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Olaf Klingbeil , Damianos Skopelitis , Claudia Tonelli , Toyoki Yoshimoto , Aktan Alpsoy , Maria C. Panepinto , Francesca Minicozzi , Joseph R. Merrill , Amanda M. Cafiero , Disha Aggarwal , Suzanne Russo , Taehoon Ha , Osama E. Demerdash , Tse-Luen Wee , David L. Spector , Scott K. Lyons , David A. Tuveson , Paolo Cifani , Christopher R. Vakoc ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.1158/2159-8290.27938741

A, Western blot analysis of Cas9+ YAPC cells. B, Representative immunofluorescence (IF) images of YAP and DAPI following MARK2+3dKO in A549 cancer cells. scale bar = 30 µm. C, Comparison of mean immunofluorescence signal of YAP across the cell (A549) following MARK2+3dKO. P value was calculated using a two-tailed parametric t-test with Welch’s correction. n= 234 for Ctrl and n=155 for MARK2+3dKO cells were quantified. Data are shown as box plots with whiskers (5-95 percentile). D-E, Western blot analysis of MARK2 or MARK3 specific in-cell phosphorylation of Hippo pathway components. Substrates were labeled as described in Fig. 3D, and phosphorylation was identified by staining with thiophosphate ester-specific antibodies. Data are representative of two independent experiments. F, Coomassie stain of affinity purified recombinant Strep-SUMO tagged MARK2 (rMARK2) purified from insect cells. G, Western blot analysis of purified HA-CDC25C following treatment of Lambda phosphatase (L-PP) and phosphorylation using rMARK2.

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Western blot Molecular biology Phosphorylation Kinase Immunofluorescence DAPI Chemistry Staining Biology Antibody Cell biology Biochemistry Immunology Genetics Gene

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1158/2159-8290.27938741
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DOI: https://doi.org/10.1158/2159-8290.27938741

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Title: Supplementary Figure S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer

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Language: en

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Publication year: 2024

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Publication date: 2024-12-02

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Authors: Olaf Klingbeil, Damianos Skopelitis, Claudia Tonelli, Toyoki Yoshimoto, Aktan Alpsoy, Maria C. Panepinto, Francesca Minicozzi, Joseph R. Merrill, Amanda M. Cafiero, Disha Aggarwal, Suzanne Russo, Taehoon Ha, Osama E. Demerdash, Tse-Luen Wee, David L. Spector, Scott K. Lyons, David A. Tuveson, Paolo Cifani, Christopher R. Vakoc

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Landing page: https://doi.org/10.1158/2159-8290.27938741

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Concepts: Western blot, Molecular biology, Phosphorylation, Kinase, Immunofluorescence, DAPI, Chemistry, Staining, Biology, Antibody, Cell biology, Biochemistry, Immunology, Genetics, Gene

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abstract_inverted_index.independent	113
abstract_inverted_index.phosphatase	141
abstract_inverted_index.quantified.	64
abstract_inverted_index.recombinant	121
abstract_inverted_index.experiments.	114
abstract_inverted_index.percentile).	74
abstract_inverted_index.thiophosphate	105
abstract_inverted_index.Representative	9
abstract_inverted_index.ester-specific	106
abstract_inverted_index.representative	110
abstract_inverted_index.phosphorylation	85, 99, 144
abstract_inverted_index.rMARK2.</p>	146
abstract_inverted_index.immunofluorescence	10, 32
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	19
citation_normalized_percentile.value	0.28381302
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False