Single-cell analysis of Rohon-Beard neurons implicates Fgf signaling in axon maintenance and cell survival Article Swipe
Adam M. Tuttle
,
Lauren N. Miller
,
Lindsey J. Royer
,
Hua Wen
,
Jimmy J. Kelly
,
Nicholas L. Calistri
,
Laura M. Heiser
,
Alex Nechiporuk
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1101/2023.08.26.554953
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1101/2023.08.26.554953
Peripheral sensory neurons are a critical part of the nervous system that transmit a multitude of sensory stimuli to the central nervous system. During larval and juvenile stages in zebrafish, this function is mediated by Rohon-Beard somatosensory neurons (RBs). RBs are optically accessible and amenable to experimental manipulation, making them a powerful system for mechanistic investigation of sensory neurons. Previous studies provided evidence that RBs fall into multiple subclasses; however, the number and molecular make up of these potential RB subtypes have not been well defined. Using a single-cell RNA sequencing (scRNA-seq) approach, we demonstrate that larval RBs in zebrafish fall into three, largely non-overlapping classes of neurons. We also show that RBs are molecularly distinct from trigeminal neurons in zebrafish. Cross-species transcriptional analysis indicates that one RB subclass is similar to a mammalian group of A-fiber sensory neurons. Another RB subclass is predicted to sense multiple modalities, including mechanical stimulation and chemical irritants. We leveraged our scRNA-seq data to determine that the fibroblast growth factor (Fgf) pathway is active in RBs. Pharmacological and genetic inhibition of this pathway led to defects in axon maintenance and RB cell death. Moreover, this can be phenocopied by treatment with dovitinib, an FDA-approved Fgf inhibitor with a common side effect of peripheral neuropathy. Importantly, dovitinib-mediated axon loss can be suppressed by loss of Sarm1, a positive regulator of neuronal cell death and axonal injury. This offers a molecular target for future clinical intervention to fight neurotoxic effects of this drug.
Related Topics
Concepts
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2023.08.26.554953
- https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdf
- OA Status
- green
- Cited By
- 4
- References
- 75
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4386208570
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4386208570Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2023.08.26.554953Digital Object Identifier
- Title
-
Single-cell analysis of Rohon-Beard neurons implicates Fgf signaling in axon maintenance and cell survivalWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-08-28Full publication date if available
- Authors
-
Adam M. Tuttle, Lauren N. Miller, Lindsey J. Royer, Hua Wen, Jimmy J. Kelly, Nicholas L. Calistri, Laura M. Heiser, Alex NechiporukList of authors in order
- Landing page
-
https://doi.org/10.1101/2023.08.26.554953Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdfDirect OA link when available
- Concepts
-
Axon, Biology, Zebrafish, Fibroblast growth factor, Neuroscience, Nervous system, Sensory system, Cell biology, Peripheral nervous system, Central nervous system, Genetics, Gene, ReceptorTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
4Total citation count in OpenAlex
- Citations by year (recent)
-
2024: 3, 2023: 1Per-year citation counts (last 5 years)
- References (count)
-
75Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4386208570 |
|---|---|
| doi | https://doi.org/10.1101/2023.08.26.554953 |
| ids.doi | https://doi.org/10.1101/2023.08.26.554953 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/37693470 |
| ids.openalex | https://openalex.org/W4386208570 |
| fwci | 0.99049492 |
| type | preprint |
| title | Single-cell analysis of Rohon-Beard neurons implicates Fgf signaling in axon maintenance and cell survival |
| biblio.issue | |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T11685 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9991000294685364 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1307 |
| topics[0].subfield.display_name | Cell Biology |
| topics[0].display_name | Zebrafish Biomedical Research Applications |
| topics[1].id | https://openalex.org/T11266 |
| topics[1].field.id | https://openalex.org/fields/28 |
| topics[1].field.display_name | Neuroscience |
| topics[1].score | 0.9959999918937683 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2808 |
| topics[1].subfield.display_name | Neurology |
| topics[1].display_name | Neuroinflammation and Neurodegeneration Mechanisms |
| topics[2].id | https://openalex.org/T11178 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9929999709129333 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1312 |
| topics[2].subfield.display_name | Molecular Biology |
| topics[2].display_name | Receptor Mechanisms and Signaling |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C2779530196 |
| concepts[0].level | 2 |
| concepts[0].score | 0.707781195640564 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q178999 |
| concepts[0].display_name | Axon |
| concepts[1].id | https://openalex.org/C86803240 |
| concepts[1].level | 0 |
| concepts[1].score | 0.6969383955001831 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[1].display_name | Biology |
| concepts[2].id | https://openalex.org/C2776878037 |
| concepts[2].level | 3 |
| concepts[2].score | 0.6963841319084167 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q169444 |
| concepts[2].display_name | Zebrafish |
| concepts[3].id | https://openalex.org/C74373430 |
| concepts[3].level | 3 |
| concepts[3].score | 0.6479572653770447 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q24727126 |
| concepts[3].display_name | Fibroblast growth factor |
| concepts[4].id | https://openalex.org/C169760540 |
| concepts[4].level | 1 |
| concepts[4].score | 0.6240534782409668 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q207011 |
| concepts[4].display_name | Neuroscience |
| concepts[5].id | https://openalex.org/C545706735 |
| concepts[5].level | 2 |
| concepts[5].score | 0.572797417640686 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q9404 |
| concepts[5].display_name | Nervous system |
| concepts[6].id | https://openalex.org/C94487597 |
| concepts[6].level | 2 |
| concepts[6].score | 0.49510422348976135 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q11101 |
| concepts[6].display_name | Sensory system |
| concepts[7].id | https://openalex.org/C95444343 |
| concepts[7].level | 1 |
| concepts[7].score | 0.45159268379211426 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q7141 |
| concepts[7].display_name | Cell biology |
| concepts[8].id | https://openalex.org/C545314008 |
| concepts[8].level | 3 |
| concepts[8].score | 0.4428202509880066 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q169953 |
| concepts[8].display_name | Peripheral nervous system |
| concepts[9].id | https://openalex.org/C529278444 |
| concepts[9].level | 2 |
| concepts[9].score | 0.31008607149124146 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q47273 |
| concepts[9].display_name | Central nervous system |
| concepts[10].id | https://openalex.org/C54355233 |
| concepts[10].level | 1 |
| concepts[10].score | 0.1909378468990326 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[10].display_name | Genetics |
| concepts[11].id | https://openalex.org/C104317684 |
| concepts[11].level | 2 |
| concepts[11].score | 0.0 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[11].display_name | Gene |
| concepts[12].id | https://openalex.org/C170493617 |
| concepts[12].level | 2 |
| concepts[12].score | 0.0 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q208467 |
| concepts[12].display_name | Receptor |
| keywords[0].id | https://openalex.org/keywords/axon |
| keywords[0].score | 0.707781195640564 |
| keywords[0].display_name | Axon |
| keywords[1].id | https://openalex.org/keywords/biology |
| keywords[1].score | 0.6969383955001831 |
| keywords[1].display_name | Biology |
| keywords[2].id | https://openalex.org/keywords/zebrafish |
| keywords[2].score | 0.6963841319084167 |
| keywords[2].display_name | Zebrafish |
| keywords[3].id | https://openalex.org/keywords/fibroblast-growth-factor |
| keywords[3].score | 0.6479572653770447 |
| keywords[3].display_name | Fibroblast growth factor |
| keywords[4].id | https://openalex.org/keywords/neuroscience |
| keywords[4].score | 0.6240534782409668 |
| keywords[4].display_name | Neuroscience |
| keywords[5].id | https://openalex.org/keywords/nervous-system |
| keywords[5].score | 0.572797417640686 |
| keywords[5].display_name | Nervous system |
| keywords[6].id | https://openalex.org/keywords/sensory-system |
| keywords[6].score | 0.49510422348976135 |
| keywords[6].display_name | Sensory system |
| keywords[7].id | https://openalex.org/keywords/cell-biology |
| keywords[7].score | 0.45159268379211426 |
| keywords[7].display_name | Cell biology |
| keywords[8].id | https://openalex.org/keywords/peripheral-nervous-system |
| keywords[8].score | 0.4428202509880066 |
| keywords[8].display_name | Peripheral nervous system |
| keywords[9].id | https://openalex.org/keywords/central-nervous-system |
| keywords[9].score | 0.31008607149124146 |
| keywords[9].display_name | Central nervous system |
| keywords[10].id | https://openalex.org/keywords/genetics |
| keywords[10].score | 0.1909378468990326 |
| keywords[10].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.1101/2023.08.26.554953 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4306402567 |
| locations[0].source.issn | |
| locations[0].source.type | repository |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| locations[0].source.host_organization | https://openalex.org/I2750212522 |
| locations[0].source.host_organization_name | Cold Spring Harbor Laboratory |
| locations[0].source.host_organization_lineage | https://openalex.org/I2750212522 |
| locations[0].license | |
| locations[0].pdf_url | https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdf |
| locations[0].version | acceptedVersion |
| locations[0].raw_type | posted-content |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | False |
| locations[0].raw_source_name | |
| locations[0].landing_page_url | https://doi.org/10.1101/2023.08.26.554953 |
| locations[1].id | pmid:37693470 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | bioRxiv : the preprint server for biology |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/37693470 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:10491107 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | bioRxiv |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/10491107 |
| indexed_in | crossref, pubmed |
| authorships[0].author.id | https://openalex.org/A5008779420 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-4300-3848 |
| authorships[0].author.display_name | Adam M. Tuttle |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[0].affiliations[0].raw_affiliation_string | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[0].institutions[0].id | https://openalex.org/I165690674 |
| authorships[0].institutions[0].ror | https://ror.org/009avj582 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | Oregon Health & Science University |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Adam M. Tuttle |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[1].author.id | https://openalex.org/A5102727449 |
| authorships[1].author.orcid | https://orcid.org/0000-0001-6982-5041 |
| authorships[1].author.display_name | Lauren N. Miller |
| authorships[1].countries | US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[1].affiliations[0].raw_affiliation_string | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[1].institutions[0].id | https://openalex.org/I165690674 |
| authorships[1].institutions[0].ror | https://ror.org/009avj582 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[1].institutions[0].country_code | US |
| authorships[1].institutions[0].display_name | Oregon Health & Science University |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Lauren N. Miller |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[2].author.id | https://openalex.org/A5112988566 |
| authorships[2].author.orcid | |
| authorships[2].author.display_name | Lindsey J. Royer |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[2].affiliations[0].raw_affiliation_string | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[2].institutions[0].id | https://openalex.org/I165690674 |
| authorships[2].institutions[0].ror | https://ror.org/009avj582 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Oregon Health & Science University |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Lindsey J. Royer |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[3].author.id | https://openalex.org/A5101428657 |
| authorships[3].author.orcid | https://orcid.org/0009-0009-5326-8741 |
| authorships[3].author.display_name | Hua Wen |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I165690674, https://openalex.org/I2802707198 |
| authorships[3].affiliations[0].raw_affiliation_string | Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[3].institutions[0].id | https://openalex.org/I165690674 |
| authorships[3].institutions[0].ror | https://ror.org/009avj582 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | Oregon Health & Science University |
| authorships[3].institutions[1].id | https://openalex.org/I2802707198 |
| authorships[3].institutions[1].ror | https://ror.org/054hm6j14 |
| authorships[3].institutions[1].type | facility |
| authorships[3].institutions[1].lineage | https://openalex.org/I2802707198 |
| authorships[3].institutions[1].country_code | US |
| authorships[3].institutions[1].display_name | Vollum Institute |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Hua Wen |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[4].author.id | https://openalex.org/A5004112895 |
| authorships[4].author.orcid | https://orcid.org/0009-0007-5807-0676 |
| authorships[4].author.display_name | Jimmy J. Kelly |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I165690674, https://openalex.org/I2802707198 |
| authorships[4].affiliations[0].raw_affiliation_string | Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[4].institutions[0].id | https://openalex.org/I165690674 |
| authorships[4].institutions[0].ror | https://ror.org/009avj582 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | Oregon Health & Science University |
| authorships[4].institutions[1].id | https://openalex.org/I2802707198 |
| authorships[4].institutions[1].ror | https://ror.org/054hm6j14 |
| authorships[4].institutions[1].type | facility |
| authorships[4].institutions[1].lineage | https://openalex.org/I2802707198 |
| authorships[4].institutions[1].country_code | US |
| authorships[4].institutions[1].display_name | Vollum Institute |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Jimmy J. Kelly |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[5].author.id | https://openalex.org/A5033042293 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-8541-0919 |
| authorships[5].author.display_name | Nicholas L. Calistri |
| authorships[5].countries | US |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[5].affiliations[0].raw_affiliation_string | Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[5].institutions[0].id | https://openalex.org/I165690674 |
| authorships[5].institutions[0].ror | https://ror.org/009avj582 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[5].institutions[0].country_code | US |
| authorships[5].institutions[0].display_name | Oregon Health & Science University |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Nicholas L. Calistri |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[6].author.id | https://openalex.org/A5084824340 |
| authorships[6].author.orcid | https://orcid.org/0000-0003-3330-0950 |
| authorships[6].author.display_name | Laura M. Heiser |
| authorships[6].countries | US |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[6].affiliations[0].raw_affiliation_string | Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[6].institutions[0].id | https://openalex.org/I165690674 |
| authorships[6].institutions[0].ror | https://ror.org/009avj582 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[6].institutions[0].country_code | US |
| authorships[6].institutions[0].display_name | Oregon Health & Science University |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Laura M. Heiser |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[7].author.id | https://openalex.org/A5033428495 |
| authorships[7].author.orcid | https://orcid.org/0000-0002-8295-8188 |
| authorships[7].author.display_name | Alex Nechiporuk |
| authorships[7].countries | US |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I165690674 |
| authorships[7].affiliations[0].raw_affiliation_string | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| authorships[7].institutions[0].id | https://openalex.org/I165690674 |
| authorships[7].institutions[0].ror | https://ror.org/009avj582 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I165690674 |
| authorships[7].institutions[0].country_code | US |
| authorships[7].institutions[0].display_name | Oregon Health & Science University |
| authorships[7].author_position | last |
| authorships[7].raw_author_name | Alex V. Nechiporuk |
| authorships[7].is_corresponding | True |
| authorships[7].raw_affiliation_strings | Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, Oregon, USA |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdf |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Single-cell analysis of Rohon-Beard neurons implicates Fgf signaling in axon maintenance and cell survival |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11685 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9991000294685364 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1307 |
| primary_topic.subfield.display_name | Cell Biology |
| primary_topic.display_name | Zebrafish Biomedical Research Applications |
| related_works | https://openalex.org/W1980605506, https://openalex.org/W1594292955, https://openalex.org/W3183365292, https://openalex.org/W2808283345, https://openalex.org/W4387669168, https://openalex.org/W4236834903, https://openalex.org/W141104486, https://openalex.org/W2495108552, https://openalex.org/W191420785, https://openalex.org/W2142738321 |
| cited_by_count | 4 |
| counts_by_year[0].year | 2024 |
| counts_by_year[0].cited_by_count | 3 |
| counts_by_year[1].year | 2023 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1101/2023.08.26.554953 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4306402567 |
| best_oa_location.source.issn | |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| best_oa_location.source.host_organization | https://openalex.org/I2750212522 |
| best_oa_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| best_oa_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| best_oa_location.license | |
| best_oa_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdf |
| best_oa_location.version | acceptedVersion |
| best_oa_location.raw_type | posted-content |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | |
| best_oa_location.landing_page_url | https://doi.org/10.1101/2023.08.26.554953 |
| primary_location.id | doi:10.1101/2023.08.26.554953 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4306402567 |
| primary_location.source.issn | |
| primary_location.source.type | repository |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| primary_location.source.host_organization | https://openalex.org/I2750212522 |
| primary_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| primary_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| primary_location.license | |
| primary_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2023/08/31/2023.08.26.554953.full.pdf |
| primary_location.version | acceptedVersion |
| primary_location.raw_type | posted-content |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | False |
| primary_location.raw_source_name | |
| primary_location.landing_page_url | https://doi.org/10.1101/2023.08.26.554953 |
| publication_date | 2023-08-28 |
| publication_year | 2023 |
| referenced_works | https://openalex.org/W4285492200, https://openalex.org/W1989837824, https://openalex.org/W2103017472, https://openalex.org/W2009967312, https://openalex.org/W4381550198, https://openalex.org/W3163504032, https://openalex.org/W1981358093, https://openalex.org/W2005106433, https://openalex.org/W4285798898, https://openalex.org/W2125779893, https://openalex.org/W2168633862, https://openalex.org/W1969780631, https://openalex.org/W3121063139, https://openalex.org/W1980429214, https://openalex.org/W2791294509, https://openalex.org/W2989923168, https://openalex.org/W2750650202, https://openalex.org/W2030047746, https://openalex.org/W2537687146, https://openalex.org/W2062200458, https://openalex.org/W3124549379, https://openalex.org/W3024416752, https://openalex.org/W2160806427, https://openalex.org/W2035257142, https://openalex.org/W4317746825, https://openalex.org/W2152251876, https://openalex.org/W4385239985, https://openalex.org/W2125349610, https://openalex.org/W3154885935, https://openalex.org/W2942435013, https://openalex.org/W3081268457, https://openalex.org/W2088130353, https://openalex.org/W3195719526, https://openalex.org/W2304751114, https://openalex.org/W2105288369, https://openalex.org/W2006171398, https://openalex.org/W2008776459, https://openalex.org/W2899622246, https://openalex.org/W3185642961, https://openalex.org/W2387227493, https://openalex.org/W2162585555, https://openalex.org/W2127613537, https://openalex.org/W2057887205, https://openalex.org/W2086185798, https://openalex.org/W2068380982, https://openalex.org/W2034075580, https://openalex.org/W2114546997, https://openalex.org/W2037769556, https://openalex.org/W2048261081, https://openalex.org/W2093336699, https://openalex.org/W2026016034, https://openalex.org/W2134881801, https://openalex.org/W1969712770, https://openalex.org/W2011376300, https://openalex.org/W2884820450, https://openalex.org/W2059574293, https://openalex.org/W1997136553, https://openalex.org/W2058825331, https://openalex.org/W2948880437, https://openalex.org/W2167279371, https://openalex.org/W2786883050, https://openalex.org/W3196682570, https://openalex.org/W1986826174, https://openalex.org/W2120229762, https://openalex.org/W3003400347, https://openalex.org/W4288183200, https://openalex.org/W2102109465, https://openalex.org/W2578197673, https://openalex.org/W1995704024, https://openalex.org/W1982275211, https://openalex.org/W2087004322, https://openalex.org/W2081615974, https://openalex.org/W2129515255, https://openalex.org/W2583133006, https://openalex.org/W4220688905 |
| referenced_works_count | 75 |
| abstract_inverted_index.a | 5, 14, 51, 88, 133, 204, 222, 234 |
| abstract_inverted_index.RB | 80, 128, 141, 187 |
| abstract_inverted_index.We | 109, 155 |
| abstract_inverted_index.an | 199 |
| abstract_inverted_index.be | 193, 216 |
| abstract_inverted_index.by | 35, 195, 218 |
| abstract_inverted_index.in | 29, 99, 120, 171, 183 |
| abstract_inverted_index.is | 33, 130, 143, 169 |
| abstract_inverted_index.of | 8, 16, 57, 77, 107, 136, 177, 208, 220, 225, 245 |
| abstract_inverted_index.to | 19, 46, 132, 145, 160, 181, 241 |
| abstract_inverted_index.up | 76 |
| abstract_inverted_index.we | 94 |
| abstract_inverted_index.Fgf | 201 |
| abstract_inverted_index.RBs | 40, 65, 98, 113 |
| abstract_inverted_index.RNA | 90 |
| abstract_inverted_index.and | 26, 44, 73, 152, 174, 186, 229 |
| abstract_inverted_index.are | 4, 41, 114 |
| abstract_inverted_index.can | 192, 215 |
| abstract_inverted_index.for | 54, 237 |
| abstract_inverted_index.led | 180 |
| abstract_inverted_index.not | 83 |
| abstract_inverted_index.one | 127 |
| abstract_inverted_index.our | 157 |
| abstract_inverted_index.the | 9, 20, 71, 163 |
| abstract_inverted_index.RBs. | 172 |
| abstract_inverted_index.This | 232 |
| abstract_inverted_index.also | 110 |
| abstract_inverted_index.axon | 184, 213 |
| abstract_inverted_index.been | 84 |
| abstract_inverted_index.cell | 188, 227 |
| abstract_inverted_index.data | 159 |
| abstract_inverted_index.fall | 66, 101 |
| abstract_inverted_index.from | 117 |
| abstract_inverted_index.have | 82 |
| abstract_inverted_index.into | 67, 102 |
| abstract_inverted_index.loss | 214, 219 |
| abstract_inverted_index.make | 75 |
| abstract_inverted_index.part | 7 |
| abstract_inverted_index.show | 111 |
| abstract_inverted_index.side | 206 |
| abstract_inverted_index.that | 12, 64, 96, 112, 126, 162 |
| abstract_inverted_index.them | 50 |
| abstract_inverted_index.this | 31, 178, 191, 246 |
| abstract_inverted_index.well | 85 |
| abstract_inverted_index.with | 197, 203 |
| abstract_inverted_index.(Fgf) | 167 |
| abstract_inverted_index.Using | 87 |
| abstract_inverted_index.death | 228 |
| abstract_inverted_index.drug. | 247 |
| abstract_inverted_index.fight | 242 |
| abstract_inverted_index.group | 135 |
| abstract_inverted_index.sense | 146 |
| abstract_inverted_index.these | 78 |
| abstract_inverted_index.(RBs). | 39 |
| abstract_inverted_index.During | 24 |
| abstract_inverted_index.Sarm1, | 221 |
| abstract_inverted_index.active | 170 |
| abstract_inverted_index.axonal | 230 |
| abstract_inverted_index.common | 205 |
| abstract_inverted_index.death. | 189 |
| abstract_inverted_index.effect | 207 |
| abstract_inverted_index.factor | 166 |
| abstract_inverted_index.future | 238 |
| abstract_inverted_index.growth | 165 |
| abstract_inverted_index.larval | 25, 97 |
| abstract_inverted_index.making | 49 |
| abstract_inverted_index.number | 72 |
| abstract_inverted_index.offers | 233 |
| abstract_inverted_index.stages | 28 |
| abstract_inverted_index.system | 11, 53 |
| abstract_inverted_index.target | 236 |
| abstract_inverted_index.three, | 103 |
| abstract_inverted_index.A-fiber | 137 |
| abstract_inverted_index.Another | 140 |
| abstract_inverted_index.central | 21 |
| abstract_inverted_index.classes | 106 |
| abstract_inverted_index.defects | 182 |
| abstract_inverted_index.effects | 244 |
| abstract_inverted_index.genetic | 175 |
| abstract_inverted_index.injury. | 231 |
| abstract_inverted_index.largely | 104 |
| abstract_inverted_index.nervous | 10, 22 |
| abstract_inverted_index.neurons | 3, 38, 119 |
| abstract_inverted_index.pathway | 168, 179 |
| abstract_inverted_index.sensory | 2, 17, 58, 138 |
| abstract_inverted_index.similar | 131 |
| abstract_inverted_index.stimuli | 18 |
| abstract_inverted_index.studies | 61 |
| abstract_inverted_index.system. | 23 |
| abstract_inverted_index.ABSTRACT | 0 |
| abstract_inverted_index.Previous | 60 |
| abstract_inverted_index.amenable | 45 |
| abstract_inverted_index.analysis | 124 |
| abstract_inverted_index.chemical | 153 |
| abstract_inverted_index.clinical | 239 |
| abstract_inverted_index.critical | 6 |
| abstract_inverted_index.defined. | 86 |
| abstract_inverted_index.distinct | 116 |
| abstract_inverted_index.evidence | 63 |
| abstract_inverted_index.function | 32 |
| abstract_inverted_index.however, | 70 |
| abstract_inverted_index.juvenile | 27 |
| abstract_inverted_index.mediated | 34 |
| abstract_inverted_index.multiple | 68, 147 |
| abstract_inverted_index.neuronal | 226 |
| abstract_inverted_index.neurons. | 59, 108, 139 |
| abstract_inverted_index.positive | 223 |
| abstract_inverted_index.powerful | 52 |
| abstract_inverted_index.provided | 62 |
| abstract_inverted_index.subclass | 129, 142 |
| abstract_inverted_index.subtypes | 81 |
| abstract_inverted_index.transmit | 13 |
| abstract_inverted_index.Moreover, | 190 |
| abstract_inverted_index.approach, | 93 |
| abstract_inverted_index.determine | 161 |
| abstract_inverted_index.including | 149 |
| abstract_inverted_index.indicates | 125 |
| abstract_inverted_index.inhibitor | 202 |
| abstract_inverted_index.leveraged | 156 |
| abstract_inverted_index.mammalian | 134 |
| abstract_inverted_index.molecular | 74, 235 |
| abstract_inverted_index.multitude | 15 |
| abstract_inverted_index.optically | 42 |
| abstract_inverted_index.potential | 79 |
| abstract_inverted_index.predicted | 144 |
| abstract_inverted_index.regulator | 224 |
| abstract_inverted_index.scRNA-seq | 158 |
| abstract_inverted_index.treatment | 196 |
| abstract_inverted_index.zebrafish | 100 |
| abstract_inverted_index.Peripheral | 1 |
| abstract_inverted_index.accessible | 43 |
| abstract_inverted_index.dovitinib, | 198 |
| abstract_inverted_index.fibroblast | 164 |
| abstract_inverted_index.inhibition | 176 |
| abstract_inverted_index.irritants. | 154 |
| abstract_inverted_index.mechanical | 150 |
| abstract_inverted_index.neurotoxic | 243 |
| abstract_inverted_index.peripheral | 209 |
| abstract_inverted_index.sequencing | 91 |
| abstract_inverted_index.suppressed | 217 |
| abstract_inverted_index.trigeminal | 118 |
| abstract_inverted_index.zebrafish, | 30 |
| abstract_inverted_index.zebrafish. | 121 |
| abstract_inverted_index.(scRNA-seq) | 92 |
| abstract_inverted_index.Rohon-Beard | 36 |
| abstract_inverted_index.demonstrate | 95 |
| abstract_inverted_index.maintenance | 185 |
| abstract_inverted_index.mechanistic | 55 |
| abstract_inverted_index.modalities, | 148 |
| abstract_inverted_index.molecularly | 115 |
| abstract_inverted_index.neuropathy. | 210 |
| abstract_inverted_index.phenocopied | 194 |
| abstract_inverted_index.single-cell | 89 |
| abstract_inverted_index.stimulation | 151 |
| abstract_inverted_index.subclasses; | 69 |
| abstract_inverted_index.FDA-approved | 200 |
| abstract_inverted_index.Importantly, | 211 |
| abstract_inverted_index.experimental | 47 |
| abstract_inverted_index.intervention | 240 |
| abstract_inverted_index.Cross-species | 122 |
| abstract_inverted_index.investigation | 56 |
| abstract_inverted_index.manipulation, | 48 |
| abstract_inverted_index.somatosensory | 37 |
| abstract_inverted_index.Pharmacological | 173 |
| abstract_inverted_index.non-overlapping | 105 |
| abstract_inverted_index.transcriptional | 123 |
| abstract_inverted_index.dovitinib-mediated | 212 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 89 |
| corresponding_author_ids | https://openalex.org/A5033428495 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 8 |
| corresponding_institution_ids | https://openalex.org/I165690674 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/3 |
| sustainable_development_goals[0].score | 0.44999998807907104 |
| sustainable_development_goals[0].display_name | Good health and well-being |
| citation_normalized_percentile.value | 0.75947704 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |