TiO2 nanoparticle synthesis, characterization and application to shoot regeneration of water hyssop (Bacopa monnieri L. Pennel) in vitro Article Swipe
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· 2022
· Open Access
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· DOI: https://doi.org/10.1080/10520295.2022.2087236
· OA: W4283746361
Water hyssop (<i>Bacopa monnieri</i> L. Pennel) is a medicinal aquatic herb used to treat diseases in South Asia. Various regeneration protocols have been developed or modified in vitro to ensure the availability of biomass and secondary metabolites of <i>Bacopa</i>. We applied hydrothermally treated titanium dioxide (TiO<sub>2</sub>) nanoparticles (NPs) (TiO<sub>2</sub>-NPs) at different concentrations. Three explants, distal portion of half leaf (DPHL), proximal portion of half leaf (PPHL) and full leaf (FL), were used to evaluate response to TiO<sub>2</sub>. Regeneration from the three explants in vitro was similar except for shoot length. Application of TiO<sub>2</sub>-NPs exerted significant, but variable, effects on all parameters except percentage of shoot formation, which was 100%. Interactive effects of explant and TiO<sub>2</sub>-NPs exhibited significant, but variable, effects on fresh weight and percentage of callus formation. All explants produced more shoots using TiO<sub>2</sub>-NPs compared to control treatments. DPHL explants with application of 8 mg/l TiO<sub>2</sub> produced more shoots than controls. Similarly, FL explant treated with 2 mg/l TiO<sub>2</sub>-NPs produced more shoots/explant than controls. All concentrations of TiO<sub>2</sub>-NPs produced significantly longer shoots compared to controls. Increased elongation of shoots justifies use of TiO<sub>2</sub>-NPs for propagation of plants in vitro during acclimatization. Use of TiO<sub>2</sub>-NPs for rapid elongation of shoots ultimately fosters survival of plants.
