Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation Article Swipe

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Xuyang Wang , Ran Cheng , Chenyang Wu , Haiyin Liu , Zining Li , Yunfei Huo , Bo Li , Dongyu Zhao , Li Cheng , Hongkui Deng , Jun Xu ·

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.1093/procel/pwaf059

Mouse extended pluripotent stem (EPS) cells have demonstrated significant potential for generating embryo models in vitro. However, their limited capacity for extraembryonic trophoblast development has hindered their use in constructing whole embryo models, particularly post-implantation embryoids. Here, we establish a stepwise induction protocol to generate trophectoderm-like cells from mouse EPS cells. These cells retain trophectoderm-specific transcriptomic features and can differentiate into trophoblast lineages in vivo. Moreover, combining these trophectoderm-like cells with EPS cell-derived primitive endoderm/epiblast bilineage structures enabled the robust generation of post-implantation embryoids in a transgene-free manner. EPS-derived embryoids recapitulate key developmental events of post-implantation mouse embryos, including the formation of the pro-amniotic cavity, anterior-posterior axis, primitive streak, gastrulation, and complex extraembryonic tissues. Notably, single-cell transcriptomic analysis revealed a high degree of transcriptional similarity between EPS-derived embryoids at day 6 and natural E7.5 mouse embryos. Our study presents a novel platform for modeling post-implantation mouse embryogenesis in vitro.

Related Topics

Gastrulation

Cell Biology

Embryo

Developmental Biology

Concepts

Gastrulation Cell biology Embryo Developmental biology Biology Stem cell Blastocyst Cell Embryogenesis Genetics

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Type: article
Language: en
Landing Page: https://doi.org/10.1093/procel/pwaf059
OA Status: diamond
References: 94
Related Works: 10
OpenAlex ID: https://openalex.org/W4413115292

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DOI: https://doi.org/10.1093/procel/pwaf059

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Title: Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation

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Type: article

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Language: en

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Publication year: 2025

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Publication date: 2025-08-11

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Authors: Xuyang Wang, Ran Cheng, Chenyang Wu, Haiyin Liu, Zining Li, Yunfei Huo, Bo Li, Dongyu Zhao, Li Cheng, Hongkui Deng, Jun Xu

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Landing page: https://doi.org/10.1093/procel/pwaf059

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Concepts: Gastrulation, Cell biology, Embryo, Developmental biology, Biology, Stem cell, Blastocyst, Cell, Embryogenesis, Genetics

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References (count): 94

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