Transcriptome based weighted gene coexpression network analysis reveals synergistic effects of darkness and mechanical stimulation on pod development in peanut (Arachis hypogea L.) Article Swipe
YOU?
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· 2024
· Open Access
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· DOI: https://doi.org/10.21203/rs.3.rs-5400642/v1
· OA: W4405081516
<title>Abstract</title> <bold>Background</bold>: Peanut is an important oil crop with atypical fruitification pattern which require darkness and mechanical stimulation to facilitate normal pod development. Despite some progress in understanding peanut pod development and its response to external environmental stimulation, numerous unresolved questions and knowledge gaps remain regarding the role of darkness and mechanical stimulation in this complex process. <bold>Results</bold>: In this study, we managed to investigate the impacts of dark and mechanical stimulation on peanut pod development via transcriptome. A total of 55,087 genes along with a series of DEGs and pathways were identified among different treatment groups (CK, TB, TML and TMB) that play crucial roles, and offers a novel perspective on the role of photosynthesis during peanut pod development. Moreover, by utilizing weighted gene coexpression network analysis (WGCNA) we identified several hub genes (e.g., <italic>IAA9</italic> (<italic>Ahy_B07g086610</italic>), <italic>BSK5</italic> (<italic>Ahy_B03g068305</italic>), <italic>GRF7</italic> (<italic>Ahy_B10g103808</italic>), and <italic>PER17</italic> (<italic>Ahy_B10g105104</italic>)) and key pathways (e.g., plant hormonal and signal transduction pathway, and lignin biosynthesis pathway) that might be true candidates for peanut pod development. Further, the expression patterns of key candidates were validated via qRT-PCR during different pod development stages. <bold>Conclusions</bold>: Overall, our findings provide valuable insights into the mechanisms underlying peanut pod development in responding to darkness and mechanical stimulations.
