Using gene complementation to identify a SulP-family bicarbonate transporter in an N2-fixing cyanobacterial endosymbiont of an open ocean diatom Article Swipe
Mercedes Nieves‐Morión
,
Rubén Romero-García
,
Sepehr Bardi
,
Luis López‐Maury
,
Martin Hagemann
,
Enrique Flores
,
Rachel A. Foster
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.01.10.573558
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.01.10.573558
Diatom-Diazotrophic Associations (DDAs) contribute significantly to new and primary production in the world’s oceans, yet the understanding of how production is sustained is poorly resolved. These symbioses involve diatoms and N 2 -fixing, heterocyst-forming cyanobacteria of the genus Richelia , both partners being photosynthetic. Richelia euintracellularis resides in the cytoplasm of Hemiaulus hauckii , whereas Richelia intracellularis is periplasmic in Rhizosolenia clevei . In the ocean, bicarbonate is taken up by phytoplankton to provide CO 2 for photosynthesis. The genomes of both Richelia endobionts (ReuHH01 and RintRC01, respectively) contain genes encoding SulP-family proteins, which are oxyanion transporters. To study the possible involvement of these transporters in bicarbonate uptake, we used complementation of a Synechocystis sp. PCC 6803 mutant with its five CO 2 uptake systems inactivated, which is unable to grow in air levels of CO 2 . Three genes from RintRC01 and one gene and a DNA fragment containing four partial gene sequences from ReuHH01 were chemically synthesized, cloned under the control of a strong gene promoter and incorporated in the chromosome of the Synechocystis mutant. One gene from RintRC01, RintRC_3892, complemented the Synechocystis mutant to grow with air levels of CO 2 or with low bicarbonate concentrations. The complemented strain showed strong sodium-dependent, low affinity bicarbonate uptake, which, together with phylogenetic analyses, identified RintRC_3892 as a BicA protein. Additionally, RintRC_3892 transcripts were consistently detected in environmental samples from three ocean basins. No evidence for a bicarbonate transporter was found, however, for ReuHH01, suggesting different strategies for inorganic carbon uptake in the periplasmic and cytoplasmic endobionts.
Related Topics
Concepts
Complementation
Biology
Periplasmic space
Cyanobacteria
Synechocystis
Mutant
Gene
Biochemistry
Pyrenoid
Bicarbonate
Gene cluster
Photosynthesis
Genetics
Chloroplast
Bacteria
Escherichia coli
Endocrinology
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2024.01.10.573558
- https://www.biorxiv.org/content/biorxiv/early/2024/01/10/2024.01.10.573558.full.pdf
- OA Status
- green
- References
- 50
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4390719503
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4390719503Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2024.01.10.573558Digital Object Identifier
- Title
-
Using gene complementation to identify a SulP-family bicarbonate transporter in an N2-fixing cyanobacterial endosymbiont of an open ocean diatomWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-01-10Full publication date if available
- Authors
-
Mercedes Nieves‐Morión, Rubén Romero-García, Sepehr Bardi, Luis López‐Maury, Martin Hagemann, Enrique Flores, Rachel A. FosterList of authors in order
- Landing page
-
https://doi.org/10.1101/2024.01.10.573558Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2024/01/10/2024.01.10.573558.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2024/01/10/2024.01.10.573558.full.pdfDirect OA link when available
- Concepts
-
Complementation, Biology, Periplasmic space, Cyanobacteria, Synechocystis, Mutant, Gene, Biochemistry, Pyrenoid, Bicarbonate, Gene cluster, Photosynthesis, Genetics, Chloroplast, Bacteria, Escherichia coli, EndocrinologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- References (count)
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50Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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