The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate Article Swipe

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Rosa Merlo , Diego Caprioglio , Michele Cillo , Anna Valenti , Rosanna Mattossovich , Castrese Morrone , Alberto Massarotti , Franca Rossi , Riccardo Miggiano , Antonio Leonardi , Alberto Minassi , Giuseppe Perugino ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.6084/m9.figshare.13168111

SNAP-tag^® is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates. Although commercially available or ad hoc produced, their synthesis and purification are necessary, increasing time and costs. To address this limitation, here we suggest a revision of this methodology, by performing a chemo-enzymatic approach, by using a BG-substrate containing an azide group appropriately distanced by a spacer from the benzyl ring. The SNAP-tag^® and its relative thermostable version (SsOGT-H⁵) proved to be very active on this substrate. The stability of these tags upon enzymatic reaction makes possible the exposition to the solvent of the azide-moiety linked to the catalytic cysteine, compatible for the subsequent conjugation with DBCO-derivatives by azide-alkyne Huisgen cycloaddition. Our studies propose a strengthening and an improvement in terms of biotechnological applications for this self-labelling protein-tag.

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Azide Substrate (aquarium) Snap Computer science Chemistry Nanotechnology Materials science Combinatorial chemistry Operating system Biology Organic chemistry Ecology

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Type: dataset
Language: en
Landing Page: https://doi.org/10.6084/m9.figshare.13168111
OA Status: gold
Related Works: 10
OpenAlex ID: https://openalex.org/W4394451739

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OpenAlex ID: https://openalex.org/W4394451739

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DOI: https://doi.org/10.6084/m9.figshare.13168111

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Title: The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate

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Language: en

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Publication year: 2020

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Publication date: 2020-01-01

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Authors: Rosa Merlo, Diego Caprioglio, Michele Cillo, Anna Valenti, Rosanna Mattossovich, Castrese Morrone, Alberto Massarotti, Franca Rossi, Riccardo Miggiano, Antonio Leonardi, Alberto Minassi, Giuseppe Perugino

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Landing page: https://doi.org/10.6084/m9.figshare.13168111

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OA URL: https://doi.org/10.6084/m9.figshare.13168111

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Concepts: Azide, Substrate (aquarium), Snap, Computer science, Chemistry, Nanotechnology, Materials science, Combinatorial chemistry, Operating system, Biology, Organic chemistry, Ecology

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Cited by: 0

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Related works (count): 10

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