Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex Article Swipe
Joy Y. Wang
,
Christopher Hoel
,
Basem Al-Shayeb
,
Jillian F. Banfield
,
Stephen G. Brohawn
,
Jennifer A. Doudna
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1038/s41467-021-22900-y
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1038/s41467-021-22900-y
CRISPR-Cas systems provide adaptive immunity in bacteria and archaea, beginning with integration of foreign sequences into the host CRISPR genomic locus and followed by transcription and maturation of CRISPR RNAs (crRNAs). In some CRISPR systems, a reverse transcriptase (RT) fusion to the Cas1 integrase and Cas6 maturase creates a single protein that enables concerted sequence integration and crRNA production. To elucidate how the RT-integrase organizes distinct enzymatic activities, we present the cryo-EM structure of a Cas6-RT-Cas1—Cas2 CRISPR integrase complex. The structure reveals a heterohexamer in which the RT directly contacts the integrase and maturase domains, suggesting functional coordination between all three active sites. Together with biochemical experiments, our data support a model of sequential enzymatic activities that enable CRISPR sequence acquisition from RNA and DNA substrates. These findings highlight an expanded capacity of some CRISPR systems to acquire diverse sequences that direct CRISPR-mediated interference.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41467-021-22900-y
- https://www.nature.com/articles/s41467-021-22900-y.pdf
- OA Status
- gold
- Cited By
- 22
- References
- 81
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3157575462
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3157575462Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1038/s41467-021-22900-yDigital Object Identifier
- Title
-
Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complexWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-05-06Full publication date if available
- Authors
-
Joy Y. Wang, Christopher Hoel, Basem Al-Shayeb, Jillian F. Banfield, Stephen G. Brohawn, Jennifer A. DoudnaList of authors in order
- Landing page
-
https://doi.org/10.1038/s41467-021-22900-yPublisher landing page
- PDF URL
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https://www.nature.com/articles/s41467-021-22900-y.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
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https://www.nature.com/articles/s41467-021-22900-y.pdfDirect OA link when available
- Concepts
-
CRISPR, Integrase, Trans-activating crRNA, Computational biology, Biology, Reverse transcriptase, RNA, CRISPR interference, Genetics, DNA, Cas9, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
22Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3, 2024: 8, 2023: 3, 2022: 5, 2021: 3Per-year citation counts (last 5 years)
- References (count)
-
81Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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