Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin Article Swipe
Aline Meneguzzi
,
Enéas Ricardo Konzen
,
Márcio Carlos Navroski
,
Samila Silva Camargo
,
Mariane de Oliveira Pereira
,
Léo Rufato
,
Queli Cristina Lovatel
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.4336/2019.pfb.39e201701550
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.4336/2019.pfb.39e201701550
Sequoia sempervirens (D. Don) Endl. is a conifer which produces high-quality wood with potential industrial applications. However, the species shows low germination rates. This study was devoted to optimizing a protocol for shoot multiplication of S. sempervirens through micropropagation. Two genotypes, adapted to southern Brazil, constituted the source of explants for consecutive experiments aiming in vitro multiplication. We used the traditional MS (Murashige & Skoog) culture medium at 50% of its original concentration, supplemented with plant growth regulators under two approaches. For multiplication we combined α-naphtalene acetic acid (NAA) and 6-benzilaminopurine (BAP) at distinct concentrations. In the second experiment, we tested concentrations of three cytokinins types (BAP, kinetin and 2-isopentheiladenine ). NAA at 0.1 mg L-1 enabled the production of shoots with higher mean length for the main branch. The use of kinetin in low concentrations provided the best performance for shoot multiplication, differing between two genotypes. We outline a recommendation of the most suitable plant growth regulators and their concentration for shoot multiplication of S. sempervirens, which might assist further work aiming at adventitious rooting and acclimatization.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.4336/2019.pfb.39e201701550
- https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/download/1550/904
- OA Status
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- Cited By
- 2
- References
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- Related Works
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- OpenAlex ID
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- DOI
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https://doi.org/10.4336/2019.pfb.39e201701550Digital Object Identifier
- Title
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Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetinWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
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2019-12-23Full publication date if available
- Authors
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Aline Meneguzzi, Enéas Ricardo Konzen, Márcio Carlos Navroski, Samila Silva Camargo, Mariane de Oliveira Pereira, Léo Rufato, Queli Cristina LovatelList of authors in order
- Landing page
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https://doi.org/10.4336/2019.pfb.39e201701550Publisher landing page
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https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/download/1550/904Direct link to full text PDF
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YesWhether a free full text is available
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diamondOpen access status per OpenAlex
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https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/download/1550/904Direct OA link when available
- Concepts
-
Horticulture, Sequoia, Kinetin, Physics, Botany, Biology, Explant culture, In vitro, BiochemistryTop concepts (fields/topics) attached by OpenAlex
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2Total citation count in OpenAlex
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2023: 1, 2021: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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