sRNA_seq_clean_thrips_leafdiscs_timeseries Article Swipe
Marlot Westera
,
Petra Bleeker
·
YOU?
·
· 2039
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.8263052
YOU?
·
· 2039
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.8263052
2023-03 Timeseries experiment with clean thrips on leafdiscs of tomato MoneyMaker plants. srna and mrna were extracted with the kit 'NucleoSpin miRNA, Mini kit for miRNA and RNA purification'. contains (see metadata file for more info): - 20x leafdiscs samples as controls (5 biological replicates/wells for 4 timepoints: 3h, 6h, 9h, 12h) - 20x leafdiscs samples with thrips (5 biological replicates/wells for 4 timepoints: 3h, 6h, 9h, 12h) - 5x whole thrips feeding on leafdiscs for 12h (same experiment, as leafdiscs) - 2x the heads of thrips feeding on tomato for 24h+, i.e. salivary glands enriched samples - 2x the eggs of thrips Barcoded Small RNA-Seq libraries were generated from the small RNA according to the manufacturers’ protocols using the Small RNA-Seq Library Prep Kit (Lexogen). The size distribution of the libraries with indexed adapters was assessed using a 2200 TapeStation System with Agilent D1000 ScreenTapes (Agilent Technologies). The libraries were quantified on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) using the NEBNext Library Quant Kit for Illumina (New England BioLabs) according to the instructions of the manufacturer. The libraries were clustered and sequenced (75 bp) on a NextSeq 550 Sequencing System (Illumina) using a NextSeq 500/550 High Output Kit v2.5 (75 Cycles) (Illumina). Adapter: TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC
Related Topics
Metadata
- Type
- dataset
- Language
- en
- Landing Page
- https://doi.org/10.5281/zenodo.8263052
- OA Status
- green
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4393577347
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4393577347Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.5281/zenodo.8263052Digital Object Identifier
- Title
-
sRNA_seq_clean_thrips_leafdiscs_timeseriesWork title
- Type
-
datasetOpenAlex work type
- Language
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enPrimary language
- Publication year
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2039Year of publication
- Publication date
-
2039-04-01Full publication date if available
- Authors
-
Marlot Westera, Petra BleekerList of authors in order
- Landing page
-
https://doi.org/10.5281/zenodo.8263052Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.5281/zenodo.8263052Direct OA link when available
- Concepts
-
Thrips, Computer science, Computational biology, Biology, HorticultureTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- Related works (count)
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10Other works algorithmically related by OpenAlex
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