchester M1 7DN, U.K. |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.1021/acssynbio.8b00399 |
| open_access.oa_status | hybrid |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11663 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9995999932289124 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1312 |
| primary_topic.subfield.display_name | Molecular Biology |
| primary_topic.display_name | Viral Infectious Diseases and Gene Expression in Insects |
| related_works | https://openalex.org/W2408202374, https://openalex.org/W2408202795, https://openalex.org/W973393354, https://openalex.org/W2388438194, https://openalex.org/W2360457928, https://openalex.org/W2365509260, https://openalex.org/W2358774340, https://openalex.org/W2969666982, https://openalex.org/W2415399868, https://openalex.org/W2028219578 |
| cited_by_count | 43 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 2 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 5 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 4 |
| counts_by_year[3].year | 2022 |
| counts_by_year[3].cited_by_count | 4 |
| counts_by_year[4].year | 2021 |
| counts_by_year[4].cited_by_count | 14 |
| counts_by_year[5].year | 2020 |
| counts_by_year[5].cited_by_count | 12 |
| counts_by_year[6].year | 2019 |
| counts_by_year[6].cited_by_count | 2 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1021/acssynbio.8b00399 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S54167817 |
| best_oa_location.source.issn | 2161-5063 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | 2161-5063 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | ACS Synthetic Biology |
| best_oa_location.source.host_organization | https://openalex.org/P4310320006 |
| best_oa_location.source.host_organization_name | American Chemical Society |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| best_oa_location.source.host_organization_lineage_names | American Chemical Society |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | ACS Synthetic Biology |
| best_oa_location.landing_page_url | https://doi.org/10.1021/acssynbio.8b00399 |
| primary_location.id | doi:10.1021/acssynbio.8b00399 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S54167817 |
| primary_location.source.issn | 2161-5063 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 2161-5063 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | ACS Synthetic Biology |
| primary_location.source.host_organization | https://openalex.org/P4310320006 |
| primary_location.source.host_organization_name | American Chemical Society |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320006 |
| primary_location.source.host_organization_lineage_names | American Chemical Society |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | ACS Synthetic Biology |
| primary_location.landing_page_url | https://doi.org/10.1021/acssynbio.8b00399 |
| publication_date | 2019-03-14 |
| publication_year | 2019 |
| referenced_works | https://openalex.org/W2041618640, https://openalex.org/W1967382476, https://openalex.org/W2806394571, https://openalex.org/W2168059482, https://openalex.org/W2107523606, https://openalex.org/W1982228455, https://openalex.org/W2080233018, https://openalex.org/W2134783465, https://openalex.org/W2001426985, https://openalex.org/W1970422609, https://openalex.org/W2287820377, https://openalex.org/W2766283429, https://openalex.org/W2781033190, https://openalex.org/W2792353080 |
| referenced_works_count | 14 |
| abstract_inverted_index.. | 138 |
| abstract_inverted_index.a | 73, 102, 108 |
| abstract_inverted_index.To | 88 |
| abstract_inverted_index.as | 107 |
| abstract_inverted_index.at | 136 |
| abstract_inverted_index.be | 31 |
| abstract_inverted_index.in | 16, 51 |
| abstract_inverted_index.is | 10, 67, 133 |
| abstract_inverted_index.of | 5, 12, 19, 75, 111 |
| abstract_inverted_index.or | 26 |
| abstract_inverted_index.to | 30, 55, 63 |
| abstract_inverted_index.we | 71, 99 |
| abstract_inverted_index.The | 0, 130 |
| abstract_inverted_index.and | 3, 33, 59, 95, 115, 121, 127 |
| abstract_inverted_index.are | 48 |
| abstract_inverted_index.due | 54 |
| abstract_inverted_index.for | 34, 78 |
| abstract_inverted_index.key | 14 |
| abstract_inverted_index.not | 49 |
| abstract_inverted_index.one | 11 |
| abstract_inverted_index.set | 74 |
| abstract_inverted_index.the | 13, 17, 61, 91 |
| abstract_inverted_index.Here | 70 |
| abstract_inverted_index.been | 42 |
| abstract_inverted_index.gene | 8, 27 |
| abstract_inverted_index.have | 41 |
| abstract_inverted_index.need | 29 |
| abstract_inverted_index.that | 105 |
| abstract_inverted_index.this | 35, 79 |
| abstract_inverted_index.tool | 104, 132 |
| abstract_inverted_index.were | 82 |
| abstract_inverted_index.cost, | 58 |
| abstract_inverted_index.genes | 25 |
| abstract_inverted_index.model | 125 |
| abstract_inverted_index.often | 68 |
| abstract_inverted_index.parts | 109 |
| abstract_inverted_index.rapid | 1 |
| abstract_inverted_index.rules | 118 |
| abstract_inverted_index.steps | 15 |
| abstract_inverted_index.their | 56 |
| abstract_inverted_index.using | 84 |
| abstract_inverted_index.which | 81 |
| abstract_inverted_index.Often, | 23 |
| abstract_inverted_index.design | 103 |
| abstract_inverted_index.freely | 134 |
| abstract_inverted_index.genes. | 87 |
| abstract_inverted_index.serves | 106 |
| abstract_inverted_index.SelProm | 131 |
| abstract_inverted_index.between | 93, 119 |
| abstract_inverted_index.favored | 50 |
| abstract_inverted_index.further | 89 |
| abstract_inverted_index.machine | 128 |
| abstract_inverted_index.modules | 28 |
| abstract_inverted_index.plasmid | 39, 112 |
| abstract_inverted_index.present | 72 |
| abstract_inverted_index.purpose | 36 |
| abstract_inverted_index.systems | 40, 47, 66 |
| abstract_inverted_index.through | 124 |
| abstract_inverted_index.However, | 44 |
| abstract_inverted_index.SelProm, | 101 |
| abstract_inverted_index.desired. | 69 |
| abstract_inverted_index.multiple | 24 |
| abstract_inverted_index.plasmids | 77, 123 |
| abstract_inverted_index.predicts | 116 |
| abstract_inverted_index.purpose, | 80 |
| abstract_inverted_index.reporter | 86 |
| abstract_inverted_index.strength | 114 |
| abstract_inverted_index.systems, | 98 |
| abstract_inverted_index.systems. | 22 |
| abstract_inverted_index.available | 135 |
| abstract_inverted_index.bacterial | 21 |
| abstract_inverted_index.developed | 100 |
| abstract_inverted_index.inducible | 38, 45, 94, 122 |
| abstract_inverted_index.learning. | 129 |
| abstract_inverted_index.comparison | 126 |
| abstract_inverted_index.conversion | 62, 92 |
| abstract_inverted_index.developed. | 43 |
| abstract_inverted_index.expression | 9, 46, 65, 97, 113 |
| abstract_inverted_index.facilitate | 90 |
| abstract_inverted_index.industrial | 52 |
| abstract_inverted_index.processes, | 53 |
| abstract_inverted_index.repository | 110 |
| abstract_inverted_index.benchmarked | 83 |
| abstract_inverted_index.compatible, | 37 |
| abstract_inverted_index.development | 18 |
| abstract_inverted_index.fluorescent | 85 |
| abstract_inverted_index.portability | 117 |
| abstract_inverted_index.prohibitive | 57 |
| abstract_inverted_index.prototyping | 2 |
| abstract_inverted_index.recombinant | 7 |
| abstract_inverted_index.coexpressed, | 32 |
| abstract_inverted_index.consequently | 60 |
| abstract_inverted_index.constitutive | 64, 96, 120 |
| abstract_inverted_index.optimization | 4 |
| abstract_inverted_index.bioengineered | 20 |
| abstract_inverted_index.plasmid-based | 6 |
| abstract_inverted_index.constitutive-expression | 76 |
| abstract_inverted_index.http://selprom.synbiochem.co.uk | 137 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 94 |
| corresponding_author_ids | https://openalex.org/A5058612126 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 9 |
| corresponding_institution_ids | https://openalex.org/I28407311 |
| citation_normalized_percentile.value | 0.91525709 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |