Supplementary figure 4 from Decoy Receptor DcR1 Is Induced in a p50/Bcl3–Dependent Manner and Attenuates the Efficacy of Temozolomide Article Swipe

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Clonogenic assay Chemistry Temozolomide Transfection Apoptosis Ligand (biochemistry) Molecular biology Fas ligand Receptor Small interfering RNA Cancer research Pharmacology Medicine Biology Gene Programmed cell death Biochemistry Glioblastoma

Nassir M. Mansour , Giovanna M. Bernal , Longtao Wu , Clayton D. Crawley , Kirk E. Cahill , David J. Voce , Irina V. Balyasnikova , Wei Zhang , Rubén Spretz , Luis Nunez , Gustavo Larsen , Ralph R. Weichselbaum , Bakhtiar Yamini ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1158/0008-5472.22405617.v1 · OA: W4361848853

<p>Supplementary figure 4. A, clonogenic assay in U87 cells treated with Fas ligand (30 ng/ml) and/or FNAb (1 μg/ml). B, clonogenic assay in U87 cells transfected with the indicated siRNA following treatment with vehicle or Fas ligand (30 ng/ml). Data is normalized to vehicle for each si-RNA condition. C, clonogenic assay in U87 cells expressing empty vector (EV) or HA-DcR1 following treatment with Fas ligand (30 ng/ml). *, P < 0.05. D, tumor growth curves of U87 hindlimb xenografts (n = 5 mice per group) treated with the indicated combinations of TMZ and/or NPs carrying the indicated siRNA. Treatments were given as shown. *, P < 0.05, TMZ + NP-si-DcR1 vs. TMZ + NP-si-cntl.</p>