Alwyn Dady
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Engineering fluorescent reporters in human pluripotent stem cells and strategies for live imaging human neurogenesis Open
Investigation of cell behaviour and cell biological processes underlying human development is facilitated by the creation of fluorescent reporters in human pluripotent stem cells, which can be differentiated into cell types of choice. Here…
Genetic variability in proteoglycan biosynthetic genes reveals new facets of heparan sulfate diversity. Open
Heparan sulfate (HS) and chondroitin sulfate (CS) proteoglycans (PG) consist of a core protein to which the glycosaminoglycan (GAG) chains, HS or CS, are attached through a common linker tetrasaccharide. In the extracellular space, they ar…
Engineering fluorescent reporters in human pluripotent cells and strategies for live imaging human neurogenesis Open
Investigation of cell behaviour and cell biological processes underlying human development is facilitated by creation of fluorescent reporters in human pluripotent stem cells, which can be differentiated into cell types of choice. Here we …
Non-canonical nuclear function of glutaminase cooperates with Wnt signaling to drive EMT during neural crest development Open
Metabolic reprograming has been linked to epithelial-to-mesenchymal transition (EMT) in cancer cells, but how it influences EMT in normal cells remains largely unknown. Here we explored how metabolism impacts delamination and migration of …
Glucose oxidation drives trunk neural crest cell development and fate Open
Bioenergetic metabolism is a key regulator of cellular function and signaling, but how it can instruct the behavior of cells and their fate during embryonic development remains largely unknown. Here, we investigated the role of glucose met…
A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis Open
Dynamic contacts between cells within the developing neuroepithelium are poorly understood but play important roles in cell and tissue morphology and cell signalling. Here, using live-cell imaging and electron-microscopy we reveal multiple…
Author response: Human spinal cord in vitro differentiation pace is initially maintained in heterologous embryonic environments Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Species-specific diffe…
A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis Open
Dynamic contacts between cells within the developing neuroepithelium are poorly understood but play important roles in cell and tissue morphology and cell signalling. Here, using live-cell imaging and electron microscopy we reveal multiple…
Human spinal cord differentiation proceeds rapidly in vitro and only initially maintains differentiation pace in a heterologous environment Open
Species-specific differentiation pace in in vitro assays indicates that some aspects of neural differentiation are driven by cell-autonomous processes. Here we describe a novel in vitro human neural rosette assay that recapitulates the tem…
Wnt regulates amino acid transporter <i>Slc7a5</i> and so constrains the integrated stress response in mouse embryos Open
Amino acids are essential for cellular metabolism, and it is important to understand how nutrient supply is coordinated with changing energy requirements during embryogenesis. Here, we show that the amino acid transporter Slc7a5 / Lat1 is …
Neural differentiation, selection and transcriptomic profiling of human neuromesodermal progenitors-like cells in vitro Open
Robust protocols for directed differentiation of human pluripotent cells are required to determine whether mechanisms operating in model organisms are relevant to our own development. Recent work in vertebrate embryos has identified neurom…
Cadherin interplay during neural crest segregation from the non‐neural ectoderm and neural tube in the early chick embryo Open
Background : In the avian embryo, neural crest (NC) progenitors arise in the neuroectoderm during gastrulation, long before their dissemination. Although the gene regulatory network involved in NC specification has been deciphered, the mec…