Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser Article Swipe

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Femtosecond Self-healing hydrogels Laser Materials science Nanorod Biomedical engineering Tissue engineering Nanotechnology Optoelectronics Optics Medicine Physics Polymer chemistry

Kolin C. Hribar , Kyle Meggs , Justin Liu , Wei Zhu , Xin Qu , Shaochen Chen ·

YOU? · · 2015 · Open Access · · DOI: https://doi.org/10.1038/srep17203 · OA: W2416816599

We report a methodology for three-dimensional (3D) cell patterning in a hydrogel in situ . Gold nanorods within a cell-encapsulating collagen hydrogel absorb a focused near-infrared femtosecond laser beam, locally denaturing the collagen and forming channels, into which cells migrate, proliferate and align in 3D. Importantly, pattern resolution is tunable based on writing speed and laser power and high cell viability (>90%) is achieved using higher writing speeds and lower laser intensities. Overall, this patterning technique presents a flexible direct-write method that is applicable in tissue engineering systems where 3D alignment is critical (such as vascular, neural, cardiac and muscle tissue).