Unravelling Form and Function: Improved function of engineered cardiac tissue through extra-cellular anisotropy Article Swipe
Jamie A. Cyr
,
Maria Colzani
,
Semih Bayraktar
,
Vera Graup
,
Richard W. Farndale
,
Sanjay Sinha
,
Serena M. Best
,
Ruth E. Cameron
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.08.06.455438
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.08.06.455438
Cardiac tissue engineering is a promising therapeutic option for myocardial repair after injury, however, so far engineered heart patches have shown limited translational utility due to poor electrical integration and tissue contractility. Emerging research suggests that scaffolds that recapitulate the three-dimensional structure of the native myocardium improve physiological function. Complex scaffold fabrication remains a technical challenge and the isolated impact of scaffold architecture on tissue function and cellular physiology is poorly understood. Here, we provided a direct comparison between isotropic and aligned collagen scaffold morphologies where all confounding physio-mechanical features, such as strut wall thickness and surface roughness are conserved. This enabled the independent and systematic assessment of the effects of pore macro-architecture on global tissue function and cellular maturation. We seeded our scaffolds with embryonic stem cell derived cardiomyocytes (hESC-CM) and measured tissue function through calcium signal transduction and dynamic contractile strain. The aligned tissue constructs facilitated improved signalling synchronicity and directional contractility. We further examined the influence of scaffold macrostructure on intercellular organization and intracellular development. Cells on aligned constructs conformed to the orientation of the scaffold macro-structure and were found to have phenotypic and genetic markers of increased maturity. Our results isolate the influence of scaffold macro-structure on engineered tissue function at multiple length scales. These findings inform the design of optimized cardiac tissue and expand the potential for engineered tissue in regenerative and model medical systems by reducing the gaps in tissue functionality that limit their utility.
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- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2021.08.06.455438
- https://www.biorxiv.org/content/biorxiv/early/2021/08/08/2021.08.06.455438.full.pdf
- OA Status
- green
- Cited By
- 1
- References
- 75
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3192419161
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3192419161Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2021.08.06.455438Digital Object Identifier
- Title
-
Unravelling Form and Function: Improved function of engineered cardiac tissue through extra-cellular anisotropyWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-08-08Full publication date if available
- Authors
-
Jamie A. Cyr, Maria Colzani, Semih Bayraktar, Vera Graup, Richard W. Farndale, Sanjay Sinha, Serena M. Best, Ruth E. CameronList of authors in order
- Landing page
-
https://doi.org/10.1101/2021.08.06.455438Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/08/08/2021.08.06.455438.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/08/08/2021.08.06.455438.full.pdfDirect OA link when available
- Concepts
-
Scaffold, Tissue engineering, Cell biology, Contractility, Biomedical engineering, Embryonic stem cell, Paracrine signalling, Regenerative medicine, Chemistry, Biology, Stem cell, Medicine, Biochemistry, Endocrinology, Receptor, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
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2023: 1Per-year citation counts (last 5 years)
- References (count)
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75Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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