Tandem Probe Analysis Mode for Synchrotron XFM: Doubling Throughput Capacity Article Swipe
Casey L. Doolette
,
Daryl L. Howard
,
Nader Afshar
,
Cameron M. Kewish
,
David Paterson
,
Jianyin Huang
,
Stefan Wagner
,
Jakob Santner
,
Walter W. Wenzel
,
Tom Raimondo
,
Alexander T. De Vries Van Leeuwen
,
Lei Hou
,
Frederik van der Bom
,
Zhe Weng
,
Peter M. Kopittke
,
Enzo Lombi
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.1c04255
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.1c04255
Synchrotron-based X-ray fluorescence microscopy (XFM) analysis is a powerful technique that can be used to visualize elemental distributions across a broad range of sample types. Compared to conventional mapping techniques such as laser ablation inductively coupled plasma mass spectrometry or benchtop XFM, synchrotron-based XFM provides faster and more sensitive analyses. However, access to synchrotron XFM beamlines is highly competitive, and as a result, these beamlines are often oversubscribed. Therefore, XFM experiments that require many large samples to be scanned can penalize beamline throughput. Our study was largely driven by the need to scan large gels (170 cm2) using XFM without decreasing beamline throughput. We describe a novel approach for acquiring two sets of XFM data using two fluorescence detectors in tandem; essentially performing two separate experiments simultaneously. We measured the effects of tandem scanning on beam quality by analyzing a range of contrasting samples downstream while simultaneously scanning different gel materials upstream. The upstream gels were thin (99%) transmittance with minimal (<3%) flux variation during raster scanning, whereas the other gels had a substantial effect on the beam focus. For the first time, we have (1) used XFM for mapping analytes in large DGTs and (2) developed a tandem probe analysis mode for synchrotron-based XFM, effectively doubling throughput. The novel tandem probe analysis mode described here is of broad applicability across many XFM beamlines as it could be used for future experiments where any uniform, highly transmissive sample could be analyzed upstream in the "background"of downstream samples.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acs.analchem.1c04255
- OA Status
- hybrid
- Cited By
- 7
- References
- 35
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4220715837
All OpenAlex metadata
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https://openalex.org/W4220715837Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/acs.analchem.1c04255Digital Object Identifier
- Title
-
Tandem Probe Analysis Mode for Synchrotron XFM: Doubling Throughput CapacityWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-03-11Full publication date if available
- Authors
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Casey L. Doolette, Daryl L. Howard, Nader Afshar, Cameron M. Kewish, David Paterson, Jianyin Huang, Stefan Wagner, Jakob Santner, Walter W. Wenzel, Tom Raimondo, Alexander T. De Vries Van Leeuwen, Lei Hou, Frederik van der Bom, Zhe Weng, Peter M. Kopittke, Enzo LombiList of authors in order
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https://doi.org/10.1021/acs.analchem.1c04255Publisher landing page
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://doi.org/10.1021/acs.analchem.1c04255Direct OA link when available
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Synchrotron, Chemistry, Beamline, Microscopy, Analytical Chemistry (journal), Optics, Chromatography, Beam (structure), PhysicsTop concepts (fields/topics) attached by OpenAlex
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7Total citation count in OpenAlex
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2025: 3, 2024: 1, 2023: 3Per-year citation counts (last 5 years)
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35Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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