The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay Article Swipe
YOU?
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· 2024
· Open Access
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· DOI: https://doi.org/10.3390/bios14010030
A colloidal gold-based lateral flow immunoassay was developed for the rapid quantitative detection of Cystatin-C in serum and whole blood. This device has an assay time of 15 min, making it a convenient point-of-care diagnostic tool. The device has a quantification range spanning from 0.5 to 7.5 µg/mL, with a lower limit of detection at 0.18 µg/mL. To validate its accuracy, the test was compared to a standard nephelometric immunoassay, and the results exhibited a robust linear correlation with an adjusted r2 value of 0.95. Furthermore, the device demonstrates satisfactory levels of analytical performance in terms of precision, sensitivity, and interference, indicating its potential for precise Cystatin-C quantification, particularly in renal-failure patients. Notably, the Cystatin-C-LFA device also demonstrates satisfactory stability, as a 30-day accelerated stability study at 50 °C showed no change in the device performance, indicating a long shelf life for the product when stored at room temperature.
Related Topics
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/bios14010030
- https://www.mdpi.com/2079-6374/14/1/30/pdf?version=1704682883
- OA Status
- gold
- Cited By
- 12
- References
- 31
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4390722330
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4390722330Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/bios14010030Digital Object Identifier
- Title
-
The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow ImmunoassayWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-01-08Full publication date if available
- Authors
-
Santosh Kumar Bikkarolla, Kavipriya Venkatesan, Yeddula Rebecca Revathy, Sowmya Parameswaran, Subramanian Krishnakumar, Dhananjaya DendukuriList of authors in order
- Landing page
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https://doi.org/10.3390/bios14010030Publisher landing page
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https://www.mdpi.com/2079-6374/14/1/30/pdf?version=1704682883Direct link to full text PDF
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://www.mdpi.com/2079-6374/14/1/30/pdf?version=1704682883Direct OA link when available
- Concepts
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Immunoassay, Cystatin C, Chromatography, Chemistry, Medicine, Internal medicine, Creatinine, Immunology, AntibodyTop concepts (fields/topics) attached by OpenAlex
- Cited by
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12Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 10, 2024: 2Per-year citation counts (last 5 years)
- References (count)
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31Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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