Supplementary Tables S1-S9 from Mutational Heterogeneity in <i>APC</i> and <i>KRAS</i> Arises at the Crypt Level and Leads to Polyclonality in Early Colorectal Tumorigenesis Article Swipe
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·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1158/1078-0432.22466628
Table S1. Summary of adenomas with multiple APC and KRAS somatic hits identified by next-generation sequencing. Table S2. Clinical and pathologic characteristics of patients analyzed in this manuscript. Table S3. Analysis of APC, KRAS and 5q loss using AmpliSeq and SNP array TableS4. Number of normal mucosa, adenomas, tumors and crypts analyzed per case. NM; normal mucosa. Table S5. Analytical sensibility of Light Cycler® 480 (Roche) and dynamic array (Fluidigm) to detect KRAS mutations. Table S6. In silico of the APC somatic hits observed by next-generaiton sequencing that were labelled as VUS. B, benign; N, neutral; D, damaging; P, Probably damaging. Table S7. APC analysis of adenomas and carcinomas in bulk biopsies and isolated crypts. The analysis of adjancent normal mucosa was not performed. Table S8. KRAS genotyping results of adenomas and carcinomas by digital and dynamic array (Fluidigm) in bulk biopsies and isolated crypts Table S9. Primers and probes used in this manuscript.
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Supplementary Tables S1-S9 from Mutational Heterogeneity in <i>APC</i> and <i>KRAS</i> Arises at the Crypt Level and Leads to Polyclonality in Early Colorectal TumorigenesisWork title
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enPrimary language
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2023Year of publication
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Mireia Gausachs, Ester Borràs, Kyle Chang, Sara González, Daniel Azuara, Axel Delgado Amador, Adriana López‐Doriga, F. Anthony San Lucas, Xavier Sanjuán, Maria J. Paules, Melissa W. Taggart, Gareth E. Davies, Erik A. Ehli, Jerry Fowler, Vı́ctor Moreno, Marta Pineda, Y. Nancy You, Patrick M. Lynch, Conxi Lázaro, Nicholas E. Navin, Paul Scheet, Ernest T. Hawk, Gabriel Capellá, Eduardo VilarList of authors in order
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| abstract_inverted_index.S8. | 125 |
| abstract_inverted_index.S9. | 146 |
| abstract_inverted_index.SNP | 40 |
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| abstract_inverted_index.per | 52 |
| abstract_inverted_index.the | 79 |
| abstract_inverted_index.was | 121 |
| abstract_inverted_index.APC, | 32 |
| abstract_inverted_index.KRAS | 9, 33, 72, 126 |
| abstract_inverted_index.VUS. | 91 |
| abstract_inverted_index.bulk | 110, 140 |
| abstract_inverted_index.hits | 11, 82 |
| abstract_inverted_index.loss | 36 |
| abstract_inverted_index.that | 87 |
| abstract_inverted_index.this | 26, 152 |
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| abstract_inverted_index.array | 41, 68, 137 |
| abstract_inverted_index.case. | 53 |
| abstract_inverted_index.using | 37 |
| abstract_inverted_index.Number | 43 |
| abstract_inverted_index.crypts | 50, 144 |
| abstract_inverted_index.detect | 71 |
| abstract_inverted_index.mucosa | 120 |
| abstract_inverted_index.normal | 45, 55, 119 |
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| abstract_inverted_index.silico | 77 |
| abstract_inverted_index.tumors | 48 |
| abstract_inverted_index.(Roche) | 65 |
| abstract_inverted_index.Primers | 147 |
| abstract_inverted_index.Summary | 2 |
| abstract_inverted_index.benign; | 93 |
| abstract_inverted_index.crypts. | 114 |
| abstract_inverted_index.digital | 134 |
| abstract_inverted_index.dynamic | 67, 136 |
| abstract_inverted_index.mucosa, | 46 |
| abstract_inverted_index.mucosa. | 56 |
| abstract_inverted_index.results | 128 |
| abstract_inverted_index.somatic | 10, 81 |
| abstract_inverted_index.AmpliSeq | 38 |
| abstract_inverted_index.Analysis | 30 |
| abstract_inverted_index.Clinical | 18 |
| abstract_inverted_index.Cycler® | 63 |
| abstract_inverted_index.Probably | 99 |
| abstract_inverted_index.TableS4. | 42 |
| abstract_inverted_index.adenomas | 4, 106, 130 |
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| abstract_inverted_index.analyzed | 24, 51 |
| abstract_inverted_index.biopsies | 111, 141 |
| abstract_inverted_index.isolated | 113, 143 |
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| abstract_inverted_index.multiple | 6 |
| abstract_inverted_index.neutral; | 95 |
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| abstract_inverted_index.adenomas, | 47 |
| abstract_inverted_index.adjancent | 118 |
| abstract_inverted_index.damaging. | 100 |
| abstract_inverted_index.damaging; | 97 |
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| abstract_inverted_index.sequencing. | 15 |
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| abstract_inverted_index.next-generation | 14 |
| abstract_inverted_index.manuscript.</p> | 153 |
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| institutions_distinct_count | 24 |
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