Validation of mailed <i>via</i> postal service dried blood spot cards on commercially available HIV testing systems Article Swipe
Tsunefusa Hayashida
,
Misao Takano
,
K. Tsuchiya
,
Takahiro Aoki
,
Hiroyuki Gatanaga
,
Noriyo Kaneko
,
Shinichi Oka
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.35772/ghm.2021.01105
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.35772/ghm.2021.01105
The demand for HIV testing using dried blood spots (DBS) has increased recently. However, DBS is not an approved sample for HIV testing in Japan. This study examined the validation of HIV testing with DBS, prepared at the laboratory or remotely and mailed via postal service to the laboratory. DBS were punched out from a 5.5 mm diameter circle on filter paper, then eluted with 600 μL of phosphate buffered saline overnight at 4℃, and analyzed by Lumipulse S HIVAg/Ab (LUM). The mean LUM count of DBS was 237.4-times diluted compared to titrated plasma. Repeated sample testing showed that although LUM count of DBS decreased slightly with increase in sample storage time (up to one month), it did not affect the result of HIV testing with DBS. Based on testing of 50 HIV+ confirmed cases and 50 HIV- persons, the estimated sensitivity was 98% (49/50) with a specificity of 100% when the cut-off value is 0.5. The single false negative case was a patient with undetectable viral load over the last 10 years, resulting in a decrease of antibody titer below the cut-off level. In conclusion, although DBS cannot completely replace plasma in HIV testing because the sensitivity was a little lower than that of plasma, it can be potentially useful for a screening test by self-finger-prick and postal service use. This will allow people to receive HIV testing without visiting public health centers.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.35772/ghm.2021.01105
- https://www.jstage.jst.go.jp/article/ghm/3/6/3_2021.01105/_pdf
- OA Status
- bronze
- Cited By
- 3
- References
- 13
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3213808280
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3213808280Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.35772/ghm.2021.01105Digital Object Identifier
- Title
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Validation of mailed <i>via</i> postal service dried blood spot cards on commercially available HIV testing systemsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-11-13Full publication date if available
- Authors
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Tsunefusa Hayashida, Misao Takano, K. Tsuchiya, Takahiro Aoki, Hiroyuki Gatanaga, Noriyo Kaneko, Shinichi OkaList of authors in order
- Landing page
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https://doi.org/10.35772/ghm.2021.01105Publisher landing page
- PDF URL
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https://www.jstage.jst.go.jp/article/ghm/3/6/3_2021.01105/_pdfDirect link to full text PDF
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://www.jstage.jst.go.jp/article/ghm/3/6/3_2021.01105/_pdfDirect OA link when available
- Concepts
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Medicine, Dried blood spot, Human immunodeficiency virus (HIV), Titer, Dried blood, Immunology, Antibody, Chromatography, ChemistryTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2022: 3Per-year citation counts (last 5 years)
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13Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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