Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering Article Swipe
Shouka Parvin Nejad
,
Monica Lecce
,
Bahram Mirani
,
Nataly Machado Siqueira
,
Zahra Mirzaei
,
J. Paul Santerre
,
John E. Davies
,
Craig A. Simmons
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-2219477/v1
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-2219477/v1
Background: Constructs currently used to repair or replace congenitally diseased pediatric heart valves lack a viable cell population capable of functional adaptation in situ , necessitating repeated surgical intervention. Heart valve tissue engineering (HVTE) can address these limitations by producing functional living tissue in vitro that holds the potential for somatic growth and remodelling upon implantation. However, clinical translation of HVTE strategies requires an appropriate source of autologous cells that can be non-invasively harvested from MSC-rich tissues and cultured under serum- and xeno-free conditions. To this end, we evaluated human umbilical cord perivascular cells (hUCPVCs) as a promising cell source for in vitro production of engineered heart valve tissue. Methods: The proliferative, clonogenic, multilineage differentiation, and extracellular matrix (ECM) synthesis capacities of hUCPVCs were evaluated in a commercial serum- and xeno-free culture medium (StemMACS™) on tissue culture polystyrene and benchmarked to adult bone marrow-derived MSCs (BMMSCs). Additionally, the ECM synthesis potential of hUCPVCs was evaluated when cultured on polycarbonate polyurethane anisotropic electrospun scaffolds, a representative biomaterial for in vitro HVTE. Results: hUCPVCs had greater proliferative and clonogenic potential than BMMSCs in StemMACS™ (p<0.05), without differentiation to osteogenic and adipogenic phenotypes associated with valve pathology. Furthermore, hUCPVCs cultured with StemMACS™ on tissue culture plastic for 14 days synthesized significantly more total collagen, elastin, and sulfated proteoglycans (p<0.05), the ECM constituents of the native valve, than BMMSCs. Finally, hUCPVCs retained their ECM synthesizing capacity after 14 and 21 days in culture on anisotropic electrospun scaffolds. Conclusion: Overall, our findings establish an in vitro culture platform that uses hUCPVCs as a readily-available and non-invasively sourced autologous cell population and a commercial serum- and xeno-free culture medium to increase the translational potential of future pediatric HVTE strategies.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-2219477/v1
- https://www.researchsquare.com/article/rs-2219477/latest.pdf
- OA Status
- green
- Cited By
- 1
- References
- 56
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4313464944
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4313464944Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-2219477/v1Digital Object Identifier
- Title
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Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineeringWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-01-03Full publication date if available
- Authors
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Shouka Parvin Nejad, Monica Lecce, Bahram Mirani, Nataly Machado Siqueira, Zahra Mirzaei, J. Paul Santerre, John E. Davies, Craig A. SimmonsList of authors in order
- Landing page
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https://doi.org/10.21203/rs.3.rs-2219477/v1Publisher landing page
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https://www.researchsquare.com/article/rs-2219477/latest.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
- OA URL
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https://www.researchsquare.com/article/rs-2219477/latest.pdfDirect OA link when available
- Concepts
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Umbilical cord, Tissue engineering, Medicine, Human heart, Heart valve, Pathology, Cardiology, Internal medicine, Biomedical engineering, AnatomyTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2024: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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