Switching the activity of Taq polymerase using clamp-like triplex aptamer structure Article Swipe
Yingxin Hu
,
Zhiyu Wang
,
Zhekun Chen
,
Linqiang Pan
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1093/nar/gkaa581
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1093/nar/gkaa581
In nature, allostery is the principal approach for regulating cellular processes and pathways. Inspired by nature, structure-switching aptamer-based nanodevices are widely used in artificial biotechnologies. However, the canonical aptamer structures in the nanodevices usually adopt a duplex form, which limits the flexibility and controllability. Here, a new regulating strategy based on a clamp-like triplex aptamer structure (CLTAS) was proposed for switching DNA polymerase activity via conformational changes. It was demonstrated that the polymerase activity could be regulated by either adjusting structure parameters or dynamic reactions including strand displacement or enzymatic digestion. Compared with the duplex aptamer structure, the CLTAS possesses programmability, excellent affinity and high discrimination efficiency. The CLTAS was successfully applied to distinguish single-base mismatches. The strategy expands the application scope of triplex structures and shows potential in biosensing and programmable nanomachines.
Related Topics
Concepts
Biology
Taq polymerase
Aptamer
Polymerase chain reaction
Clamp
Computational biology
Polymerase
Molecular biology
Genetics
Thermus aquaticus
DNA
Gene
Computer science
Computer vision
Clamping
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1093/nar/gkaa581
- OA Status
- gold
- Cited By
- 20
- References
- 52
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3041263149
All OpenAlex metadata
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- OpenAlex ID
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https://openalex.org/W3041263149Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1093/nar/gkaa581Digital Object Identifier
- Title
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Switching the activity of Taq polymerase using clamp-like triplex aptamer structureWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2020Year of publication
- Publication date
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2020-06-27Full publication date if available
- Authors
-
Yingxin Hu, Zhiyu Wang, Zhekun Chen, Linqiang PanList of authors in order
- Landing page
-
https://doi.org/10.1093/nar/gkaa581Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://doi.org/10.1093/nar/gkaa581Direct OA link when available
- Concepts
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Biology, Taq polymerase, Aptamer, Polymerase chain reaction, Clamp, Computational biology, Polymerase, Molecular biology, Genetics, Thermus aquaticus, DNA, Gene, Computer science, Computer vision, ClampingTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
20Total citation count in OpenAlex
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2025: 3, 2024: 6, 2023: 2, 2022: 4, 2021: 4Per-year citation counts (last 5 years)
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52Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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