SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cells Article Swipe
Péter István Kulcsár
,
András Tálas
,
Zoltán Ligeti
,
Sarah Krausz
,
Ervin Welker
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.05.27.493683
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.05.27.493683
Several advances have been made to SpCas9, the most widely used CRISPR/Cas genome editing tool, to reduce its unwanted off-target effects. The most promising approach is the development of increased fidelity nuclease (IFN) variants of SpCas9, however, their fidelity has increased at the cost of reduced activity. SuperFi-Cas9 has been developed recently, and it has been described as a next-generation high fidelity SpCas9 variant, free from the drawbacks of the first-generation IFNs. In this study, we characterized the on-target activity and the off-target propensity of SuperFi-Cas9 in mammalian cells comparing it to first-generation IFNs. SuperFi-Cas9 demonstrated strongly reduced activity but exceptionally high fidelity exhibiting features that are in many aspects similar to those of the first-generation variants, such as evo- and HeFSpCas9. When combined with ABE8e, SuperFi-Cas9 produced DNA editing with high activity rate as well as high specificity by reducing both bystander and SpCas9-dependent off-target base editing.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2022.05.27.493683
- https://www.biorxiv.org/content/biorxiv/early/2022/07/11/2022.05.27.493683.full.pdf
- OA Status
- green
- Cited By
- 6
- References
- 35
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4281902560
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4281902560Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2022.05.27.493683Digital Object Identifier
- Title
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SuperFi-Cas9 exhibits extremely high fidelity but reduced activity in mammalian cellsWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-05-27Full publication date if available
- Authors
-
Péter István Kulcsár, András Tálas, Zoltán Ligeti, Sarah Krausz, Ervin WelkerList of authors in order
- Landing page
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https://doi.org/10.1101/2022.05.27.493683Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2022/07/11/2022.05.27.493683.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2022/07/11/2022.05.27.493683.full.pdfDirect OA link when available
- Concepts
-
Genome editing, Cas9, CRISPR, Medicine, Nuclease, Fidelity, High fidelity, Computational biology, Computer science, DNA, Genetics, Gene, Biology, Telecommunications, Engineering, Electrical engineeringTop concepts (fields/topics) attached by OpenAlex
- Cited by
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6Total citation count in OpenAlex
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2023: 1, 2022: 5Per-year citation counts (last 5 years)
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35Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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