Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes Article Swipe
Chloé Roffay
,
Juan Manuel GARCIA ARCOS
,
Pierrik Chapuis
,
Javier López‐Andarias
,
Falk Schneider
,
Adai Colom
,
Caterina Tomba
,
Ilaria Di Meglio
,
Valentin Dunsing
,
Stefan Matile
,
Aurélien Roux
,
Vincent Mercier
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.09.28.509885
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.09.28.509885
Measuring forces within living cells remains a technical challenge. We developed hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing and can report on membrane tension. Here, we describe technical optimization of the probe imaging, and diverse characterizations in various biological and in vitro systems. We provide a guideline to measure biophysical parameters of cellular membranes by FLIM microscopy with Flipper probes, providing evidences that flippers can report long range forces in cells, tissues and organs i .
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2022.09.28.509885
- https://www.biorxiv.org/content/biorxiv/early/2023/01/31/2022.09.28.509885.full.pdf
- OA Status
- green
- Cited By
- 23
- References
- 54
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4298091855
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4298091855Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2022.09.28.509885Digital Object Identifier
- Title
-
Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probesWork title
- Type
-
preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-09-30Full publication date if available
- Authors
-
Chloé Roffay, Juan Manuel GARCIA ARCOS, Pierrik Chapuis, Javier López‐Andarias, Falk Schneider, Adai Colom, Caterina Tomba, Ilaria Di Meglio, Valentin Dunsing, Stefan Matile, Aurélien Roux, Vincent MercierList of authors in order
- Landing page
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https://doi.org/10.1101/2022.09.28.509885Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2023/01/31/2022.09.28.509885.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2023/01/31/2022.09.28.509885.full.pdfDirect OA link when available
- Concepts
-
Mechanosensitive channels, Fluorescence microscope, Microscopy, Fluorescence, Biophysics, Membrane, Live cell imaging, Fluorescence-lifetime imaging microscopy, Nanotechnology, Biology, Materials science, Optics, Physics, Ion channel, Cell, Biochemistry, ReceptorTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
23Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 7, 2024: 9, 2023: 7Per-year citation counts (last 5 years)
- References (count)
-
54Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.evidences | 68 |
| abstract_inverted_index.guideline | 53 |
| abstract_inverted_index.membranes | 60 |
| abstract_inverted_index.providing | 67 |
| abstract_inverted_index.technical | 8, 34 |
| abstract_inverted_index.biological | 45 |
| abstract_inverted_index.challenge. | 9 |
| abstract_inverted_index.microscopy | 63 |
| abstract_inverted_index.parameters | 57 |
| abstract_inverted_index.biophysical | 56 |
| abstract_inverted_index.fluorescent | 14 |
| abstract_inverted_index.hydrophobic | 12 |
| abstract_inverted_index.fluorescence | 19 |
| abstract_inverted_index.optimization | 35 |
| abstract_inverted_index.mechanosensing | 13 |
| abstract_inverted_index.characterizations | 42 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 98 |
| corresponding_author_ids | https://openalex.org/A5026059999, https://openalex.org/A5017325892 |
| countries_distinct_count | 4 |
| institutions_distinct_count | 12 |
| corresponding_institution_ids | https://openalex.org/I114457229, https://openalex.org/I4210129591 |
| citation_normalized_percentile.value | 0.88374182 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |