Gonçalo Amarante Guimarães Pereira
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View article: Impact of CRISPRi-Mediated Titration of <i>GPD</i> Genes on the Fermentative Performance of <i>S. cerevisiae</i>
Impact of CRISPRi-Mediated Titration of <i>GPD</i> Genes on the Fermentative Performance of <i>S. cerevisiae</i> Open
Glycerol is one of the main byproducts in ethanol fermentation due to its importance in redox balance and response to osmotic stress in Saccharomyces cerevisiae. Since its production diverts carbon from alcohol production, traditional gene…
View article: A rational approach for multicopy delta integration in <i>Saccharomyces cerevisiae</i> targeting a conserved Ty1 LTR sequence
A rational approach for multicopy delta integration in <i>Saccharomyces cerevisiae</i> targeting a conserved Ty1 LTR sequence Open
Genomic integration is a foundation in yeast-based cell factories, ensuring phenotypic stability and adequate gene dosage to sustain desired metabolic fluxes. Retrotransposon-associated sequences are attractive genomic integration targets …
View article: An integrated microbiological analysis of the soil and rhizosphere of Agave spp. under minimum technological input farming systems
An integrated microbiological analysis of the soil and rhizosphere of Agave spp. under minimum technological input farming systems Open
The expansion of arid and semi-arid regions, consequent to the intensification of desertification processes attributable to global warming, exerts a deleterious effect on agricultural production of energy crops, with current estimates indi…
View article: Epistatic mutations in ISC metabolism synergize with cell cycle regulation and the PPP to enhance xylose fermentation and acetic acid tolerance in industrial yeast
Epistatic mutations in ISC metabolism synergize with cell cycle regulation and the PPP to enhance xylose fermentation and acetic acid tolerance in industrial yeast Open
Efficient utilization of complex biomass-derived sugars and tolerance to inhibitors are key requirements for the viability of lignocellulosic-based biorefineries. In this study, a two-stage evolution of an industrial yeast strain engineere…
View article: Epistatic mutations in ISC metabolism synergize with cell cycle regulation and the PPP to enhance xylose fermentation and acetic acid tolerance in industrial yeast
Epistatic mutations in ISC metabolism synergize with cell cycle regulation and the PPP to enhance xylose fermentation and acetic acid tolerance in industrial yeast Open
Efficient utilization of complex biomass-derived sugars and tolerance to inhibitors are key requirements for the viability of lignocellulosic-based biorefineries. In this study, a two-stage evolution of an industrial yeast strain engineere…
View article: From Semi-Arid Biomass to Carbon Sequestration: Role of Pyrolysis Atmosphere and Temperature on Agave-Derived Biochar Properties
From Semi-Arid Biomass to Carbon Sequestration: Role of Pyrolysis Atmosphere and Temperature on Agave-Derived Biochar Properties Open
Biochar has emerged as a scalable negative emission technology with promising agronomic and environmental benefits. In this context, arid and semi-arid regions represent promising zones for carbon removal through soil biochar application, …
View article: Blockchain and Accounting: Contemporary Benefits and Challenges
Blockchain and Accounting: Contemporary Benefits and Challenges Open
The rapid digital transformation has led to substantial changes in the way accounting information is generated, validated, and disclosed, creating the need for new alignments between accounting practices and emerging technologies. This stu…
View article: Unveiling molecular mechanisms of strobilurin resistance in the cacao pathogen Moniliophthora perniciosa
Unveiling molecular mechanisms of strobilurin resistance in the cacao pathogen Moniliophthora perniciosa Open
Strobilurin fungicides inhibit mitochondrial respiration, leading to ATP depletion and oxidative stress. Although widely used in agriculture, these chemicals are ineffective against the cacao pathogen Moniliophthora perniciosa. Here, we sh…
View article: An integrated microbiological analysis of the soil and rhizosphere of Agave spp. under minimum technological input farming systems
An integrated microbiological analysis of the soil and rhizosphere of Agave spp. under minimum technological input farming systems Open
The expansion of arid and semi-arid regions, consequent to the intensification of desertification processes attributable to global warming, exerts a deleterious effect on agricultural production of energy crops, thereby precipitating crise…
View article: Engineering <i>Saccharomyces Cerevisiae</i> With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production
Engineering <i>Saccharomyces Cerevisiae</i> With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production Open
Xylose metabolism in Saccharomyces cerevisiae remains a significant bottleneck due to the difficulty in identifying functional and efficient xylose isomerases (XI). In the present study, publicly available metagenomic and metatranscriptomi…
View article: BEYOND CONTENT: THE TEACHER'S ROLE IN FACILITATING LEARNING
BEYOND CONTENT: THE TEACHER'S ROLE IN FACILITATING LEARNING Open
In order for meaningful learning to occur, the traditional conception of teaching must be transformed, shifting the focus from the teacher as a mere transmitter of knowledge to a role as a facilitator of learning and placing the student, t…
View article: Showdown in the OR: Minimally Invasive vs. Conventional Surgery for Type III Esophageal Atresia - A Systematic Review
Showdown in the OR: Minimally Invasive vs. Conventional Surgery for Type III Esophageal Atresia - A Systematic Review Open
Esophageal atresia (EA) type III, characterized by a distal tracheoesophageal fistula, requires early surgical intervention to establish esophageal continuity and prevent severe respiratory complications. Although open surgical repair via …
View article: Unveiling novel mechanisms of strobilurin resistance in the cacao pathogen<i>Moniliophthora perniciosa</i>
Unveiling novel mechanisms of strobilurin resistance in the cacao pathogen<i>Moniliophthora perniciosa</i> Open
Witches’ broom disease (WBD) is a major constraint for cacao production in the Americas. The severe socioeconomic impact of WBD encouraged the evaluation of different control strategies, including the use of strobilurin fungicides. These m…
View article: From Rags to Riches: the Fermentation Potential of Agave Leaf Residues in the Brazilian Semi-arid
From Rags to Riches: the Fermentation Potential of Agave Leaf Residues in the Brazilian Semi-arid Open
CAM plants are promising biomasses to assure energy security and biofuel supply in the current changing climate scenario. Their high sugar content and strengthened tolerance to high temperatures and droughts makes them attractive alternati…
View article: Integrating Electro-fermentation and Other Strategies to Enhance Xylitol Production From Sugarcane Hydrolysate by Saccharomyces Cerevisiae
Integrating Electro-fermentation and Other Strategies to Enhance Xylitol Production From Sugarcane Hydrolysate by Saccharomyces Cerevisiae Open
Xylitol, a naturally occurring biomolecule, is utilized in various commercial applications, with its market expected to reach USD 1.7 billion by 2028. Large-scale commercial production typically involves rigorous chemical processes, making…
View article: Unveiling genetic anchors in Saccharomyces cerevisiae: QTL mapping identifies IRA2 as a key player in ethanol tolerance and beyond
Unveiling genetic anchors in Saccharomyces cerevisiae: QTL mapping identifies IRA2 as a key player in ethanol tolerance and beyond Open
Ethanol stress in Saccharomyces cerevisiae is a well-studied phenomenon, but pinpointing specific genes or polymorphisms governing ethanol tolerance remains a subject of ongoing debate. Naturally found in sugar-rich environments, this yeas…
View article: Advancing the agave‐soil nexus approach: A systematic review
Advancing the agave‐soil nexus approach: A systematic review Open
Agave plants, found primarily in arid and semi‐arid regions, have been utilized by indigenous communities for various purposes for thousands of years. They currently serve as crops to produce alcoholic beverages and fibers. Despite their i…
View article: Exploring the pyrolysis of agave species as a novel bioenergy source: thermo-kinetics, modeling, and product composition insights
Exploring the pyrolysis of agave species as a novel bioenergy source: thermo-kinetics, modeling, and product composition insights Open
The agave plant’s economic chain generates a significant waste, which has potential for sustainable bioenergy through pyrolysis. However, the diversity and heterogeneity of chemical composition of agave species may pose challenges. This st…
View article: An atlas of rational genetic engineering strategies for improved xylose metabolism in <i>Saccharomyces cerevisiae</i>
An atlas of rational genetic engineering strategies for improved xylose metabolism in <i>Saccharomyces cerevisiae</i> Open
Xylose is the second most abundant carbohydrate in nature, mostly present in lignocellulosic material, and representing an appealing feedstock for molecule manufacturing through biotechnological routes. However, Saccharomyces cerevisiae —a…
View article: Molecular epidemiology of sisal bole rot disease suggests a potential phytosanitary crisis in Brazilian production areas
Molecular epidemiology of sisal bole rot disease suggests a potential phytosanitary crisis in Brazilian production areas Open
Sisal bole rot disease is the major phytosanitary problem of Agave plantations in Brazil. The disease is caused by a cryptic species of Aspergillus : A. welwitschiae . To date, the only way to diagnose the disease was to observe external s…
View article: Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm
Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm Open
Agaves have been a valuable resource in dryland areas for centuries, providing fibers (sisal), food, and beverages. However, the advent of synthetic fibers has led to a decrease in research on Agave , resulting in the cessation of breeding…