Surface reactivity measurements as required for grouping and read-across: An advanced FRAS protocol Article Swipe
Arnaud Gandon
,
Kai Werle
,
Nicole Neubauer
,
Wendel Wohlleben
·
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1088/1742-6596/838/1/012033
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1088/1742-6596/838/1/012033
Oxidative stress is a widely accepted paradigm associated with different adverse outcomes of particulate matter, including nanomaterials. It has frequently been identified in in vitro and in vivo studies and different assays have been developed for this purpose. Here we describe a newly developed multi-dose protocol of the FRAS assay (Ferric Reduction Ability of Serum). The purpose of this SOP is the measurement of the surface reactivity of nanomaterials under physiological conditions. Antioxidative components as present in human blood serum (HBS) serve as reporter molecules. The assay separates the oxidative damage from the read-out of the reporter molecules. The results show significantly enhanced repeatability with better sensitivity towards low reactivity, enabling application of FRAS both to a rough grouping by reactive vs. passive nanomaterials and further to substantiation of read-across by enhanced resolution of the similarity between different nanoforms of the same substance.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1088/1742-6596/838/1/012033
- https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012033/pdf
- OA Status
- diamond
- Cited By
- 33
- References
- 13
- Related Works
- 10
- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W2621031125Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1088/1742-6596/838/1/012033Digital Object Identifier
- Title
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Surface reactivity measurements as required for grouping and read-across: An advanced FRAS protocolWork title
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articleOpenAlex work type
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enPrimary language
- Publication year
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2017Year of publication
- Publication date
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2017-06-01Full publication date if available
- Authors
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Arnaud Gandon, Kai Werle, Nicole Neubauer, Wendel WohllebenList of authors in order
- Landing page
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https://doi.org/10.1088/1742-6596/838/1/012033Publisher landing page
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https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012033/pdfDirect link to full text PDF
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diamondOpen access status per OpenAlex
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https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012033/pdfDirect OA link when available
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Nanomaterials, Reactivity (psychology), Oxidative stress, Protocol (science), In vivo, Chemistry, Repeatability, Resolution (logic), In vitro, Nanotechnology, Materials science, Computer science, Biology, Biochemistry, Medicine, Chromatography, Biotechnology, Pathology, Artificial intelligence, Alternative medicineTop concepts (fields/topics) attached by OpenAlex
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33Total citation count in OpenAlex
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2025: 2, 2024: 5, 2023: 1, 2022: 3, 2021: 10Per-year citation counts (last 5 years)
- References (count)
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13Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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