VARIOUS ADHERENT-INVASIVE E. COLI (AIEC) AND NON-AIEC STRAINS ISOLATED FROM CROHN’S DISEASE PATIENTS GROW DIFFERENTLY WITH RUMINOCOCCUS GNAVUS PRE-DIGESTED MUCUS AND R. GNAVUS-RELEASED PRE-DIGESTED ILEAL AND COLONIC MUCUS MONOSACCHARIDES Article Swipe

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Saori Kashiwagi , Belgin Dogan , Sebastian Perez-Orozco , Biswa Choudhury , Simon Gray , Sebastian Winter , Kenneth W. Simpson , R. Balfour Sartor ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.1093/ibd/izae020.137

BACKGROUND Mucus-digesting Ruminococcus gnavus (R. gnavus), adherent-invasive E. coli (AIEC), and hydrogen sulfide (H2S) are enriched in active Crohn’s disease (CD) patients compared to healthy subjects. Dual colonization of R. gnavus and E. coli LF82 in ex-germ-free mice enhanced distal colitis and H2S production in cecal contents. R. gnavus pre-digestion of mucus enhanced the ileal CD isolate AIEC LF82 growth and H2S production in vitro. H2S enhanced LF82 adhesion and invasion both in vitro and in vivo along with related LF82 adhesion gene expression and Caco-2 cell expression of CEACAM6, a receptor for AIEC ligand FimH. Interactions between other CD-derived AIEC/non-AIEC and R. gnavus are unknown. HYPOTHESIS Different AIEC and non-AIEC may grow and produce H2S differently using mucus digested by R. gnavus, which releases mucus-derived monosaccharides and sulfur substrates. METHODS We studied CD-derived clinical isolates of AIEC 541-15 (phylotype A), 541-1 (B1), LF82 and NRG857c (B2) and non-AIEC T75 (A) and lab strain K12 (A). M9 minimal media was supplemented with porcine gastric mucin (PGM), ileal (MIM) and colonic (MCoM) mucus isolated from germ-free mice. We measured growth kinetics and H2S production by these AIEC and non-AIEC following culture with sterile supernatants of each mucin/mucus media precultured with or without R. gnavus, monosaccharides obtained by R. gnavus digestion of mucus, and several sulfur substrates in the gut added to M9 minimal media. RESULTS AIEC, especially LF82 (B2), grew better with ileal mucus aerobically and, to a lesser extent, anaerobically than non-AIEC strains, with even greater AIEC growth with R. gnavus pre-digested mucus (Fig.1). AIEC produced more H2S with R. gnavus-precultured mucus than with mucus alone. R. gnavus digestion of PGM released mucus monosaccharides (Fig.2A). AIEC strains grew differently with each monosaccharide constituent of R. gnavus pre-digested PGM. AIEC grew better than non-AIEC with each monosaccharide in aerobic conditions, but AIEC (B2) grew better than others in all monosaccharides in anaerobic conditions (Fig.2B). Amongst mucus-relevant sulfur substrates, L-cysteine was the only substrate to enhance E. coli H2S production by all AIEC and non-AIEC strains. All mucus-relevant sulfur substrates supported R. gnavus survival for 24 hrs, while H2S sustained R. gnavus for 48 hrs in minimum media. CONCLUSIONS AIEC grew better and produced more H2S with R. gnavus pre-digested mucus suggesting that co-existence of AIEC with R. gnavus mutually support each other and may explain why they are often observed together in active CD patients. In addition, enhanced AIEC growth with ileal vs. colonic mucus may explain why AIEC are frequently isolated from ileal CD patients. AIEC phylotype growth differences with mucus substrates may explain their persistence in the ileal mucosa. Figure 1 Figure 2

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Microbiology Ruminococcus Mucus Biology Crohn's disease Disease Medicine Feces Pathology Ecology

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Type: article
Language: en
Landing Page: https://doi.org/10.1093/ibd/izae020.137
PDF: https://academic.oup.com/ibdjournal/article-pdf/30/Supplement_1/S64/56417275/izae020.137.pdf
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DOI: https://doi.org/10.1093/ibd/izae020.137

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Title: VARIOUS ADHERENT-INVASIVE E. COLI (AIEC) AND NON-AIEC STRAINS ISOLATED FROM CROHN’S DISEASE PATIENTS GROW DIFFERENTLY WITH RUMINOCOCCUS GNAVUS PRE-DIGESTED MUCUS AND R. GNAVUS-RELEASED PRE-DIGESTED ILEAL AND COLONIC MUCUS MONOSACCHARIDES

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Publication year: 2024

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Publication date: 2024-01-25

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Authors: Saori Kashiwagi, Belgin Dogan, Sebastian Perez-Orozco, Biswa Choudhury, Simon Gray, Sebastian Winter, Kenneth W. Simpson, R. Balfour Sartor

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Landing page: https://doi.org/10.1093/ibd/izae020.137

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Concepts: Microbiology, Ruminococcus, Mucus, Biology, Crohn's disease, Disease, Medicine, Feces, Pathology, Ecology

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abstract_inverted_index.growth	60, 179, 248, 399, 418
abstract_inverted_index.lesser	238
abstract_inverted_index.ligand	95
abstract_inverted_index.media.	223, 356
abstract_inverted_index.mucus,	211
abstract_inverted_index.others	306
abstract_inverted_index.strain	154
abstract_inverted_index.sulfur	129, 214, 316, 337
abstract_inverted_index.vitro.	65
abstract_inverted_index.(AIEC),	10
abstract_inverted_index.Amongst	314
abstract_inverted_index.METHODS	131
abstract_inverted_index.NRG857c	146
abstract_inverted_index.RESULTS	224
abstract_inverted_index.aerobic	298
abstract_inverted_index.between	98
abstract_inverted_index.colitis	41
abstract_inverted_index.colonic	170, 403
abstract_inverted_index.culture	190
abstract_inverted_index.disease	20
abstract_inverted_index.enhance	324
abstract_inverted_index.explain	384, 406, 424
abstract_inverted_index.extent,	239
abstract_inverted_index.gastric	164
abstract_inverted_index.gnavus,	123, 203
abstract_inverted_index.greater	246
abstract_inverted_index.healthy	25
abstract_inverted_index.isolate	57
abstract_inverted_index.minimal	158, 222
abstract_inverted_index.minimum	355
abstract_inverted_index.mucosa.	430
abstract_inverted_index.porcine	163
abstract_inverted_index.produce	115
abstract_inverted_index.related	80
abstract_inverted_index.several	213
abstract_inverted_index.sterile	192
abstract_inverted_index.strains	277
abstract_inverted_index.studied	133
abstract_inverted_index.sulfide	13
abstract_inverted_index.support	379
abstract_inverted_index.without	201
abstract_inverted_index.(Fig.1).	254
abstract_inverted_index.Abstract	0
abstract_inverted_index.CEACAM6,	90
abstract_inverted_index.adhesion	69, 82
abstract_inverted_index.clinical	135
abstract_inverted_index.compared	23
abstract_inverted_index.digested	120
abstract_inverted_index.enhanced	39, 53, 67, 397
abstract_inverted_index.enriched	16
abstract_inverted_index.gnavus),	6
abstract_inverted_index.hydrogen	12
abstract_inverted_index.invasion	71
abstract_inverted_index.isolated	173, 411
abstract_inverted_index.isolates	136
abstract_inverted_index.kinetics	180
abstract_inverted_index.measured	178
abstract_inverted_index.mutually	378
abstract_inverted_index.non-AIEC	111, 149, 188, 242, 293, 333
abstract_inverted_index.observed	389
abstract_inverted_index.obtained	205
abstract_inverted_index.patients	22
abstract_inverted_index.produced	256, 362
abstract_inverted_index.receptor	92
abstract_inverted_index.released	272
abstract_inverted_index.releases	125
abstract_inverted_index.strains,	243
abstract_inverted_index.strains.	334
abstract_inverted_index.survival	342
abstract_inverted_index.together	390
abstract_inverted_index.unknown.	106
abstract_inverted_index.(Fig.2A).	275
abstract_inverted_index.(Fig.2B).	313
abstract_inverted_index.Crohn’s	19
abstract_inverted_index.Different	108
abstract_inverted_index.addition,	396
abstract_inverted_index.anaerobic	311
abstract_inverted_index.contents.	47
abstract_inverted_index.digestion	209, 269
abstract_inverted_index.following	189
abstract_inverted_index.germ-free	175
abstract_inverted_index.patients.	394, 415
abstract_inverted_index.phylotype	417
abstract_inverted_index.subjects.	26
abstract_inverted_index.substrate	322
abstract_inverted_index.supported	339
abstract_inverted_index.sustained	348
abstract_inverted_index.(phylotype	140
abstract_inverted_index.BACKGROUND	1
abstract_inverted_index.CD-derived	100, 134
abstract_inverted_index.HYPOTHESIS	107
abstract_inverted_index.L-cysteine	318
abstract_inverted_index.conditions	312
abstract_inverted_index.especially	226
abstract_inverted_index.expression	84, 88
abstract_inverted_index.frequently	410
abstract_inverted_index.production	44, 63, 183, 328
abstract_inverted_index.substrates	215, 338, 422
abstract_inverted_index.suggesting	370
abstract_inverted_index.CONCLUSIONS	357
abstract_inverted_index.aerobically	234
abstract_inverted_index.conditions,	299
abstract_inverted_index.constituent	283
abstract_inverted_index.differences	419
abstract_inverted_index.differently	117, 279
abstract_inverted_index.mucin/mucus	196
abstract_inverted_index.persistence	426
abstract_inverted_index.precultured	198
abstract_inverted_index.substrates,	317
abstract_inverted_index.substrates.	130
abstract_inverted_index.Interactions	97
abstract_inverted_index.Ruminococcus	3
abstract_inverted_index.co-existence	372
abstract_inverted_index.colonization	28
abstract_inverted_index.ex-germ-free	37
abstract_inverted_index.pre-digested	252, 287, 368
abstract_inverted_index.supernatants	193
abstract_inverted_index.supplemented	161
abstract_inverted_index.AIEC/non-AIEC	101
abstract_inverted_index.anaerobically	240
abstract_inverted_index.mucus-derived	126
abstract_inverted_index.pre-digestion	50
abstract_inverted_index.monosaccharide	282, 296
abstract_inverted_index.mucus-relevant	315, 336
abstract_inverted_index.Mucus-digesting	2
abstract_inverted_index.monosaccharides	127, 204, 274, 309
abstract_inverted_index.adherent-invasive	7
abstract_inverted_index.gnavus-precultured	261
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	8
citation_normalized_percentile.value	0.01899365
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False