TWINKLE: An open-source two-photon microscope for teaching and research Article Swipe
Manuel Schottdorf
,
Phil Rich
,
E. Mika Diamanti
,
Albert Lin
,
Sina Tafazoli
,
Edward H. Nieh
,
Stephan Y. Thiberge
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0318924
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0318924
Many laboratories use two-photon microscopy through commercial suppliers, or homemade designs of considerable complexity. The integrated nature of these systems complicates customization, troubleshooting, and training on the principles of two-photon microscopy. Here, we present “Twinkle”: a microscope for Two-photon Imaging in Neuroscience, and Kit for Learning and Education. It is a fully open, high performing and easy-to-set-up microscope that can effectively be used for both education and research. The instrument features a >1 mm field of view, using a modern objective with 3 mm working distance and 2 inch diameter optics combined with GaAsP photomultiplier tubes to maximize the fluorescence signal. We document our experiences using this system as a teaching tool in several two week long workshops, exemplify scientific use cases, and conclude with a broader note on the place of our work in the growing space of open scientific instrumentation.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0318924
- OA Status
- gold
- References
- 93
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4407461659
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4407461659Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1371/journal.pone.0318924Digital Object Identifier
- Title
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TWINKLE: An open-source two-photon microscope for teaching and researchWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-02-13Full publication date if available
- Authors
-
Manuel Schottdorf, Phil Rich, E. Mika Diamanti, Albert Lin, Sina Tafazoli, Edward H. Nieh, Stephan Y. ThibergeList of authors in order
- Landing page
-
https://doi.org/10.1371/journal.pone.0318924Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1371/journal.pone.0318924Direct OA link when available
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Microscope, Troubleshooting, Photomultiplier, Instrumentation (computer programming), Computer science, Personalization, Optics, Two-photon excitation microscopy, Photon, Nanotechnology, Physics, Materials science, Fluorescence, World Wide Web, Operating system, DetectorTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- References (count)
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93Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.complexity. | 13 |
| abstract_inverted_index.complicates | 20 |
| abstract_inverted_index.effectively | 60 |
| abstract_inverted_index.experiences | 104 |
| abstract_inverted_index.microscopy. | 30 |
| abstract_inverted_index.considerable | 12 |
| abstract_inverted_index.fluorescence | 99 |
| abstract_inverted_index.laboratories | 1 |
| abstract_inverted_index.Neuroscience, | 41 |
| abstract_inverted_index.customization, | 21 |
| abstract_inverted_index.easy-to-set-up | 56 |
| abstract_inverted_index.“Twinkle”: | 34 |
| abstract_inverted_index.photomultiplier | 94 |
| abstract_inverted_index.instrumentation. | 141 |
| abstract_inverted_index.troubleshooting, | 22 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5031770042, https://openalex.org/A5006975038, https://openalex.org/A5041459537, https://openalex.org/A5090075554, https://openalex.org/A5037104024, https://openalex.org/A5045704652, https://openalex.org/A5067274774 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 7 |
| corresponding_institution_ids | https://openalex.org/I20089843, https://openalex.org/I51556381, https://openalex.org/I86501945 |
| citation_normalized_percentile.value | 0.02721316 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |