Berin A. Boughton
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View article: Remodelled ribosomal populations synthesize a specific proteome in proliferating plant tissue during cold
Remodelled ribosomal populations synthesize a specific proteome in proliferating plant tissue during cold Open
Plant acclimation occurs through system-wide mechanisms that include proteome shifts, some of which occur at the level of protein synthesis. All proteins are synthesized by ribosomes. Rather than being monolithic, transcript-to-protein tra…
View article: Polyamines and flg22 reshape the ribosomal protein composition of actively translating ribosomes in plants
Polyamines and flg22 reshape the ribosomal protein composition of actively translating ribosomes in plants Open
Polyamines are small, polycationic molecules with amino groups that are present in most living organisms. Studies indicate that polyamines increase general protein synthesis and are essential for efficient translation. While progress has b…
View article: Identification of plasma protein biomarkers for endometriosis and the development of statistical models for disease diagnosis
Identification of plasma protein biomarkers for endometriosis and the development of statistical models for disease diagnosis Open
STUDY QUESTION Can a panel of plasma protein biomarkers be identified to accurately and specifically diagnose endometriosis? SUMMARY ANSWER A novel panel of 10 plasma protein biomarkers was identified and validated, demonstrating strong pr…
View article: 1H NMR spectroscopic characterisation of HepG2 cells as a model metabolic system for toxicology studies
1H NMR spectroscopic characterisation of HepG2 cells as a model metabolic system for toxicology studies Open
The immortalised human hepatocellular HepG2 cell line is commonly used for toxicology studies as an alternative to animal testing due to its characteristic liver-distinctive functions. However, little is known about the baseline metabolic …
View article: The Impacts of Chitosan on Plant Root Systems and Its Potential to be Used for Controlling Fungal Diseases in Agriculture
The Impacts of Chitosan on Plant Root Systems and Its Potential to be Used for Controlling Fungal Diseases in Agriculture Open
Chitosan is a natural elicitor, used for stimulating plant growth and inducing plant defense. However, due to difficulty in monitoring root growth and activity, the effects of chitosan treatment on plant root systems have been less studied…
View article: Unravelling inulin molecules in food sources using a matrix-assisted laser desorption/ionization magnetic resonance mass spectrometry (MALDI-MRMS) pipeline
Unravelling inulin molecules in food sources using a matrix-assisted laser desorption/ionization magnetic resonance mass spectrometry (MALDI-MRMS) pipeline Open
Inulin, a polysaccharide characterized by a β-2,1 fructosyl-fructose structure terminating in a glucosyl moiety, is naturally present in plant roots and tubers. Current methods provide average degrees of polymerization (DP) but lack inform…
View article: Integrative Molecular Structure Elucidation and Construction of an Extended Metabolic Pathway Associated with an Ancient Innate Immune Response in COVID-19 Patients
Integrative Molecular Structure Elucidation and Construction of an Extended Metabolic Pathway Associated with an Ancient Innate Immune Response in COVID-19 Patients Open
We present compelling evidence for the existence of an extended innate viperin-dependent pathway, which provides crucial evidence for an adaptive response to viral agents, such as SARS-CoV-2. We show the in vivo biosynthesis of a family of…
View article: Investigation of biochemical changes in barley inoculated with Trichoderma harzianum T-22 under salt stress
Investigation of biochemical changes in barley inoculated with Trichoderma harzianum T-22 under salt stress Open
Increases in soil salinity impact growth and agricultural yield by inhibiting plant functions. Interactions of fungal endophytes with crop plants can improve tolerance and is a less expensive approach. Here, the role of Trichoderma harzian…
View article: Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant Ribosome
Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant Ribosome Open
Ribosomes are an archetypal ribonucleoprotein assembly. Due to ribosomal evolution and function, r-proteins share specific physicochemical similarities, making the riboproteome particularly suited for tailored proteome profiling methods. M…
View article: Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC–ESI–QqTOF with parallel reaction monitoring
Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC–ESI–QqTOF with parallel reaction monitoring Open
This study explores the sphingolipid class of oligohexosylceramides (OHCs), a rarely studied group, in barley ( Hordeum vulgare L.) through a new lipidomics approach. Profiling identified 45 OHCs in barley ( Hordeum vulgare L.), elucidatin…
View article: Hormonal and proteomic analyses of southern blight disease caused by <scp><i>Athelia rolfsii</i></scp> and root chitosan priming on <scp><i>Cannabis sativa</i></scp> in an in vitro hydroponic system
Hormonal and proteomic analyses of southern blight disease caused by <span><i>Athelia rolfsii</i></span> and root chitosan priming on <span><i>Cannabis sativa</i></span> in an in vitro hydroponic system Open
Southern blight disease, caused by the fungal pathogen Athelia rolfsii , suppresses plant growth and reduces product yield in Cannabis sativa agriculture. Mechanisms of pathology of this soil‐borne disease remain poorly understood, with di…
View article: Metabolism regulates muscle stem cell self-renewal by connecting the microenvironment and histone acetylation
Metabolism regulates muscle stem cell self-renewal by connecting the microenvironment and histone acetylation Open
SUMMARY Skeletal muscle contains a resident population of somatic stem cells capable of both self-renewal and differentiation. The signals that regulate this important decision have yet to be fully elucidated. Here we use metabolomics and …
View article: SARS-CoV-2 Infection Biomarkers Reveal an Extended RSAD2 Dependant Metabolic Pathway
SARS-CoV-2 Infection Biomarkers Reveal an Extended RSAD2 Dependant Metabolic Pathway Open
We present compelling evidence for the existence of an extended innate viperin dependent pathway which provides crucial evidence for an adaptive response to viral agents like SARS-CoV-2. We show the in vivo biosynthesis of a family of endo…
View article: Diverse organ-specific localisation of a chemical defence, cyanogenic glycosides, in flowers of eleven species of Proteaceae
Diverse organ-specific localisation of a chemical defence, cyanogenic glycosides, in flowers of eleven species of Proteaceae Open
Floral chemical defence strategies remain under-investigated, despite the significance of flowers to plant fitness. We used cyanogenic glycosides (CNglycs)—constitutive secondary metabolites that deter herbivores by releasing hydrogen cyan…
View article: Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of <i>Cannabis sativa</i>
Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of <i>Cannabis sativa</i> Open
Fungal pathogens pose a major threat to Cannabis sativa production, requiring safe and effective management procedures to control disease. Chitin and chitosan are natural molecules that elicit plant defense responses. Investigation of thei…
View article: Remodelled Ribosomes Synthesise a Specific Proteome in Proliferating Plant Tissue during Cold
Remodelled Ribosomes Synthesise a Specific Proteome in Proliferating Plant Tissue during Cold Open
Plant acclimation to low temperatures occurs through system-wide mechanisms that include proteome shifts, some of which occur at the level of protein synthesis. All proteins are synthesised by ribosomes. Rather than being monolithic, trans…
View article: Longitudinal spatial mapping of lipid metabolites reveals pre-symptomatic changes in the hippocampi of Huntington's disease transgenic mice
Longitudinal spatial mapping of lipid metabolites reveals pre-symptomatic changes in the hippocampi of Huntington's disease transgenic mice Open
In Huntington's disease (HD), a key pathological feature includes the development of inclusion-bodies of fragments of the mutant huntingtin protein in the neurons of the striatum and hippocampus. To examine the molecular changes associated…
View article: Large-Scale Interlaboratory DI-FT-ICR MS Comparability Study Employing Various Systems
Large-Scale Interlaboratory DI-FT-ICR MS Comparability Study Employing Various Systems Open
Ultrahigh resolution mass spectrometry (UHR-MS) coupled with direct infusion (DI) electrospray ionization offers a fast solution for accurate untargeted profiling. Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers have …
View article: Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of<i>Cannabis sativa</i>
Effects of chitin and chitosan on root growth, biochemical defense response and exudate proteome of<i>Cannabis sativa</i> Open
Fungal pathogens pose a major threat to Cannabis sativa production, requiring safe and effective management procedures to control disease. Chitin and chitosan are natural molecules that elicit plant defense responses. Investigation of thei…
View article: KineticMSI, an R-based framework for relative quantification of spatial isotopic incorporation in mass spectrometry imaging experiments
KineticMSI, an R-based framework for relative quantification of spatial isotopic incorporation in mass spectrometry imaging experiments Open
Kinetic mass spectrometry imaging (kMSI) integrates imaging-MS with stable isotope labelling to elucidate metabolic fluxes in a spatiotemporal manner. kMSI studies are hampered by high volumes of complex data and a lack of computational wo…
View article: Measures of insulin sensitivity, leptin, and adiponectin concentrations in cats in diabetic remission compared to healthy control cats
Measures of insulin sensitivity, leptin, and adiponectin concentrations in cats in diabetic remission compared to healthy control cats Open
Objectives Firstly, to compare differences in insulin, adiponectin, leptin, and measures of insulin sensitivity between diabetic cats in remission and healthy control cats, and determine whether these are predictors of diabetic relapse. Se…
View article: Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa
Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa Open
Background Plant growth devices, for example, rhizoponics, rhizoboxes, and ecosystem fabrication (EcoFAB), have been developed to facilitate studies of plant root morphology and plant-microbe interactions in controlled laboratory settings.…
View article: Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in <i>n</i>-Dimensions (SHY-<i>n</i>): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection
Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in <i>n</i>-Dimensions (SHY-<i>n</i>): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection Open
SARS-CoV-2 infection causes a significant reduction in lipoprotein-bound serum phospholipids give rise to supramolecular phospholipid composite (SPC) signals observed in diffusion and relaxation edited 1H NMR spectra. To characterize the c…
View article: Additional file 7 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa
Additional file 7 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa Open
Additional file 7: Gene and primer details of six C. sativa genes detected in this study.
View article: Additional file 10 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa
Additional file 10 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa Open
Additional file 10: 3D design file of the top external structural frame.
View article: Additional file 11 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa
Additional file 11 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa Open
Additional file 11: 3D design file of the stand.
View article: Additional file 13 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa
Additional file 13 of Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa Open
Additional file 13: 3D design file of right window shutter.