Tomographic-encoded multiphoton (TEMP) microscopy Article Swipe
Hongsen He
,
Xinyong Dong
,
Yu-xuan Ren
,
Cora Sau Wan Lai
,
Kevin K. Tsia
,
Kenneth K. Wong
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.04.11.487875
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1101/2022.04.11.487875
Axial scanning in multiphoton microscopy (MPM) is typically realized by mechanically shifting either the objective or the sample. However, the scan speed is usually hindered by the mechanical inertia of the bulky mass. Although the extended depth of field provided by the non-diffracting beam allows fast volumetric imaging, it abandons the axial resolution. Here, we demonstrate a novel and powerful tomographic technique using the Bessel droplet in MPM, termed Tomographic-Encoded MultiPhoton (TEMP) microscopy. We show that benefiting from the high-order nonlinear excitation in MPM, the side-lobes cancellation and smaller beam focus of the Bessel droplet realize better image quality. The TEMP microscopy allows fast axial scanning, less risks of photodamage and photobleaching, and high-resolution and high-contrast imaging. Furthermore, fewer raw images are required for the 3D image reconstruction. To demonstrate its usability and advantages for scattering tissues and biomedical applications, we showcase the TEMP microscopy with highly scattering fluorescence microspheres and mouse brain slice. More details can be visualized by the Bessel droplet compared with the conventional Gaussian and Bessel beam. More importantly, the TEMP technique is an easy-plug-in method for the current microscopy system. The TEMP microscopy is promising for fast volumetric multiphoton imaging, especially for highly scattering tissues.
Related Topics
Concepts
Bessel beam
Microscopy
Optics
Light sheet fluorescence microscopy
Materials science
Tomographic reconstruction
Photobleaching
Scattering
Tomography
Beam (structure)
Physics
Scanning confocal electron microscopy
Fluorescence
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2022.04.11.487875
- https://www.biorxiv.org/content/biorxiv/early/2022/04/11/2022.04.11.487875.full.pdf
- OA Status
- green
- References
- 38
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4223541110
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4223541110Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2022.04.11.487875Digital Object Identifier
- Title
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Tomographic-encoded multiphoton (TEMP) microscopyWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-04-11Full publication date if available
- Authors
-
Hongsen He, Xinyong Dong, Yu-xuan Ren, Cora Sau Wan Lai, Kevin K. Tsia, Kenneth K. WongList of authors in order
- Landing page
-
https://doi.org/10.1101/2022.04.11.487875Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2022/04/11/2022.04.11.487875.full.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2022/04/11/2022.04.11.487875.full.pdfDirect OA link when available
- Concepts
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Bessel beam, Microscopy, Optics, Light sheet fluorescence microscopy, Materials science, Tomographic reconstruction, Photobleaching, Scattering, Tomography, Beam (structure), Physics, Scanning confocal electron microscopy, FluorescenceTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- References (count)
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38Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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