Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans Article Swipe
Fredrik Noborn
,
Alejandro Gómez Toledo
,
Anders Green
,
Waqas Nasir
,
Carina Sihlbom
,
Jonas Nilsson
,
Göran Larson
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1038/srep34537
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1038/srep34537
Heparan sulfate (HS) and chondroitin sulfate (CS) are complex polysaccharides that regulate important biological pathways in virtually all metazoan organisms. The polysaccharides often display opposite effects on cell functions with HS and CS structural motifs presenting unique binding sites for specific ligands. Still, the mechanisms by which glycan biosynthesis generates complex HS and CS polysaccharides required for the regulation of mammalian physiology remain elusive. Here we present a glycoproteomic approach that identifies and differentiates between HS and CS attachment sites and provides identity to the core proteins. Glycopeptides were prepared from perlecan, a complex proteoglycan known to be substituted with both HS and CS chains, further digested with heparinase or chondroitinase ABC to reduce the HS and CS chain lengths respectively and thereafter analyzed by nLC-MS/MS. This protocol enabled the identification of three consensus HS sites and one hybrid site, carrying either a HS or a CS chain. Inspection of the amino acid sequence at the hybrid attachment locus indicates that certain peptide motifs may encode for the chain type selection process. This analytical approach will become useful when addressing fundamental questions in basic biology specifically in elucidating the functional roles of site-specific glycosylations of proteoglycans.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/srep34537
- https://www.nature.com/articles/srep34537.pdf
- OA Status
- gold
- Cited By
- 40
- References
- 47
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2528850038
All OpenAlex metadata
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- OpenAlex ID
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https://openalex.org/W2528850038Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1038/srep34537Digital Object Identifier
- Title
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Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycansWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2016Year of publication
- Publication date
-
2016-10-03Full publication date if available
- Authors
-
Fredrik Noborn, Alejandro Gómez Toledo, Anders Green, Waqas Nasir, Carina Sihlbom, Jonas Nilsson, Göran LarsonList of authors in order
- Landing page
-
https://doi.org/10.1038/srep34537Publisher landing page
- PDF URL
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https://www.nature.com/articles/srep34537.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.nature.com/articles/srep34537.pdfDirect OA link when available
- Concepts
-
Glycan, Chondroitin sulfate, Heparan sulfate, Biochemistry, Proteoglycan, Perlecan, Peptide sequence, Chondroitin, Amino acid, Glycobiology, Structural motif, Glycosaminoglycan, Chemistry, Biology, Computational biology, Gene, Glycoprotein, Extracellular matrixTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
40Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3, 2024: 3, 2023: 1, 2022: 6, 2021: 7Per-year citation counts (last 5 years)
- References (count)
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47Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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