Small-Scale Fluidized Bed Bioreactor for Long-Term Dynamic Culture of 3D Cell Constructs and in vitro Testing Article Swipe
Joana Mendonça da Silva
,
Eloy Erro
,
Maooz Awan
,
Sherri‐Ann Chalmers
,
Barry Fuller
,
Clare Selden
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.3389/fbioe.2020.00895
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.3389/fbioe.2020.00895
With the increasing interest in three-dimensional (3D) cell constructs that better represent native tissues, comes the need to also invest in devices, i.e., bioreactors, that provide a controlled dynamic environment similar to the perfusion mechanism observed in vivo. Here a laboratory-scale fluidized bed bioreactor (sFBB) was designed for hydrogel (i.e., alginate) encapsulated cells to generate a dynamic culture system that produced a homogenous milieu and host substantial biomass for long-term evolution of tissue-like structures and "per cell" performance analysis. The bioreactor design, conceptualized through scale-down empirical similarity rules, was initially validated through computational fluid dynamics analysis for the distributor capacity of homogenously dispersing the flow with an average fluid velocity of 4.596 × 10-4 m/s. Experimental tests then demonstrated a consistent fluidization of hydrogel spheres, while maintaining shape and integrity (606.9 ± 99.3 μm diameter and 0.96 shape factor). It also induced mass transfer in and out of the hydrogel at a faster rate than static conditions. Finally, the sFBB sustained culture of alginate encapsulated hepatoblastoma cells for 12 days promoting proliferation into highly viable (>97%) cell spheroids at a high final density of 27.3 ± 0.78 million cells/mL beads. This was reproducible across multiple units set up in parallel and operating simultaneously. The sFBB prototype constitutes a simple and robust tool to generate 3D cell constructs, expandable into a multi-unit setup for simultaneous observations and for future development and biological evaluation of in vitro tissue models and their responses to different agents, increasing the complexity and speed of R&D processes.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fbioe.2020.00895
- https://www.frontiersin.org/articles/10.3389/fbioe.2020.00895/pdf
- OA Status
- gold
- Cited By
- 20
- References
- 77
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3081131802
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3081131802Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fbioe.2020.00895Digital Object Identifier
- Title
-
Small-Scale Fluidized Bed Bioreactor for Long-Term Dynamic Culture of 3D Cell Constructs and in vitro TestingWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-08-20Full publication date if available
- Authors
-
Joana Mendonça da Silva, Eloy Erro, Maooz Awan, Sherri‐Ann Chalmers, Barry Fuller, Clare SeldenList of authors in order
- Landing page
-
https://doi.org/10.3389/fbioe.2020.00895Publisher landing page
- PDF URL
-
https://www.frontiersin.org/articles/10.3389/fbioe.2020.00895/pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.frontiersin.org/articles/10.3389/fbioe.2020.00895/pdfDirect OA link when available
- Concepts
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Bioreactor, Biological system, Biomedical engineering, 3D cell culture, Scale (ratio), Computer science, Materials science, Process engineering, Chemistry, In vitro, Biology, Engineering, Physics, Organic chemistry, Quantum mechanics, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
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20Total citation count in OpenAlex
- Citations by year (recent)
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2025: 4, 2024: 5, 2023: 2, 2022: 7, 2021: 2Per-year citation counts (last 5 years)
- References (count)
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77Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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