Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production Article Swipe
Suvi T. Häkkinen
,
Lauri Reuter
,
Ninni Nuorti
,
Jussi J. Joensuu
,
Heiko Rischer
,
Anneli Ritala
·
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.3389/fpls.2018.00045
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.3389/fpls.2018.00045
Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein-Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named 'Hulk,' produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43-55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fpls.2018.00045
- https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdf
- OA Status
- gold
- Cited By
- 46
- References
- 29
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2793825735
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2793825735Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fpls.2018.00045Digital Object Identifier
- Title
-
Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein ProductionWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2018Year of publication
- Publication date
-
2018-01-26Full publication date if available
- Authors
-
Suvi T. Häkkinen, Lauri Reuter, Ninni Nuorti, Jussi J. Joensuu, Heiko Rischer, Anneli RitalaList of authors in order
- Landing page
-
https://doi.org/10.3389/fpls.2018.00045Publisher landing page
- PDF URL
-
https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdfDirect OA link when available
- Concepts
-
Recombinant DNA, clone (Java method), Green fluorescent protein, Fusion protein, Biotechnology, Biomass (ecology), Food science, Hydrophobin, Cell culture, Biology, Transgene, Chemistry, Biochemistry, Gene, Agronomy, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
46Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 6, 2024: 7, 2023: 8, 2022: 9, 2021: 7Per-year citation counts (last 5 years)
- References (count)
-
29Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2793825735 |
|---|---|
| doi | https://doi.org/10.3389/fpls.2018.00045 |
| ids.doi | https://doi.org/10.3389/fpls.2018.00045 |
| ids.mag | 2793825735 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/29434617 |
| ids.openalex | https://openalex.org/W2793825735 |
| fwci | 3.28878088 |
| type | article |
| title | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| awards[0].id | https://openalex.org/G7713314246 |
| awards[0].funder_id | https://openalex.org/F4320321108 |
| awards[0].display_name | |
| awards[0].funder_award_id | 252442 |
| awards[0].funder_display_name | Academy of Finland |
| biblio.issue | |
| biblio.volume | 9 |
| biblio.last_page | 45 |
| biblio.first_page | 45 |
| topics[0].id | https://openalex.org/T12856 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 1.0 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1305 |
| topics[0].subfield.display_name | Biotechnology |
| topics[0].display_name | Transgenic Plants and Applications |
| topics[1].id | https://openalex.org/T10240 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9987000226974487 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Plant tissue culture and regeneration |
| topics[2].id | https://openalex.org/T11426 |
| topics[2].field.id | https://openalex.org/fields/11 |
| topics[2].field.display_name | Agricultural and Biological Sciences |
| topics[2].score | 0.9776999950408936 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1110 |
| topics[2].subfield.display_name | Plant Science |
| topics[2].display_name | Genetically Modified Organisms Research |
| funders[0].id | https://openalex.org/F4320321108 |
| funders[0].ror | https://ror.org/05k73zm37 |
| funders[0].display_name | Academy of Finland |
| is_xpac | False |
| apc_list.value | 2950 |
| apc_list.currency | USD |
| apc_list.value_usd | 2950 |
| apc_paid.value | 2231 |
| apc_paid.currency | EUR |
| apc_paid.value_usd | 2406 |
| concepts[0].id | https://openalex.org/C40767141 |
| concepts[0].level | 3 |
| concepts[0].score | 0.7675395011901855 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q285697 |
| concepts[0].display_name | Recombinant DNA |
| concepts[1].id | https://openalex.org/C81089528 |
| concepts[1].level | 3 |
| concepts[1].score | 0.5395334362983704 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q5134986 |
| concepts[1].display_name | clone (Java method) |
| concepts[2].id | https://openalex.org/C142613039 |
| concepts[2].level | 3 |
| concepts[2].score | 0.5309215188026428 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q272983 |
| concepts[2].display_name | Green fluorescent protein |
| concepts[3].id | https://openalex.org/C123894998 |
| concepts[3].level | 4 |
| concepts[3].score | 0.5239855647087097 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q425158 |
| concepts[3].display_name | Fusion protein |
| concepts[4].id | https://openalex.org/C150903083 |
| concepts[4].level | 1 |
| concepts[4].score | 0.48851120471954346 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q7108 |
| concepts[4].display_name | Biotechnology |
| concepts[5].id | https://openalex.org/C115540264 |
| concepts[5].level | 2 |
| concepts[5].score | 0.48457157611846924 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q2945560 |
| concepts[5].display_name | Biomass (ecology) |
| concepts[6].id | https://openalex.org/C31903555 |
| concepts[6].level | 1 |
| concepts[6].score | 0.4612748622894287 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q1637030 |
| concepts[6].display_name | Food science |
| concepts[7].id | https://openalex.org/C2778104808 |
| concepts[7].level | 3 |
| concepts[7].score | 0.4381014406681061 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q24745308 |
| concepts[7].display_name | Hydrophobin |
| concepts[8].id | https://openalex.org/C81885089 |
| concepts[8].level | 2 |
| concepts[8].score | 0.43764230608940125 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q189082 |
| concepts[8].display_name | Cell culture |
| concepts[9].id | https://openalex.org/C86803240 |
| concepts[9].level | 0 |
| concepts[9].score | 0.4372156262397766 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[9].display_name | Biology |
| concepts[10].id | https://openalex.org/C102230213 |
| concepts[10].level | 3 |
| concepts[10].score | 0.4205589294433594 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q430117 |
| concepts[10].display_name | Transgene |
| concepts[11].id | https://openalex.org/C185592680 |
| concepts[11].level | 0 |
| concepts[11].score | 0.34225931763648987 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[11].display_name | Chemistry |
| concepts[12].id | https://openalex.org/C55493867 |
| concepts[12].level | 1 |
| concepts[12].score | 0.2981182932853699 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[12].display_name | Biochemistry |
| concepts[13].id | https://openalex.org/C104317684 |
| concepts[13].level | 2 |
| concepts[13].score | 0.1593596339225769 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[13].display_name | Gene |
| concepts[14].id | https://openalex.org/C6557445 |
| concepts[14].level | 1 |
| concepts[14].score | 0.13055062294006348 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q173113 |
| concepts[14].display_name | Agronomy |
| concepts[15].id | https://openalex.org/C54355233 |
| concepts[15].level | 1 |
| concepts[15].score | 0.09133702516555786 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[15].display_name | Genetics |
| keywords[0].id | https://openalex.org/keywords/recombinant-dna |
| keywords[0].score | 0.7675395011901855 |
| keywords[0].display_name | Recombinant DNA |
| keywords[1].id | https://openalex.org/keywords/clone |
| keywords[1].score | 0.5395334362983704 |
| keywords[1].display_name | clone (Java method) |
| keywords[2].id | https://openalex.org/keywords/green-fluorescent-protein |
| keywords[2].score | 0.5309215188026428 |
| keywords[2].display_name | Green fluorescent protein |
| keywords[3].id | https://openalex.org/keywords/fusion-protein |
| keywords[3].score | 0.5239855647087097 |
| keywords[3].display_name | Fusion protein |
| keywords[4].id | https://openalex.org/keywords/biotechnology |
| keywords[4].score | 0.48851120471954346 |
| keywords[4].display_name | Biotechnology |
| keywords[5].id | https://openalex.org/keywords/biomass |
| keywords[5].score | 0.48457157611846924 |
| keywords[5].display_name | Biomass (ecology) |
| keywords[6].id | https://openalex.org/keywords/food-science |
| keywords[6].score | 0.4612748622894287 |
| keywords[6].display_name | Food science |
| keywords[7].id | https://openalex.org/keywords/hydrophobin |
| keywords[7].score | 0.4381014406681061 |
| keywords[7].display_name | Hydrophobin |
| keywords[8].id | https://openalex.org/keywords/cell-culture |
| keywords[8].score | 0.43764230608940125 |
| keywords[8].display_name | Cell culture |
| keywords[9].id | https://openalex.org/keywords/biology |
| keywords[9].score | 0.4372156262397766 |
| keywords[9].display_name | Biology |
| keywords[10].id | https://openalex.org/keywords/transgene |
| keywords[10].score | 0.4205589294433594 |
| keywords[10].display_name | Transgene |
| keywords[11].id | https://openalex.org/keywords/chemistry |
| keywords[11].score | 0.34225931763648987 |
| keywords[11].display_name | Chemistry |
| keywords[12].id | https://openalex.org/keywords/biochemistry |
| keywords[12].score | 0.2981182932853699 |
| keywords[12].display_name | Biochemistry |
| keywords[13].id | https://openalex.org/keywords/gene |
| keywords[13].score | 0.1593596339225769 |
| keywords[13].display_name | Gene |
| keywords[14].id | https://openalex.org/keywords/agronomy |
| keywords[14].score | 0.13055062294006348 |
| keywords[14].display_name | Agronomy |
| keywords[15].id | https://openalex.org/keywords/genetics |
| keywords[15].score | 0.09133702516555786 |
| keywords[15].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.3389/fpls.2018.00045 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2493613807 |
| locations[0].source.issn | 1664-462X |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 1664-462X |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Frontiers in Plant Science |
| locations[0].source.host_organization | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_name | Frontiers Media |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_lineage_names | Frontiers Media |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Frontiers in Plant Science |
| locations[0].landing_page_url | https://doi.org/10.3389/fpls.2018.00045 |
| locations[1].id | pmid:29434617 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Frontiers in plant science |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/29434617 |
| locations[2].id | pmh:oai:doaj.org/article:c8cba93e82d74c379d1bd6740845304f |
| locations[2].is_oa | False |
| locations[2].source.id | https://openalex.org/S4306401280 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[2].source.host_organization | |
| locations[2].source.host_organization_name | |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Frontiers in Plant Science, Vol 9 (2018) |
| locations[2].landing_page_url | https://doaj.org/article/c8cba93e82d74c379d1bd6740845304f |
| locations[3].id | pmh:oai:europepmc.org:4715920 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400806 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | Europe PMC (PubMed Central) |
| locations[3].source.host_organization | https://openalex.org/I1303153112 |
| locations[3].source.host_organization_name | European Bioinformatics Institute |
| locations[3].source.host_organization_lineage | https://openalex.org/I1303153112 |
| locations[3].license | cc-by |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/cc-by |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | |
| locations[3].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/5791008 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5002260707 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-6959-3148 |
| authorships[0].author.display_name | Suvi T. Häkkinen |
| authorships[0].countries | FI |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[0].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[0].institutions[0].id | https://openalex.org/I87653560 |
| authorships[0].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[0].institutions[0].type | nonprofit |
| authorships[0].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[0].institutions[0].country_code | FI |
| authorships[0].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Suvi T. Häkkinen |
| authorships[0].is_corresponding | True |
| authorships[0].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[1].author.id | https://openalex.org/A5016602547 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-3350-7575 |
| authorships[1].author.display_name | Lauri Reuter |
| authorships[1].countries | FI |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[1].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[1].institutions[0].id | https://openalex.org/I87653560 |
| authorships[1].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[1].institutions[0].type | nonprofit |
| authorships[1].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[1].institutions[0].country_code | FI |
| authorships[1].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Lauri Reuter |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[2].author.id | https://openalex.org/A5060695140 |
| authorships[2].author.orcid | |
| authorships[2].author.display_name | Ninni Nuorti |
| authorships[2].countries | FI |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[2].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[2].institutions[0].id | https://openalex.org/I87653560 |
| authorships[2].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[2].institutions[0].type | nonprofit |
| authorships[2].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[2].institutions[0].country_code | FI |
| authorships[2].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Ninni Nuorti |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[3].author.id | https://openalex.org/A5109179084 |
| authorships[3].author.orcid | |
| authorships[3].author.display_name | Jussi J. Joensuu |
| authorships[3].countries | FI |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[3].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[3].institutions[0].id | https://openalex.org/I87653560 |
| authorships[3].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[3].institutions[0].type | nonprofit |
| authorships[3].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[3].institutions[0].country_code | FI |
| authorships[3].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Jussi J. Joensuu |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[4].author.id | https://openalex.org/A5048479269 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-8004-4526 |
| authorships[4].author.display_name | Heiko Rischer |
| authorships[4].countries | FI |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[4].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[4].institutions[0].id | https://openalex.org/I87653560 |
| authorships[4].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[4].institutions[0].type | nonprofit |
| authorships[4].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[4].institutions[0].country_code | FI |
| authorships[4].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Heiko Rischer |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[5].author.id | https://openalex.org/A5024503974 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-2406-6245 |
| authorships[5].author.display_name | Anneli Ritala |
| authorships[5].countries | FI |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I87653560 |
| authorships[5].affiliations[0].raw_affiliation_string | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| authorships[5].institutions[0].id | https://openalex.org/I87653560 |
| authorships[5].institutions[0].ror | https://ror.org/04b181w54 |
| authorships[5].institutions[0].type | nonprofit |
| authorships[5].institutions[0].lineage | https://openalex.org/I4210089493, https://openalex.org/I87653560 |
| authorships[5].institutions[0].country_code | FI |
| authorships[5].institutions[0].display_name | VTT Technical Research Centre of Finland |
| authorships[5].author_position | last |
| authorships[5].raw_author_name | Anneli Ritala |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | VTT Technical Research Centre of Finland Ltd., Espoo, Finland |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdf |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T12856 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 1.0 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1305 |
| primary_topic.subfield.display_name | Biotechnology |
| primary_topic.display_name | Transgenic Plants and Applications |
| related_works | https://openalex.org/W3108397060, https://openalex.org/W1422893055, https://openalex.org/W3015311203, https://openalex.org/W24093439, https://openalex.org/W2109840577, https://openalex.org/W2113540885, https://openalex.org/W2360861458, https://openalex.org/W2350892013, https://openalex.org/W2025879395, https://openalex.org/W2373087578 |
| cited_by_count | 46 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 6 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 7 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 8 |
| counts_by_year[3].year | 2022 |
| counts_by_year[3].cited_by_count | 9 |
| counts_by_year[4].year | 2021 |
| counts_by_year[4].cited_by_count | 7 |
| counts_by_year[5].year | 2020 |
| counts_by_year[5].cited_by_count | 5 |
| counts_by_year[6].year | 2019 |
| counts_by_year[6].cited_by_count | 2 |
| counts_by_year[7].year | 2018 |
| counts_by_year[7].cited_by_count | 2 |
| locations_count | 4 |
| best_oa_location.id | doi:10.3389/fpls.2018.00045 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2493613807 |
| best_oa_location.source.issn | 1664-462X |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 1664-462X |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Frontiers in Plant Science |
| best_oa_location.source.host_organization | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_name | Frontiers Media |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_lineage_names | Frontiers Media |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Frontiers in Plant Science |
| best_oa_location.landing_page_url | https://doi.org/10.3389/fpls.2018.00045 |
| primary_location.id | doi:10.3389/fpls.2018.00045 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2493613807 |
| primary_location.source.issn | 1664-462X |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 1664-462X |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Frontiers in Plant Science |
| primary_location.source.host_organization | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_name | Frontiers Media |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_lineage_names | Frontiers Media |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.frontiersin.org/articles/10.3389/fpls.2018.00045/pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Frontiers in Plant Science |
| primary_location.landing_page_url | https://doi.org/10.3389/fpls.2018.00045 |
| publication_date | 2018-01-26 |
| publication_year | 2018 |
| referenced_works | https://openalex.org/W4293247451, https://openalex.org/W6668394796, https://openalex.org/W2143139062, https://openalex.org/W2048898143, https://openalex.org/W2526544758, https://openalex.org/W2092198719, https://openalex.org/W2100866882, https://openalex.org/W2153080874, https://openalex.org/W2135916248, https://openalex.org/W2119370926, https://openalex.org/W1869955023, https://openalex.org/W2700920803, https://openalex.org/W2066998178, https://openalex.org/W2024556021, https://openalex.org/W1496077170, https://openalex.org/W1568903637, https://openalex.org/W2177976712, https://openalex.org/W2151614210, https://openalex.org/W1981027078, https://openalex.org/W2619029305, https://openalex.org/W2029629743, https://openalex.org/W2059222618, https://openalex.org/W2279824931, https://openalex.org/W2077038821, https://openalex.org/W572268322, https://openalex.org/W2128635872, https://openalex.org/W4244734200, https://openalex.org/W2581793735, https://openalex.org/W2069974821 |
| referenced_works_count | 29 |
| abstract_inverted_index.I | 81 |
| abstract_inverted_index.In | 42, 117 |
| abstract_inverted_index.an | 3 |
| abstract_inverted_index.as | 11, 29 |
| abstract_inverted_index.in | 25, 56, 107 |
| abstract_inverted_index.of | 8, 22, 52, 64, 128 |
| abstract_inverted_index.to | 48, 87, 143, 147, 152 |
| abstract_inverted_index.up | 146 |
| abstract_inverted_index.± | 103 |
| abstract_inverted_index.0.2 | 104 |
| abstract_inverted_index.1.1 | 102 |
| abstract_inverted_index.69% | 148 |
| abstract_inverted_index.One | 21, 94 |
| abstract_inverted_index.aim | 46 |
| abstract_inverted_index.and | 13, 17, 66, 73, 109, 145 |
| abstract_inverted_index.are | 19 |
| abstract_inverted_index.for | 6 |
| abstract_inverted_index.g/l | 105 |
| abstract_inverted_index.has | 32 |
| abstract_inverted_index.its | 111 |
| abstract_inverted_index.the | 23, 34, 45, 50, 57, 62, 89, 134 |
| abstract_inverted_index.was | 47, 156 |
| abstract_inverted_index.BY-2 | 71, 76, 97 |
| abstract_inverted_index.With | 133 |
| abstract_inverted_index.been | 33 |
| abstract_inverted_index.both | 119 |
| abstract_inverted_index.cell | 131 |
| abstract_inverted_index.cost | 139 |
| abstract_inverted_index.from | 37 |
| abstract_inverted_index.high | 112 |
| abstract_inverted_index.kept | 110 |
| abstract_inverted_index.more | 12, 14 |
| abstract_inverted_index.most | 53, 90 |
| abstract_inverted_index.this | 43, 129 |
| abstract_inverted_index.used | 86 |
| abstract_inverted_index.were | 85, 121 |
| abstract_inverted_index.with | 141, 150 |
| abstract_inverted_index.Plant | 0 |
| abstract_inverted_index.cells | 1, 28 |
| abstract_inverted_index.costs | 35 |
| abstract_inverted_index.green | 78 |
| abstract_inverted_index.host. | 132 |
| abstract_inverted_index.hosts | 31 |
| abstract_inverted_index.named | 99 |
| abstract_inverted_index.plant | 27, 130 |
| abstract_inverted_index.truly | 125 |
| abstract_inverted_index.using | 26 |
| abstract_inverted_index.work, | 44 |
| abstract_inverted_index.43-55% | 138 |
| abstract_inverted_index.clone, | 98 |
| abstract_inverted_index.during | 114 |
| abstract_inverted_index.fusion | 83 |
| abstract_inverted_index.levels | 51 |
| abstract_inverted_index.medium | 40, 59, 92 |
| abstract_inverted_index.regard | 142, 151 |
| abstract_inverted_index.'Hulk,' | 100 |
| abstract_inverted_index.biomass | 65, 144 |
| abstract_inverted_index.culture | 39, 72, 136 |
| abstract_inverted_index.medium, | 137 |
| abstract_inverted_index.protein | 68, 84, 154 |
| abstract_inverted_index.tobacco | 75 |
| abstract_inverted_index.without | 60 |
| abstract_inverted_index.yields. | 69 |
| abstract_inverted_index.GFP-HFBI | 106 |
| abstract_inverted_index.cultures | 120 |
| abstract_inverted_index.deriving | 36 |
| abstract_inverted_index.desired. | 20 |
| abstract_inverted_index.enabling | 124 |
| abstract_inverted_index.nutrient | 58 |
| abstract_inverted_index.optimize | 49 |
| abstract_inverted_index.platform | 5 |
| abstract_inverted_index.produced | 101 |
| abstract_inverted_index.products | 16 |
| abstract_inverted_index.proteins | 10 |
| abstract_inverted_index.Wild-type | 70 |
| abstract_inverted_index.achieved. | 157 |
| abstract_inverted_index.addition, | 118 |
| abstract_inverted_index.determine | 88 |
| abstract_inverted_index.expensive | 38, 54 |
| abstract_inverted_index.optimized | 135 |
| abstract_inverted_index.processes | 18 |
| abstract_inverted_index.prolonged | 115 |
| abstract_inverted_index.reduction | 140, 149 |
| abstract_inverted_index.(GFP-HFBI) | 82 |
| abstract_inverted_index.attractive | 4 |
| abstract_inverted_index.challenges | 24 |
| abstract_inverted_index.components | 55 |
| abstract_inverted_index.constitute | 2 |
| abstract_inverted_index.expressing | 77 |
| abstract_inverted_index.industrial | 126 |
| abstract_inverted_index.production | 7, 30, 155 |
| abstract_inverted_index.suspension | 108 |
| abstract_inverted_index.transgenic | 74 |
| abstract_inverted_index.animal-free | 15 |
| abstract_inverted_index.application | 127 |
| abstract_inverted_index.components. | 41 |
| abstract_inverted_index.fluorescent | 79 |
| abstract_inverted_index.inexpensive | 91 |
| abstract_inverted_index.performance | 113 |
| abstract_inverted_index.recombinant | 9, 67, 153 |
| abstract_inverted_index.accumulation | 63 |
| abstract_inverted_index.composition. | 93 |
| abstract_inverted_index.compromising | 61 |
| abstract_inverted_index.particularly | 95 |
| abstract_inverted_index.successfully | 122 |
| abstract_inverted_index.cryopreserved | 123 |
| abstract_inverted_index.subculturing. | 116 |
| abstract_inverted_index.high-accumulating | 96 |
| abstract_inverted_index.protein-Hydrophobin | 80 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 94 |
| corresponding_author_ids | https://openalex.org/A5002260707 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 6 |
| corresponding_institution_ids | https://openalex.org/I87653560 |
| citation_normalized_percentile.value | 0.90280533 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |