Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for adventitious capture of cytoplasmic proteins Article Swipe
Xiaobing Zuo
,
Alexander Jussupow
,
Nina Ponomarenko
,
Nicholas M. Tefft
,
Neetu Singh Yadav
,
Kyleigh L. Range
,
Corie Y. Ralston
,
Michaela A. TerAvest
,
Markus Sutter
,
Cheryl A. Kerfeld
,
Josh V. Vermaas
,
Michael Feig
,
David M. Tiede
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.11.13.623460
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.11.13.623460
Bacterial microcompartments (BMCs) are self-assembling, protein shell structures that are widely investigated across a broad range of biological and abiotic chemistry applications. A central challenge in BMC research is the targeted capture of enzymes during shell assembly. While crystallography and cryo-EM techniques have been successful in determining BMC shell structures, there has been only limited success in visualizing the location of BMC-captured enzyme cargo. Here, we demonstrate the opportunity to use small angle X-ray scattering (SAXS) and pair density distribution function (PDDF) measurements combined with quantitative comparison to coordinate structure models as an approach to characterize BMC shell structures in solution conditions directly relevant to biochemical function. Using this approach, we analyzed BMC shells from Haliangium ochraceum that were isolated following expression in E. coli . The analysis allowed BMC shell structures and the extent of encapsulated enzyme cargo to be identified. Notably, the results demonstrate that HO-BMC shells adventitiously capture significant amounts of cytoplasmic cargo during assembly in E. coli . Our findings highlight the utility of SAXS/PDDF analysis for evaluating BMC architectures and enzyme encapsulation, offering valuable insights for designing BMC shells as platforms for biological and abiotic catalyst capture within confined environments.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2024.11.13.623460
- https://www.biorxiv.org/content/biorxiv/early/2024/11/15/2024.11.13.623460.full.pdf
- OA Status
- green
- References
- 40
- Related Works
- 10
- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W4404407125Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2024.11.13.623460Digital Object Identifier
- Title
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Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for adventitious capture of cytoplasmic proteinsWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-11-15Full publication date if available
- Authors
-
Xiaobing Zuo, Alexander Jussupow, Nina Ponomarenko, Nicholas M. Tefft, Neetu Singh Yadav, Kyleigh L. Range, Corie Y. Ralston, Michaela A. TerAvest, Markus Sutter, Cheryl A. Kerfeld, Josh V. Vermaas, Michael Feig, David M. TiedeList of authors in order
- Landing page
-
https://doi.org/10.1101/2024.11.13.623460Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2024/11/15/2024.11.13.623460.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2024/11/15/2024.11.13.623460.full.pdfDirect OA link when available
- Concepts
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Cytoplasm, Characterization (materials science), Scattering, Biophysics, Physics, Computational biology, Chemistry, Biology, Cell biology, OpticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- References (count)
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40Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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