USING A CACO-2 AND THP-1 CO-CULTURE MODEL TO EVALUATE INTESTINAL BARRIER PROTECTIVE PROPERTIES — EFFECTS OF DEXAMETHASONE Article Swipe

PDF

Sylvia Riedel , Lize Engelbrecht , Pieter Venter , Johan Louw , Carmen Pheiffer , Christo J. F. Muller ·

YOU? · · 2022 · Open Access · · DOI: https://doi.org/10.15605/jafes.037.afes.04

OBJECTIVES Increased intestinal permeability or so-called “leaky gut,” is considered an early event in the development of obesity and diabetes. It is likely driven by the translocation of bacteria-derived products such as lipopolysaccharide (LPS) from the intestinal lumen into the blood stream leading to low-grade inflammation. A co-culture model of intestinal barrier dysfunction was used to characterize the mechanisms of barrier protection induced by an anti-inflammatory drug METHODOLOGY In co-cultured CACO-2 and THP-1 cells representing the intestinal epithelium and immune cells, respectively, barrier dysfunction was induced using LPS and quantified by measuring trans-epithelial electrical resistance (TEER). Dexamethasone was tested for its barrier-protective properties by application to the apical side. Dexamethasone concentration was quantified on apical and basolateral sides after 24-hour treatment using liquid chromatography/mass spectrometry. Cytokine release was determined using a multiplex chemiluminescence assay (mesoscale discovery). Expression of selected tight junction (TJ) proteins was assessed using immunocytochemical staining and confocal imaging. RESULTS Dexamethasone significantly (p=0.009) improved TEER by 30%, while 74% of the dexamethasone remained on the apical side and 24% was detected basolaterally. Dexamethasone significantly decreased interleukin (IL)-6 (p<0.0001), tumour necrosis factor α (p<0.0001) and IL-1β (p=0.051) release by THP-1 cells. However, confocal imaging revealed that dexamethasone did not improve the localization of occludin, claudin-2 or zonula occludens-1 when compared to LPS-induced cells. CONCLUSION Dexamethasone improved barrier function likely due to its anti-inflammatory effect. However, it could not prevent localization of the pore-forming claudin-2, which may explain the presence of residual barrier dysfunction in this model.

Related Topics

Concepts

Medicine Dexamethasone THP1 cell line Caco-2 Internal medicine In vitro Cell culture Biochemistry Chemistry Genetics Biology

Metadata

Type: article
Language: en
Landing Page: https://doi.org/10.15605/jafes.037.afes.04
PDF: https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959
OA Status: diamond
Related Works: 10
OpenAlex ID: https://openalex.org/W4412010328

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4412010328

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.15605/jafes.037.afes.04

Digital Object Identifier
Title: USING A CACO-2 AND THP-1 CO-CULTURE MODEL TO EVALUATE INTESTINAL BARRIER PROTECTIVE PROPERTIES — EFFECTS OF DEXAMETHASONE

Work title
Type: article

OpenAlex work type
Language: en

Primary language
Publication year: 2022

Year of publication
Publication date: 2022-10-14

Full publication date if available
Authors: Sylvia Riedel, Lize Engelbrecht, Pieter Venter, Johan Louw, Carmen Pheiffer, Christo J. F. Muller

List of authors in order
Landing page: https://doi.org/10.15605/jafes.037.afes.04

Publisher landing page
PDF URL: https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959

Direct link to full text PDF
Open access: Yes

Whether a free full text is available
OA status: diamond

Open access status per OpenAlex
OA URL: https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959

Direct OA link when available
Concepts: Medicine, Dexamethasone, THP1 cell line, Caco-2, Internal medicine, In vitro, Cell culture, Biochemistry, Chemistry, Genetics, Biology

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4412010328
doi	https://doi.org/10.15605/jafes.037.afes.04
ids.doi	https://doi.org/10.15605/jafes.037.afes.04
ids.openalex	https://openalex.org/W4412010328
fwci	0.0
type	article
title	USING A CACO-2 AND THP-1 CO-CULTURE MODEL TO EVALUATE INTESTINAL BARRIER PROTECTIVE PROPERTIES — EFFECTS OF DEXAMETHASONE
biblio.issue	2
biblio.volume	37
biblio.last_page	4
biblio.first_page	4
topics[0].id	https://openalex.org/T12626
topics[0].field.id	https://openalex.org/fields/27
topics[0].field.display_name	Medicine
topics[0].score	0.9854999780654907
topics[0].domain.id	https://openalex.org/domains/4
topics[0].domain.display_name	Health Sciences
topics[0].subfield.id	https://openalex.org/subfields/2738
topics[0].subfield.display_name	Psychiatry and Mental health
topics[0].display_name	Cancer, Stress, Anesthesia, and Immune Response
topics[1].id	https://openalex.org/T10276
topics[1].field.id	https://openalex.org/fields/27
topics[1].field.display_name	Medicine
topics[1].score	0.9789000153541565
topics[1].domain.id	https://openalex.org/domains/4
topics[1].domain.display_name	Health Sciences
topics[1].subfield.id	https://openalex.org/subfields/2746
topics[1].subfield.display_name	Surgery
topics[1].display_name	Helicobacter pylori-related gastroenterology studies
topics[2].id	https://openalex.org/T10371
topics[2].field.id	https://openalex.org/fields/24
topics[2].field.display_name	Immunology and Microbiology
topics[2].score	0.9466000199317932
topics[2].domain.id	https://openalex.org/domains/1
topics[2].domain.display_name	Life Sciences
topics[2].subfield.id	https://openalex.org/subfields/2403
topics[2].subfield.display_name	Immunology
topics[2].display_name	Immune Response and Inflammation
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C71924100
concepts[0].level	0
concepts[0].score	0.8704208731651306
concepts[0].wikidata	https://www.wikidata.org/wiki/Q11190
concepts[0].display_name	Medicine
concepts[1].id	https://openalex.org/C2780401358
concepts[1].level	2
concepts[1].score	0.6537505984306335
concepts[1].wikidata	https://www.wikidata.org/wiki/Q422252
concepts[1].display_name	Dexamethasone
concepts[2].id	https://openalex.org/C204258353
concepts[2].level	3
concepts[2].score	0.6277040839195251
concepts[2].wikidata	https://www.wikidata.org/wiki/Q7670282
concepts[2].display_name	THP1 cell line
concepts[3].id	https://openalex.org/C193176779
concepts[3].level	3
concepts[3].score	0.5253010392189026
concepts[3].wikidata	https://www.wikidata.org/wiki/Q5016050
concepts[3].display_name	Caco-2
concepts[4].id	https://openalex.org/C126322002
concepts[4].level	1
concepts[4].score	0.2900071144104004
concepts[4].wikidata	https://www.wikidata.org/wiki/Q11180
concepts[4].display_name	Internal medicine
concepts[5].id	https://openalex.org/C202751555
concepts[5].level	2
concepts[5].score	0.16550037264823914
concepts[5].wikidata	https://www.wikidata.org/wiki/Q221681
concepts[5].display_name	In vitro
concepts[6].id	https://openalex.org/C81885089
concepts[6].level	2
concepts[6].score	0.14222130179405212
concepts[6].wikidata	https://www.wikidata.org/wiki/Q189082
concepts[6].display_name	Cell culture
concepts[7].id	https://openalex.org/C55493867
concepts[7].level	1
concepts[7].score	0.10399022698402405
concepts[7].wikidata	https://www.wikidata.org/wiki/Q7094
concepts[7].display_name	Biochemistry
concepts[8].id	https://openalex.org/C185592680
concepts[8].level	0
concepts[8].score	0.0
concepts[8].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[8].display_name	Chemistry
concepts[9].id	https://openalex.org/C54355233
concepts[9].level	1
concepts[9].score	0.0
concepts[9].wikidata	https://www.wikidata.org/wiki/Q7162
concepts[9].display_name	Genetics
concepts[10].id	https://openalex.org/C86803240
concepts[10].level	0
concepts[10].score	0.0
concepts[10].wikidata	https://www.wikidata.org/wiki/Q420
concepts[10].display_name	Biology
keywords[0].id	https://openalex.org/keywords/medicine
keywords[0].score	0.8704208731651306
keywords[0].display_name	Medicine
keywords[1].id	https://openalex.org/keywords/dexamethasone
keywords[1].score	0.6537505984306335
keywords[1].display_name	Dexamethasone
keywords[2].id	https://openalex.org/keywords/thp1-cell-line
keywords[2].score	0.6277040839195251
keywords[2].display_name	THP1 cell line
keywords[3].id	https://openalex.org/keywords/caco-2
keywords[3].score	0.5253010392189026
keywords[3].display_name	Caco-2
keywords[4].id	https://openalex.org/keywords/internal-medicine
keywords[4].score	0.2900071144104004
keywords[4].display_name	Internal medicine
keywords[5].id	https://openalex.org/keywords/in-vitro
keywords[5].score	0.16550037264823914
keywords[5].display_name	In vitro
keywords[6].id	https://openalex.org/keywords/cell-culture
keywords[6].score	0.14222130179405212
keywords[6].display_name	Cell culture
keywords[7].id	https://openalex.org/keywords/biochemistry
keywords[7].score	0.10399022698402405
keywords[7].display_name	Biochemistry
language	en
locations[0].id	doi:10.15605/jafes.037.afes.04
locations[0].is_oa	True
locations[0].source.id	https://openalex.org/S2737532273
locations[0].source.issn	0857-1074, 2308-118X
locations[0].source.type	journal
locations[0].source.is_oa	True
locations[0].source.issn_l	0857-1074
locations[0].source.is_core	True
locations[0].source.is_in_doaj	True
locations[0].source.display_name	Journal of the ASEAN Federation of Endocrine Societies
locations[0].source.host_organization	https://openalex.org/P4310319565
locations[0].source.host_organization_name	ASEAN Federation of Endocrine Societies
locations[0].source.host_organization_lineage	https://openalex.org/P4310319565
locations[0].source.host_organization_lineage_names	ASEAN Federation of Endocrine Societies
locations[0].license	cc-by-nc
locations[0].pdf_url	https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959
locations[0].version	publishedVersion
locations[0].raw_type	journal-article
locations[0].license_id	https://openalex.org/licenses/cc-by-nc
locations[0].is_accepted	True
locations[0].is_published	True
locations[0].raw_source_name	Journal of the ASEAN Federation of Endocrine Societies
locations[0].landing_page_url	https://doi.org/10.15605/jafes.037.afes.04
indexed_in	crossref, doaj
authorships[0].author.id	https://openalex.org/A5003427468
authorships[0].author.orcid	https://orcid.org/0000-0002-3439-9238
authorships[0].author.display_name	Sylvia Riedel
authorships[0].author_position	first
authorships[0].raw_author_name	Sylvia Riedel
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5118813198
authorships[1].author.orcid
authorships[1].author.display_name	Lize Engelbrecht
authorships[1].author_position	middle
authorships[1].raw_author_name	Lize Engelbrecht
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5018128566
authorships[2].author.orcid	https://orcid.org/0000-0003-3871-8432
authorships[2].author.display_name	Pieter Venter
authorships[2].author_position	middle
authorships[2].raw_author_name	Pieter Venter
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5014988741
authorships[3].author.orcid	https://orcid.org/0000-0002-9023-1659
authorships[3].author.display_name	Johan Louw
authorships[3].author_position	middle
authorships[3].raw_author_name	Johan Louw
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5075451018
authorships[4].author.orcid	https://orcid.org/0000-0002-0707-1552
authorships[4].author.display_name	Carmen Pheiffer
authorships[4].author_position	middle
authorships[4].raw_author_name	Carmen Pheiffer
authorships[4].is_corresponding	False
authorships[5].author.id	https://openalex.org/A5006611112
authorships[5].author.orcid	https://orcid.org/0000-0001-6821-2120
authorships[5].author.display_name	Christo J. F. Muller
authorships[5].author_position	last
authorships[5].raw_author_name	Christo J.F. Muller
authorships[5].is_corresponding	False
has_content.pdf	True
has_content.grobid_xml	True
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959
open_access.oa_status	diamond
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	USING A CACO-2 AND THP-1 CO-CULTURE MODEL TO EVALUATE INTESTINAL BARRIER PROTECTIVE PROPERTIES — EFFECTS OF DEXAMETHASONE
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T12626
primary_topic.field.id	https://openalex.org/fields/27
primary_topic.field.display_name	Medicine
primary_topic.score	0.9854999780654907
primary_topic.domain.id	https://openalex.org/domains/4
primary_topic.domain.display_name	Health Sciences
primary_topic.subfield.id	https://openalex.org/subfields/2738
primary_topic.subfield.display_name	Psychiatry and Mental health
primary_topic.display_name	Cancer, Stress, Anesthesia, and Immune Response
related_works	https://openalex.org/W2899084033, https://openalex.org/W2748952813, https://openalex.org/W3031052312, https://openalex.org/W4389568370, https://openalex.org/W3032375762, https://openalex.org/W1995515455, https://openalex.org/W2080531066, https://openalex.org/W3108674512, https://openalex.org/W1506200166, https://openalex.org/W1489783725
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.15605/jafes.037.afes.04
best_oa_location.is_oa	True
best_oa_location.source.id	https://openalex.org/S2737532273
best_oa_location.source.issn	0857-1074, 2308-118X
best_oa_location.source.type	journal
best_oa_location.source.is_oa	True
best_oa_location.source.issn_l	0857-1074
best_oa_location.source.is_core	True
best_oa_location.source.is_in_doaj	True
best_oa_location.source.display_name	Journal of the ASEAN Federation of Endocrine Societies
best_oa_location.source.host_organization	https://openalex.org/P4310319565
best_oa_location.source.host_organization_name	ASEAN Federation of Endocrine Societies
best_oa_location.source.host_organization_lineage	https://openalex.org/P4310319565
best_oa_location.source.host_organization_lineage_names	ASEAN Federation of Endocrine Societies
best_oa_location.license	cc-by-nc
best_oa_location.pdf_url	https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959
best_oa_location.version	publishedVersion
best_oa_location.raw_type	journal-article
best_oa_location.license_id	https://openalex.org/licenses/cc-by-nc
best_oa_location.is_accepted	True
best_oa_location.is_published	True
best_oa_location.raw_source_name	Journal of the ASEAN Federation of Endocrine Societies
best_oa_location.landing_page_url	https://doi.org/10.15605/jafes.037.afes.04
primary_location.id	doi:10.15605/jafes.037.afes.04
primary_location.is_oa	True
primary_location.source.id	https://openalex.org/S2737532273
primary_location.source.issn	0857-1074, 2308-118X
primary_location.source.type	journal
primary_location.source.is_oa	True
primary_location.source.issn_l	0857-1074
primary_location.source.is_core	True
primary_location.source.is_in_doaj	True
primary_location.source.display_name	Journal of the ASEAN Federation of Endocrine Societies
primary_location.source.host_organization	https://openalex.org/P4310319565
primary_location.source.host_organization_name	ASEAN Federation of Endocrine Societies
primary_location.source.host_organization_lineage	https://openalex.org/P4310319565
primary_location.source.host_organization_lineage_names	ASEAN Federation of Endocrine Societies
primary_location.license	cc-by-nc
primary_location.pdf_url	https://asean-endocrinejournal.org/index.php/JAFES/article/download/5827/4959
primary_location.version	publishedVersion
primary_location.raw_type	journal-article
primary_location.license_id	https://openalex.org/licenses/cc-by-nc
primary_location.is_accepted	True
primary_location.is_published	True
primary_location.raw_source_name	Journal of the ASEAN Federation of Endocrine Societies
primary_location.landing_page_url	https://doi.org/10.15605/jafes.037.afes.04
publication_date	2022-10-14
publication_year	2022
referenced_works_count	0
abstract_inverted_index.A	46
abstract_inverted_index.a	130
abstract_inverted_index.In	68
abstract_inverted_index.It	20
abstract_inverted_index.an	10, 64
abstract_inverted_index.as	31
abstract_inverted_index.by	24, 63, 90, 103, 157, 189
abstract_inverted_index.in	13, 244
abstract_inverted_index.is	8, 21
abstract_inverted_index.it	226
abstract_inverted_index.of	16, 27, 49, 59, 137, 161, 203, 231, 240
abstract_inverted_index.on	113, 165
abstract_inverted_index.or	4, 206
abstract_inverted_index.to	43, 55, 105, 211, 221
abstract_inverted_index.α	183
abstract_inverted_index.24%	170
abstract_inverted_index.74%	160
abstract_inverted_index.LPS	87
abstract_inverted_index.and	18, 71, 78, 88, 115, 148, 169, 185
abstract_inverted_index.did	198
abstract_inverted_index.due	220
abstract_inverted_index.for	99
abstract_inverted_index.its	100, 222
abstract_inverted_index.may	236
abstract_inverted_index.not	199, 228
abstract_inverted_index.the	14, 25, 35, 39, 57, 75, 106, 162, 166, 201, 232, 238
abstract_inverted_index.was	53, 84, 97, 111, 127, 143, 171
abstract_inverted_index.(TJ)	141
abstract_inverted_index.30%,	158
abstract_inverted_index.TEER	156
abstract_inverted_index.drug	66
abstract_inverted_index.from	34
abstract_inverted_index.into	38
abstract_inverted_index.side	168
abstract_inverted_index.such	30
abstract_inverted_index.that	196
abstract_inverted_index.this	245
abstract_inverted_index.used	54
abstract_inverted_index.when	209
abstract_inverted_index.(LPS)	33
abstract_inverted_index.THP-1	72, 190
abstract_inverted_index.after	118
abstract_inverted_index.assay	133
abstract_inverted_index.blood	40
abstract_inverted_index.cells	73
abstract_inverted_index.could	227
abstract_inverted_index.early	11
abstract_inverted_index.event	12
abstract_inverted_index.lumen	37
abstract_inverted_index.model	48
abstract_inverted_index.side.	108
abstract_inverted_index.sides	117
abstract_inverted_index.tight	139
abstract_inverted_index.using	86, 121, 129, 145
abstract_inverted_index.which	235
abstract_inverted_index.while	159
abstract_inverted_index.(IL)-6	178
abstract_inverted_index.CACO-2	70
abstract_inverted_index.IL-1β	186
abstract_inverted_index.apical	107, 114, 167
abstract_inverted_index.cells,	80
abstract_inverted_index.cells.	191, 213
abstract_inverted_index.driven	23
abstract_inverted_index.factor	182
abstract_inverted_index.immune	79
abstract_inverted_index.likely	22, 219
abstract_inverted_index.liquid	122
abstract_inverted_index.model.	246
abstract_inverted_index.stream	41
abstract_inverted_index.tested	98
abstract_inverted_index.tumour	180
abstract_inverted_index.zonula	207
abstract_inverted_index.(TEER).	95
abstract_inverted_index.24-hour	119
abstract_inverted_index.RESULTS	151
abstract_inverted_index.barrier	51, 60, 82, 217, 242
abstract_inverted_index.effect.	224
abstract_inverted_index.explain	237
abstract_inverted_index.gut,”	7
abstract_inverted_index.imaging	194
abstract_inverted_index.improve	200
abstract_inverted_index.induced	62, 85
abstract_inverted_index.leading	42
abstract_inverted_index.obesity	17
abstract_inverted_index.prevent	229
abstract_inverted_index.release	126, 188
abstract_inverted_index.Cytokine	125
abstract_inverted_index.However,	192, 225
abstract_inverted_index.assessed	144
abstract_inverted_index.compared	210
abstract_inverted_index.confocal	149, 193
abstract_inverted_index.detected	172
abstract_inverted_index.function	218
abstract_inverted_index.imaging.	150
abstract_inverted_index.improved	155, 216
abstract_inverted_index.junction	140
abstract_inverted_index.necrosis	181
abstract_inverted_index.presence	239
abstract_inverted_index.products	29
abstract_inverted_index.proteins	142
abstract_inverted_index.remained	164
abstract_inverted_index.residual	241
abstract_inverted_index.revealed	195
abstract_inverted_index.selected	138
abstract_inverted_index.staining	147
abstract_inverted_index.“leaky	6
abstract_inverted_index.(p=0.009)	154
abstract_inverted_index.(p=0.051)	187
abstract_inverted_index.Increased	1
abstract_inverted_index.claudin-2	205
abstract_inverted_index.decreased	176
abstract_inverted_index.diabetes.	19
abstract_inverted_index.low-grade	44
abstract_inverted_index.measuring	91
abstract_inverted_index.multiplex	131
abstract_inverted_index.occludin,	204
abstract_inverted_index.so-called	5
abstract_inverted_index.treatment	120
abstract_inverted_index.(mesoscale	134
abstract_inverted_index.CONCLUSION	214
abstract_inverted_index.Expression	136
abstract_inverted_index.OBJECTIVES	0
abstract_inverted_index.claudin-2,	234
abstract_inverted_index.co-culture	47
abstract_inverted_index.considered	9
abstract_inverted_index.determined	128
abstract_inverted_index.electrical	93
abstract_inverted_index.epithelium	77
abstract_inverted_index.intestinal	2, 36, 50, 76
abstract_inverted_index.mechanisms	58
abstract_inverted_index.properties	102
abstract_inverted_index.protection	61
abstract_inverted_index.quantified	89, 112
abstract_inverted_index.resistance	94
abstract_inverted_index.LPS-induced	212
abstract_inverted_index.METHODOLOGY	67
abstract_inverted_index.application	104
abstract_inverted_index.basolateral	116
abstract_inverted_index.co-cultured	69
abstract_inverted_index.development	15
abstract_inverted_index.discovery).	135
abstract_inverted_index.dysfunction	52, 83, 243
abstract_inverted_index.interleukin	177
abstract_inverted_index.occludens-1	208
abstract_inverted_index.characterize	56
abstract_inverted_index.localization	202, 230
abstract_inverted_index.permeability	3
abstract_inverted_index.pore-forming	233
abstract_inverted_index.representing	74
abstract_inverted_index.(p<0.0001)	184
abstract_inverted_index.Dexamethasone	96, 109, 152, 174, 215
abstract_inverted_index.concentration	110
abstract_inverted_index.dexamethasone	163, 197
abstract_inverted_index.inflammation.	45
abstract_inverted_index.respectively,	81
abstract_inverted_index.significantly	153, 175
abstract_inverted_index.spectrometry.	124
abstract_inverted_index.translocation	26
abstract_inverted_index.(p<0.0001),	179
abstract_inverted_index.basolaterally.	173
abstract_inverted_index.bacteria-derived	28
abstract_inverted_index.trans-epithelial	92
abstract_inverted_index.anti-inflammatory	65, 223
abstract_inverted_index.chemiluminescence	132
abstract_inverted_index.barrier-protective	101
abstract_inverted_index.immunocytochemical	146
abstract_inverted_index.lipopolysaccharide	32
abstract_inverted_index.chromatography/mass	123
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	6
citation_normalized_percentile.value	0.30045824
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False