Thermal denaturation of DNA from bromodeoxyuridine substituted cells Article Swipe
Related Concepts
Ionic strength
Bromodeoxyuridine
DNA
Biology
Ionic bonding
Denaturation (fissile materials)
Ion
Nucleic Acid Denaturation
Biophysics
Molecular biology
Biochemistry
Nuclear chemistry
Cell growth
Chemistry
Aqueous solution
Organic chemistry
Base sequence
Bryan R. Cullen
,
Michael D. Bick
·
YOU?
·
· 1976
· Open Access
·
· DOI: https://doi.org/10.1093/nar/3.1.49
· OA: W1988410861
YOU?
·
· 1976
· Open Access
·
· DOI: https://doi.org/10.1093/nar/3.1.49
· OA: W1988410861
The thermal denaturation of DNA from cell lines extensively substituted with bromodeoxyuridine has been examined spectrophotometrically over a wide range in ionic strength and by thermal elution from hydroxyapatite columns. BrdU substitution stabliizes DNA at all ionic strengths between 7.5 mM and 1350 mM potassium ion concentration, although a plot of log ionic strength vs Tm deviates from linearity above 150 mM. This nonlinearity is most pronounced with BrdU-substituted DNAs, resulting in a lowered delta Tm between unsubstituted and substituted DNA with increasing ionic strength. DMSO is shown to decrease the Tm of both unsubstituted and BrdU-substituted DNA equally, at a rate of .5 degrees C per 1% DMSO.
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