Tumor cell lines expressing memHsp70 are able to bind TPP at 4°C. Article Swipe

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Chemistry Cell culture Cell biology Molecular biology Computational biology Biology Genetics

Gehrmann Mathias , Stangl Stefan , Andrea Gemma , Rupert Oellinger , Stephanie Breuninger , Rad Roland , G. Pockley Alan , Multhoff Gabriele ·

YOU? · · 2015 · Open Access · · DOI: https://doi.org/10.1371/journal.pone.0105344.g001 · OA: W2247245583

<p>The memHsp70 status of a panel of human breast, colon, melanoma, lung, head & neck tumor cell lines was assessed using the cmHsp70.1 antibody (top row histograms). Incubation of tumor cells with carboxyfluorescein (CF)-labeled TPP at 4°C (bottom row histograms) results in a similar binding profile to that of cmHsp70.1 antibody in all tumor cell lines; grey, isotype controls, open histograms, Hsp70 specific reagents. The numbers in the histograms show the proportion of Hsp70 membrane-positively stained cells.</p>