Supplementary Video S2 from Low-Dose Ionizing γ-Radiation Elicits the Extrusion of Neutrophil Extracellular Traps Article Swipe
Álvaro Teijeira
,
Saray Garasa
,
María C. Ochoa
,
Sandra Sánchez‐Gregorio
,
Gabriel Gomis
,
Carlos Luri‐Rey
,
Rafael Martínez‐Monge
,
Beatrice Pinci
,
Karmele Valencia
,
Belén Palencia
,
Benigno Barbés
,
Elixabet Bolaños
,
Arantza Azpilikueta
,
Marina García-Cardosa
,
Javier Burguete
,
Iñaki Eguren‐Santamaría
,
Eneko Garate-Soraluze
,
Pedro Berraondo
,
José Luis Perez‐Gracia
,
Carlos E. de Andrea
,
María E. Rodriguez-Ruiz
,
Ignacio Melero
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1158/1078-0432.27013525.v1
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1158/1078-0432.27013525.v1
Supplementary Video S2. Time-lapse confocal microscopy of co-cultures of tumor spheroids covered by radiation-induced NETs and cytotoxic lymphocytes. HT29 tumor cell-spheroids stained with CMRA (red) were cocultured or not with 2 Gy-irradiated neutrophils in the presence/absence of DNAse I to allow the presence of NETs (Sytox Green, green) around the spheroids. Cytotoxic immune cells (T or NK lymphocytes, blue) were pre-stained and cocultured with tumor spheroids for 4 hours before confocal microscopy acquisition. Time-lapse videos show a representative z-slice of the co-cultures over time (8 hours, 2,400× accelerated). As quantified in Figures 2 A, B and D, E, kinetically monitoring the tracks of the lymphocytes, data show evidence for the avoidance of the NETs in the migration paths.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1158/1078-0432.27013525.v1
- OA Status
- gold
- Related Works
- 10
- OpenAlex ID
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Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4402526191Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1158/1078-0432.27013525.v1Digital Object Identifier
- Title
-
Supplementary Video S2 from Low-Dose Ionizing γ-Radiation Elicits the Extrusion of Neutrophil Extracellular TrapsWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
-
2024-09-13Full publication date if available
- Authors
-
Álvaro Teijeira, Saray Garasa, María C. Ochoa, Sandra Sánchez‐Gregorio, Gabriel Gomis, Carlos Luri‐Rey, Rafael Martínez‐Monge, Beatrice Pinci, Karmele Valencia, Belén Palencia, Benigno Barbés, Elixabet Bolaños, Arantza Azpilikueta, Marina García-Cardosa, Javier Burguete, Iñaki Eguren‐Santamaría, Eneko Garate-Soraluze, Pedro Berraondo, José Luis Perez‐Gracia, Carlos E. de Andrea, María E. Rodriguez-Ruiz, Ignacio MeleroList of authors in order
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https://doi.org/10.1158/1078-0432.27013525.v1Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1158/1078-0432.27013525.v1Direct OA link when available
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Ionizing radiation, Neutrophil extracellular traps, Extrusion, Extracellular, Radiation, Irradiation, Radiochemistry, Chemistry, Physics, Materials science, Biology, Immunology, Biochemistry, Nuclear physics, Inflammation, Composite materialTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- Related works (count)
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10Other works algorithmically related by OpenAlex
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