Simultaneous detection of multiple spoilage bacteria using a fluorogenic loop-mediated isothermal amplification-based multi-sample microfluidic chip Article Swipe
Yirong Lou
,
Yuxin Chen
,
Qianjin Zhou
,
Jianmei Zhou
,
Zhong-Jie Xu
,
Wenjie Wang
,
Fangjie Chen
,
Hongxian Chen
,
Guizong Xu
,
Yanjun Zhang
,
Xizhi Shi
,
Jiong Chen
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1016/j.lwt.2024.115758
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1016/j.lwt.2024.115758
Spoilage bacteria, which ubiquitously contribute to food spoilage, pose a global public health risk. In this study, we developed a centrifugal microfluidic chip integrating fluorogenic loop-mediated isothermal amplification (LAMP) to detect six important spoilage bacteria: Bacillus cereus, Clostridium botulinum, Photobacterium phosphoreum, Pseudomonas putida, Streptococcus agalactiae, and Shewanella putrefaciens. The LAMP reaction components were pre-mixed (primers pre-embedded in each reaction chamber of the chip) and evenly pipetted into the two inlet holes, enabling the simultaneous analysis of six genetic targets from a single sample. This facilitates the automated detection of up to four samples simultaneously using a single chip. The LAMP reactions were conducted in a 5-μL volume at 65 °C for 60 min, achieving limits of detection ranging from 10−1 to 10−2 pg/μL of bacterial genomic DNA. Almost all coefficients of variation for time-to-positive values consistently remained under 10% when genomic DNA was used at concentrations within the limits of detection for the six bacteria. This demonstrated excellent reproducibility across replicates. The clinical sensitivity and specificity for field sample detection were 93.3% and 97.5%, respectively. This method offers ease of operation, rapid analysis, and minimal reagent usage and has great potential for the on-site detection of multiple spoilage bacteria.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.lwt.2024.115758
- OA Status
- gold
- Cited By
- 3
- References
- 57
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4391095926
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4391095926Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.lwt.2024.115758Digital Object Identifier
- Title
-
Simultaneous detection of multiple spoilage bacteria using a fluorogenic loop-mediated isothermal amplification-based multi-sample microfluidic chipWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2024Year of publication
- Publication date
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2024-01-22Full publication date if available
- Authors
-
Yirong Lou, Yuxin Chen, Qianjin Zhou, Jianmei Zhou, Zhong-Jie Xu, Wenjie Wang, Fangjie Chen, Hongxian Chen, Guizong Xu, Yanjun Zhang, Xizhi Shi, Jiong ChenList of authors in order
- Landing page
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https://doi.org/10.1016/j.lwt.2024.115758Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1016/j.lwt.2024.115758Direct OA link when available
- Concepts
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Loop-mediated isothermal amplification, Microfluidic chip, Microfluidics, Chip, Food spoilage, Bacteria, Chromatography, Lab-on-a-chip, Sample (material), Chemistry, Nanotechnology, Materials science, Biology, Computer science, Biochemistry, DNA, Genetics, TelecommunicationsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
3Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3Per-year citation counts (last 5 years)
- References (count)
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57Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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