The effects of L-Carnitine as nutrigenomic factor on gene expression under various conditions Article Swipe
HINTERGRUND: L-Carnitin hat als wichtiger nutrigenomischer Metabolit, einen enormen Einfluss auf die Genexpression. ZIELE: Das Ziel dieser Arbeit ist die Herstellung eines Vergleiches zwischen Genexpressionen der Gene BCOX1, TBDN100, STK4 und LMNB1 in zwei verschiedenen Zelllinien. Diese Zelllinien stammen in unserem Fall aus dem Muskel und aus der Leber und wurden mit diversen L-Carnitin-Zusätzen kultiviert. Zum einen haben wir dafür die humane Leberzelllinie WRL68 ausgewählt, die morphologisch Hepatozyten sowie primären Leberzellen entspricht und somit die metabolischen Funktionen der Leber repräsentiert. Zum anderen wurde dazu vergleichend die SKMC Zelllinie analysiert, die ursprünglich von primaren humanen Skelettmuskelzellen isoliert wurde (e.g. Musculus pectoralis major) und somit optimal die Speicherfunktion der Skelettmuskelzellen darzustellen vermag. MATERIAL/METHODEN: Alle Zellen, welche für den Zusatz von L-Carnitin vorgesehen waren, wurden vorher für drei Tage in einem L-Carnitin-freien Medium, mit 10%-igem dialysierten FCS kultiviert. Anschließend wurde die gewünschte Menge L-Carnitin beigefügt und die Zellen für weitere vier Stunden kultiviert. Die mRNA wurde extrahiert und in cDNA transkribiert. Als Methode der Wahl, zur Feststellung der Mengen an exprimierter Gene, diente die qPCR. ERGEBNISSE: Alle untersuchten Gene zeigten einen signifikanten Einfluss von LCarnitin bezüglich ihrer Amplifikationsrate. Es gab einen signifikanten Unterschied zwischen der Genexpression von TBDN100 in den WRL68 Leberzellen und den SKMC Zellen. Hervorzuheben ist, dass die ansteigenden Mengen der L-CarnitinZugabe in den SKMC die Genexpression von TBDN100 entsprechened herunter regulieren konnte. Ein weiterer wichtiger Aspekt ist die signifikant positive Korrelation zwischen BCOX1 und der 40µM L-Carnitinsupplementation als Kultivationsbedingung in der Zellkultur. SCHLUSSFOLGERUNG: Unterschiedliche Konzentrationsstufen von L-Carnitin in den Leber- und Muskelzellen beeinflussen den Metabolismus des Menschen generell IX und die Genexpression der vier ausgewählten Gene im Speziellen. TBDN100 hat sich als das variabelste Gen im Vergleich zwischen Leber- und Skelettmuskelzellen herausgestellt. Die Expression dieses Gens spielt eine wichtige Rolle bei der Modifikation der Proteine nach der Translation und der Azetylierung von Histonen.
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The effects of L-Carnitine as nutrigenomic factor on gene expression under various conditionsWork title
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articleOpenAlex work type
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dePrimary language
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2019Year of publication
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2019-01-01Full publication date if available
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Magdalena SteinerList of authors in order
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Molecular biology, BiologyTop concepts (fields/topics) attached by OpenAlex
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