Supplementary Figure 5 from c-Src Modulates Estrogen-Induced Stress and Apoptosis in Estrogen-Deprived Breast Cancer Cells Article Swipe

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Trizol Oxidative stress HMOX1 Estrogen Apoptosis Chemistry Andrology MCF-7 Molecular biology Internal medicine Endocrinology Breast cancer Biology Cancer Medicine Human breast Gene Biochemistry Gene expression Enzyme Heme oxygenase RNA extraction Heme

Ping Fan , Obi L. Griffith , Fadeke A. Agboke , Pavana Anur , Xiaojun Zou , Russell E. McDaniel , Karen Creswell , Sung Hoon Kim , John A. Katzenellenbogen , Joe W. Gray , V. Craig Jordan ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1158/0008-5472.22397574.v1 · OA: W4362419645

<p>PDF file - 47K, 5A, The oxidative stress indicator HMOX1 expressed in wild-type MCF-7 cells after E2 treatment. Wild-type MCF-7 cells were cultured in E2 free medium for three days. Then, cells were plated in six-well plates. After one day, cells were treated with vehicle (0.1% EtOH) and E2 (10-9 mol/L) for different time points as indicated and the cells were harvested in TRIzol for real-time PCR. P<0.001, ** compared with control. 5B, The oxidative stress indicator HMOX1 expressed in long-term E2 deprived MCF-7:2A cells after E2 treatment. MCF-7:2A cells were plated in six-well plates. After one day, cells were treated with vehicle (0.1% EtOH) and E2 (10-9 mol/L) for different time points as indicated and the cells were harvested in TRIzol for real-time PCR. P<0.05, * compared with control. 5C, The oxidative stress indicator HMOX1 expressed in long-term E2 deprived MCF-7:5C cells after E2 treatment. MCF-7:5C cells were plated in six-well plates. After one day, cells were treated with vehicle (0.1% EtOH) and E2 (10-9 mol/L) for different time points as indicated and the cells were harvested in TRIzol for real-time PCR. P<0.05, * compared with control. P<0.001, ** compared with control.</p>