Studies for lipase production ability of Aspergillus sp. strains from the Misiones rainforest of Paranaense. Article Swipe

PDF

Laura Ester Ortellado , Laura Lidia Villalba , Pedro Darío Zapata , María Isabel Fonseca ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.22541/au.169779096.64649062/v1

The lipases produced by fungi of the genus Aspergillus are mostly extracellular enzymes that are secreted into the environment. These lipases have broad substrate specificity and are capable of hydrolyzing a variety of lipids, including triglycerides, phospholipids, cholesterol esters, and free fatty acids, making them the focus of many studies on their applications. The present work carried out an exploratory analysis of the production of lipases in fungi of the genus Aspergillus isolated in the Misiones rainforest of Paranaense. Using qualitative detection techniques with Tween 80 % and rhodamine B as substrates, and based on the quantitative analysis conducted, it was determined that the isolate Aspergillus sp. LBM 054 exhibited the highest lipase production capacity with a total of 133 U mL-1. A Plackett-Burman statistical test found that adding tributyrin to the culture medium increased lipase activity (P<0.05). Among the evaluated concentrations, the addition of 2% tributyrin demonstrated the highest increase in lipase activity, reaching a fivefold increase compared to the initial activity observed at the beginning of the trials. The optimal activity and high stability at neutral to alkaline pH values make these enzymes suitable for various biotechnological applications. The zymogram gel of the selected strain showed an enzymatic profile, where the protein’s molecular mass was 38 kDa.

Related Topics

Concepts

Lipase Tributyrin Aspergillus Biochemistry Biology Enzyme Triacylglycerol lipase Food science Chemistry Chromatography Botany

Metadata

Type: preprint
Language: en
Landing Page: https://doi.org/10.22541/au.169779096.64649062/v1
PDF: https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062
OA Status: gold
Related Works: 10
OpenAlex ID: https://openalex.org/W4387816176

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4387816176

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.22541/au.169779096.64649062/v1

Digital Object Identifier
Title: Studies for lipase production ability of Aspergillus sp. strains from the Misiones rainforest of Paranaense.

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2023

Year of publication
Publication date: 2023-10-20

Full publication date if available
Authors: Laura Ester Ortellado, Laura Lidia Villalba, Pedro Darío Zapata, María Isabel Fonseca

List of authors in order
Landing page: https://doi.org/10.22541/au.169779096.64649062/v1

Publisher landing page
PDF URL: https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062

Direct link to full text PDF
Open access: Yes

Whether a free full text is available
OA status: gold

Open access status per OpenAlex
OA URL: https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062

Direct OA link when available
Concepts: Lipase, Tributyrin, Aspergillus, Biochemistry, Biology, Enzyme, Triacylglycerol lipase, Food science, Chemistry, Chromatography, Botany

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4387816176
doi	https://doi.org/10.22541/au.169779096.64649062/v1
ids.doi	https://doi.org/10.22541/au.169779096.64649062/v1
ids.openalex	https://openalex.org/W4387816176
fwci
type	preprint
title	Studies for lipase production ability of Aspergillus sp. strains from the Misiones rainforest of Paranaense.
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T14256
topics[0].field.id	https://openalex.org/fields/11
topics[0].field.display_name	Agricultural and Biological Sciences
topics[0].score	0.9693999886512756
topics[0].domain.id	https://openalex.org/domains/1
topics[0].domain.display_name	Life Sciences
topics[0].subfield.id	https://openalex.org/subfields/1107
topics[0].subfield.display_name	Forestry
topics[0].display_name	Agricultural and Food Sciences
topics[1].id	https://openalex.org/T10404
topics[1].field.id	https://openalex.org/fields/13
topics[1].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[1].score	0.9688000082969666
topics[1].domain.id	https://openalex.org/domains/1
topics[1].domain.display_name	Life Sciences
topics[1].subfield.id	https://openalex.org/subfields/1312
topics[1].subfield.display_name	Molecular Biology
topics[1].display_name	Enzyme Catalysis and Immobilization
topics[2].id	https://openalex.org/T12657
topics[2].field.id	https://openalex.org/fields/11
topics[2].field.display_name	Agricultural and Biological Sciences
topics[2].score	0.9222999811172485
topics[2].domain.id	https://openalex.org/domains/1
topics[2].domain.display_name	Life Sciences
topics[2].subfield.id	https://openalex.org/subfields/1110
topics[2].subfield.display_name	Plant Science
topics[2].display_name	Phytochemistry Medicinal Plant Applications
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C2779697368
concepts[0].level	3
concepts[0].score	0.7978066205978394
concepts[0].wikidata	https://www.wikidata.org/wiki/Q380967
concepts[0].display_name	Lipase
concepts[1].id	https://openalex.org/C2778855143
concepts[1].level	4
concepts[1].score	0.6335103511810303
concepts[1].wikidata	https://www.wikidata.org/wiki/Q4116129
concepts[1].display_name	Tributyrin
concepts[2].id	https://openalex.org/C2779175654
concepts[2].level	2
concepts[2].score	0.6175795197486877
concepts[2].wikidata	https://www.wikidata.org/wiki/Q335130
concepts[2].display_name	Aspergillus
concepts[3].id	https://openalex.org/C55493867
concepts[3].level	1
concepts[3].score	0.5171374678611755
concepts[3].wikidata	https://www.wikidata.org/wiki/Q7094
concepts[3].display_name	Biochemistry
concepts[4].id	https://openalex.org/C86803240
concepts[4].level	0
concepts[4].score	0.4692656397819519
concepts[4].wikidata	https://www.wikidata.org/wiki/Q420
concepts[4].display_name	Biology
concepts[5].id	https://openalex.org/C181199279
concepts[5].level	2
concepts[5].score	0.4662401080131531
concepts[5].wikidata	https://www.wikidata.org/wiki/Q8047
concepts[5].display_name	Enzyme
concepts[6].id	https://openalex.org/C2780786110
concepts[6].level	4
concepts[6].score	0.4226936101913452
concepts[6].wikidata	https://www.wikidata.org/wiki/Q7839871
concepts[6].display_name	Triacylglycerol lipase
concepts[7].id	https://openalex.org/C31903555
concepts[7].level	1
concepts[7].score	0.4141184091567993
concepts[7].wikidata	https://www.wikidata.org/wiki/Q1637030
concepts[7].display_name	Food science
concepts[8].id	https://openalex.org/C185592680
concepts[8].level	0
concepts[8].score	0.4075450003147125
concepts[8].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[8].display_name	Chemistry
concepts[9].id	https://openalex.org/C43617362
concepts[9].level	1
concepts[9].score	0.3585405945777893
concepts[9].wikidata	https://www.wikidata.org/wiki/Q170050
concepts[9].display_name	Chromatography
concepts[10].id	https://openalex.org/C59822182
concepts[10].level	1
concepts[10].score	0.20899710059165955
concepts[10].wikidata	https://www.wikidata.org/wiki/Q441
concepts[10].display_name	Botany
keywords[0].id	https://openalex.org/keywords/lipase
keywords[0].score	0.7978066205978394
keywords[0].display_name	Lipase
keywords[1].id	https://openalex.org/keywords/tributyrin
keywords[1].score	0.6335103511810303
keywords[1].display_name	Tributyrin
keywords[2].id	https://openalex.org/keywords/aspergillus
keywords[2].score	0.6175795197486877
keywords[2].display_name	Aspergillus
keywords[3].id	https://openalex.org/keywords/biochemistry
keywords[3].score	0.5171374678611755
keywords[3].display_name	Biochemistry
keywords[4].id	https://openalex.org/keywords/biology
keywords[4].score	0.4692656397819519
keywords[4].display_name	Biology
keywords[5].id	https://openalex.org/keywords/enzyme
keywords[5].score	0.4662401080131531
keywords[5].display_name	Enzyme
keywords[6].id	https://openalex.org/keywords/triacylglycerol-lipase
keywords[6].score	0.4226936101913452
keywords[6].display_name	Triacylglycerol lipase
keywords[7].id	https://openalex.org/keywords/food-science
keywords[7].score	0.4141184091567993
keywords[7].display_name	Food science
keywords[8].id	https://openalex.org/keywords/chemistry
keywords[8].score	0.4075450003147125
keywords[8].display_name	Chemistry
keywords[9].id	https://openalex.org/keywords/chromatography
keywords[9].score	0.3585405945777893
keywords[9].display_name	Chromatography
keywords[10].id	https://openalex.org/keywords/botany
keywords[10].score	0.20899710059165955
keywords[10].display_name	Botany
language	en
locations[0].id	doi:10.22541/au.169779096.64649062/v1
locations[0].is_oa	True
locations[0].source
locations[0].license
locations[0].pdf_url	https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062
locations[0].version	acceptedVersion
locations[0].raw_type	posted-content
locations[0].license_id
locations[0].is_accepted	True
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.22541/au.169779096.64649062/v1
indexed_in	crossref
authorships[0].author.id	https://openalex.org/A5059501018
authorships[0].author.orcid	https://orcid.org/0000-0002-7983-3288
authorships[0].author.display_name	Laura Ester Ortellado
authorships[0].countries	AR
authorships[0].affiliations[0].institution_ids	https://openalex.org/I17994240
authorships[0].affiliations[0].raw_affiliation_string	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[0].institutions[0].id	https://openalex.org/I17994240
authorships[0].institutions[0].ror	https://ror.org/03kr8x191
authorships[0].institutions[0].type	education
authorships[0].institutions[0].lineage	https://openalex.org/I17994240
authorships[0].institutions[0].country_code	AR
authorships[0].institutions[0].display_name	National University of Misiones
authorships[0].author_position	first
authorships[0].raw_author_name	Laura Ester Ortellado
authorships[0].is_corresponding	False
authorships[0].raw_affiliation_strings	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[1].author.id	https://openalex.org/A5027923644
authorships[1].author.orcid	https://orcid.org/0000-0002-7488-4238
authorships[1].author.display_name	Laura Lidia Villalba
authorships[1].countries	AR
authorships[1].affiliations[0].institution_ids	https://openalex.org/I17994240
authorships[1].affiliations[0].raw_affiliation_string	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[1].institutions[0].id	https://openalex.org/I17994240
authorships[1].institutions[0].ror	https://ror.org/03kr8x191
authorships[1].institutions[0].type	education
authorships[1].institutions[0].lineage	https://openalex.org/I17994240
authorships[1].institutions[0].country_code	AR
authorships[1].institutions[0].display_name	National University of Misiones
authorships[1].author_position	middle
authorships[1].raw_author_name	Laura Villalba
authorships[1].is_corresponding	False
authorships[1].raw_affiliation_strings	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[2].author.id	https://openalex.org/A5062186276
authorships[2].author.orcid	https://orcid.org/0000-0001-6476-8324
authorships[2].author.display_name	Pedro Darío Zapata
authorships[2].countries	AR
authorships[2].affiliations[0].institution_ids	https://openalex.org/I17994240
authorships[2].affiliations[0].raw_affiliation_string	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[2].institutions[0].id	https://openalex.org/I17994240
authorships[2].institutions[0].ror	https://ror.org/03kr8x191
authorships[2].institutions[0].type	education
authorships[2].institutions[0].lineage	https://openalex.org/I17994240
authorships[2].institutions[0].country_code	AR
authorships[2].institutions[0].display_name	National University of Misiones
authorships[2].author_position	middle
authorships[2].raw_author_name	Pedro Zapata
authorships[2].is_corresponding	False
authorships[2].raw_affiliation_strings	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[3].author.id	https://openalex.org/A5081571940
authorships[3].author.orcid	https://orcid.org/0000-0002-5150-9849
authorships[3].author.display_name	María Isabel Fonseca
authorships[3].countries	AR
authorships[3].affiliations[0].institution_ids	https://openalex.org/I17994240
authorships[3].affiliations[0].raw_affiliation_string	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
authorships[3].institutions[0].id	https://openalex.org/I17994240
authorships[3].institutions[0].ror	https://ror.org/03kr8x191
authorships[3].institutions[0].type	education
authorships[3].institutions[0].lineage	https://openalex.org/I17994240
authorships[3].institutions[0].country_code	AR
authorships[3].institutions[0].display_name	National University of Misiones
authorships[3].author_position	last
authorships[3].raw_author_name	Maria Fonseca
authorships[3].is_corresponding	False
authorships[3].raw_affiliation_strings	Universidad Nacional de Misiones Instituto de Biotecnologia Misiones Dra Maria Ebe Reca
has_content.pdf	True
has_content.grobid_xml	True
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062
open_access.oa_status	gold
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	Studies for lipase production ability of Aspergillus sp. strains from the Misiones rainforest of Paranaense.
has_fulltext	True
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T14256
primary_topic.field.id	https://openalex.org/fields/11
primary_topic.field.display_name	Agricultural and Biological Sciences
primary_topic.score	0.9693999886512756
primary_topic.domain.id	https://openalex.org/domains/1
primary_topic.domain.display_name	Life Sciences
primary_topic.subfield.id	https://openalex.org/subfields/1107
primary_topic.subfield.display_name	Forestry
primary_topic.display_name	Agricultural and Food Sciences
related_works	https://openalex.org/W4390762510, https://openalex.org/W2376067381, https://openalex.org/W2032677508, https://openalex.org/W1594336987, https://openalex.org/W2363056092, https://openalex.org/W1981133176, https://openalex.org/W2030231905, https://openalex.org/W2087148533, https://openalex.org/W2067146729, https://openalex.org/W1643869924
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.22541/au.169779096.64649062/v1
best_oa_location.is_oa	True
best_oa_location.source
best_oa_location.license
best_oa_location.pdf_url	https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062
best_oa_location.version	acceptedVersion
best_oa_location.raw_type	posted-content
best_oa_location.license_id
best_oa_location.is_accepted	True
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.22541/au.169779096.64649062/v1
primary_location.id	doi:10.22541/au.169779096.64649062/v1
primary_location.is_oa	True
primary_location.source
primary_location.license
primary_location.pdf_url	https://www.authorea.com/doi/pdf/10.22541/au.169779096.64649062
primary_location.version	acceptedVersion
primary_location.raw_type	posted-content
primary_location.license_id
primary_location.is_accepted	True
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.22541/au.169779096.64649062/v1
publication_date	2023-10-20
publication_year	2023
referenced_works_count	0
abstract_inverted_index.%	86
abstract_inverted_index.A	122
abstract_inverted_index.B	89
abstract_inverted_index.U	120
abstract_inverted_index.a	30, 116, 155
abstract_inverted_index.2%	145
abstract_inverted_index.38	207
abstract_inverted_index.80	85
abstract_inverted_index.an	58, 198
abstract_inverted_index.as	90
abstract_inverted_index.at	164, 176
abstract_inverted_index.by	3
abstract_inverted_index.in	66, 73, 151
abstract_inverted_index.it	99
abstract_inverted_index.of	5, 28, 32, 47, 61, 64, 68, 77, 118, 144, 167, 193
abstract_inverted_index.on	50, 94
abstract_inverted_index.pH	180
abstract_inverted_index.to	130, 159, 178
abstract_inverted_index.054	108
abstract_inverted_index.133	119
abstract_inverted_index.LBM	107
abstract_inverted_index.The	0, 53, 170, 190
abstract_inverted_index.and	25, 39, 87, 92, 173
abstract_inverted_index.are	9, 14, 26
abstract_inverted_index.for	186
abstract_inverted_index.gel	192
abstract_inverted_index.out	57
abstract_inverted_index.sp.	106
abstract_inverted_index.the	6, 17, 45, 62, 69, 74, 95, 103, 110, 131, 139, 142, 148, 160, 165, 168, 194, 202
abstract_inverted_index.was	100, 206
abstract_inverted_index.free	40
abstract_inverted_index.have	21
abstract_inverted_index.high	174
abstract_inverted_index.into	16
abstract_inverted_index.kDa.	208
abstract_inverted_index.make	182
abstract_inverted_index.many	48
abstract_inverted_index.mass	205
abstract_inverted_index.test	125
abstract_inverted_index.that	13, 102, 127
abstract_inverted_index.them	44
abstract_inverted_index.with	83, 115
abstract_inverted_index.work	55
abstract_inverted_index.Among	138
abstract_inverted_index.These	19
abstract_inverted_index.Tween	84
abstract_inverted_index.Using	79
abstract_inverted_index.based	93
abstract_inverted_index.broad	22
abstract_inverted_index.fatty	41
abstract_inverted_index.focus	46
abstract_inverted_index.found	126
abstract_inverted_index.fungi	4, 67
abstract_inverted_index.genus	7, 70
abstract_inverted_index.mL-1.	121
abstract_inverted_index.their	51
abstract_inverted_index.these	183
abstract_inverted_index.total	117
abstract_inverted_index.where	201
abstract_inverted_index.acids,	42
abstract_inverted_index.adding	128
abstract_inverted_index.lipase	112, 135, 152
abstract_inverted_index.making	43
abstract_inverted_index.medium	133
abstract_inverted_index.mostly	10
abstract_inverted_index.showed	197
abstract_inverted_index.strain	196
abstract_inverted_index.values	181
abstract_inverted_index.capable	27
abstract_inverted_index.carried	56
abstract_inverted_index.culture	132
abstract_inverted_index.enzymes	12, 184
abstract_inverted_index.esters,	38
abstract_inverted_index.highest	111, 149
abstract_inverted_index.initial	161
abstract_inverted_index.isolate	104
abstract_inverted_index.lipases	1, 20, 65
abstract_inverted_index.lipids,	33
abstract_inverted_index.neutral	177
abstract_inverted_index.optimal	171
abstract_inverted_index.present	54
abstract_inverted_index.studies	49
abstract_inverted_index.trials.	169
abstract_inverted_index.variety	31
abstract_inverted_index.various	187
abstract_inverted_index.Misiones	75
abstract_inverted_index.activity	136, 162, 172
abstract_inverted_index.addition	143
abstract_inverted_index.alkaline	179
abstract_inverted_index.analysis	60, 97
abstract_inverted_index.capacity	114
abstract_inverted_index.compared	158
abstract_inverted_index.fivefold	156
abstract_inverted_index.increase	150, 157
abstract_inverted_index.isolated	72
abstract_inverted_index.observed	163
abstract_inverted_index.produced	2
abstract_inverted_index.profile,	200
abstract_inverted_index.reaching	154
abstract_inverted_index.secreted	15
abstract_inverted_index.selected	195
abstract_inverted_index.suitable	185
abstract_inverted_index.zymogram	191
abstract_inverted_index.activity,	153
abstract_inverted_index.beginning	166
abstract_inverted_index.detection	81
abstract_inverted_index.enzymatic	199
abstract_inverted_index.evaluated	140
abstract_inverted_index.exhibited	109
abstract_inverted_index.including	34
abstract_inverted_index.increased	134
abstract_inverted_index.molecular	204
abstract_inverted_index.rhodamine	88
abstract_inverted_index.stability	175
abstract_inverted_index.substrate	23
abstract_inverted_index.conducted,	98
abstract_inverted_index.determined	101
abstract_inverted_index.production	63, 113
abstract_inverted_index.rainforest	76
abstract_inverted_index.techniques	82
abstract_inverted_index.tributyrin	129, 146
abstract_inverted_index.Aspergillus	8, 71, 105
abstract_inverted_index.Paranaense.	78
abstract_inverted_index.cholesterol	37
abstract_inverted_index.exploratory	59
abstract_inverted_index.hydrolyzing	29
abstract_inverted_index.protein’s	203
abstract_inverted_index.qualitative	80
abstract_inverted_index.specificity	24
abstract_inverted_index.statistical	124
abstract_inverted_index.substrates,	91
abstract_inverted_index.(P<0.05).	137
abstract_inverted_index.demonstrated	147
abstract_inverted_index.environment.	18
abstract_inverted_index.quantitative	96
abstract_inverted_index.applications.	52, 189
abstract_inverted_index.extracellular	11
abstract_inverted_index.phospholipids,	36
abstract_inverted_index.triglycerides,	35
abstract_inverted_index.Plackett-Burman	123
abstract_inverted_index.concentrations,	141
abstract_inverted_index.biotechnological	188
cited_by_percentile_year
countries_distinct_count	1
institutions_distinct_count	4
citation_normalized_percentile.value	0.22656128
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False